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Total Antioxidant Status (TAS) Colorimetric Assay Kit

Citations(7)

Cat.No.:E-BC-K801-M
Detection instrument: Microplate reader(650 nm-670 nm,optimum wavelength: 660 nm)

Detection method: Colorimetric method

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Protocols for metabolism assays

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Detection principle

ABTS is oxidized to green ABTS•+ by appropriate oxidant, which can be reduced to colorless ABTS in the presence of antioxidants. The TAS of the sample can be determined and calculated by measuring the absorbance of ABTS•+ at 660 nm. Trolox is an analog of VE and has a similar antioxidant state to that of VE. Trolox is used as a reference substance for total antioxidant status.

Performance characteristics

Synonyms	TAS
Sample type	serum, plasma, urine, cellular supernatant, animal, plant tissue
Sensitivity	0.23 mmol Trolox Equiv./L
Detection range	0.23-2 mmol Trolox Equiv./L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	30 min
Precision	Average inter-assay CV: 7%Average intra-assay CV: 4.600%
Other instruments required	Micropipettor, 37℃ incubator.
Other reagents required	60% Ethanol
Storage	-20℃
Valid period	12 months

Images

Different subscript letters indicate significant differences among the groups. (P<0.05)

X Zhao et al investigate the function of quercetin for reducing ethanol-induced hepatocyte pyroptosis. Total antioxidants status (TAS) of human L02 cell was determined using TAS colorimetric assay kit (E-BC-K801-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.23-2 mmol Trolox Equiv. /L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
10% Mouse liver tissue homogenate	1
10% Rat liver tissue homogenate	1
10% Rat lung tissue homogenate	1
Molt4 cellar supernatant	1
Human urine	8-10
Mouse serum	1
Human serum	1
Human saliva	1

Note: The diluent is 60% Ethanol.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

ORAL DISEASES (2022) IF: 3.511

Resveratrol protects renal damages induced by periodontitis via preventing mitochondrial dysfunction in rats

DOI: 10.1111/odi.14148
PMID: 35146845	Sample: Kidney

JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2022) IF: 3.652

Zoledronic acid-induced oxidative damage and endoplasmic reticulum stress-mediated apoptosis in human embryonic kidney (HEK-293) cells

DOI: 10.1002/jbt.23083
PMID: 35587103	Sample: Hek-293 Cell

BIOLOGICAL TRACE ELEMENT RESEARCH (2022) IF: 3.738

Selenoprotein Gene mRNA Expression Evaluation During Renal Ischemia–Reperfusion Injury in Rats and Ebselen Intervention Effects

DOI: 10.1007/s12011-022-03275-7
	Sample: Kidney

International Journal of Environmental Research and Public Health (2022) IF: 4.614

6-Week Supplementation with Tribulus terrestris L. to Trained Male CrossFit® Athletes on Muscle, Inflammation, and Antioxidant Biomarkers: A Randomized, Single-Blind, Placebo-Controlled Trial

DOI: 10.3390/ijerph192316158
	Sample: Blood

Biology-Basel (2021) IF: 5.079

Cellular Stress Pathways Are Linked to Acetamiprid-Induced Apoptosis in SH-SY5Y Neural Cells

DOI: 10.3390/biology10090820
	Sample: Sh-Sy5Y Cell

Metabolites (2022) IF: 5.581

The Protective and Therapeutic Anti-Alzheimer Potential of Olea europaea L. cv. Picual: An In Silico and In Vivo Study

DOI: 10.3390/metabo12121178
	Sample: Serum

Oxidative Medicine and Cellular Longevity (2022) IF: 7.31

Quercetin Protects Ethanol-Induced Hepatocyte Pyroptosis via Scavenging Mitochondrial ROS and Promoting PGC-1α-Regulated Mitochondrial Homeostasis in L02 Cells

DOI: 10.1155/2022/4591134
	Sample: L02 Cell

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Reviews/Q&A

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Reviews
Q&A

B***********zSubmitted [ Sep 12 2024 ]

Asked: Hi, I am having difficulty calculating the kit protocol. Can you help me with this?

adminSubmitted [ Sep 12 2024 ]

Answered:

İ**mSubmitted [ Aug 19 2024 ]

Asked: Can we use it on rats for serum and plasma

adminSubmitted [ Aug 19 2024 ]

Answered: Hi, Thanks for your checking. We confirm the TAS kit could be applied on rats for serum and plasma. For more question, please feel free to contact us. Regards,

I*aSubmitted [ Jun 25 2024 ]

Asked: How stable are the reagents after opening the kit? How many times can the reagents be used (freeze-thaw)? Can I re-use the microplate if few wells are going to be used at a time? Thank you!

adminSubmitted [ Jun 25 2024 ]

Answered: 1. After opening the kit, ensure that the reagent is sealed well and can be stored at -20℃ for 12 months. 2. Repeated freezing and thawing may have a certain impact on the properties of the reagent. If multiple tests are required, it is recommended to aliquoted storage at -20° C. 3. The microplate can not be reused. If the microplate is not enough, the microplate of this kit can be replaced by ordinary 96-well transparent plate.

U********NSubmitted [ Apr 16 2024 ]

Asked: Greetings, Are TAS and TOS kits species specific? Can fish serum and muscle tissue be used?

adminSubmitted [ Apr 16 2024 ]

Answered: E-BC-K801-M E-BC-K802-M can be used to detect serum and tissue samples, but we have only verified some routine serum and tissue samples, such as human serum, rat serum, bovine serum, and rat liver and brain tissue. In theory, TAS and TOS in fish serum tissue and muscle tissue are higher than the sensitivity of the kit can be detected.

U********NSubmitted [ Apr 16 2024 ]

Asked: Greetings, Are TAS and TOS kits species specific? Can fish serum and muscle tissue be used?

adminSubmitted [ Apr 16 2024 ]

U********NSubmitted [ Apr 16 2024 ]

Asked: Greetings, Are TAS and TOS kits species specific? Can fish serum and muscle tissue be used?

adminSubmitted [ Apr 16 2024 ]

D****oSubmitted [ Feb 16 2024 ]

Asked: I\'m having so much difficulty calculating TAS values (and also TOS, I bought the two kits). I\'ve already tried everything, and the values result in something very out of my understanding capacity. I would like to know if I must consider the sample\'s OD values or the mmol/L concentration. For example, when my standard curve is on 0 concentration I have a 0,049 OD concentration. Since this value is too when compared to the sample\'s OD, my results always give negative. How to proceed? Could you please assist in this or have any tutorial video that could explain more easily how to obtain these values? Thank you in advance.

adminSubmitted [ Feb 16 2024 ]

Answered: I have sent the email to you. Please check about it.

SGSubmitted [ Oct 17 2023 ]

Asked: Can you please help out in calculation part?

adminSubmitted [ Oct 17 2023 ]

Answered: Hello sir,thanks for your support.We will reply you by E-mail.

P**********uSubmitted [ Sep 26 2023 ]

Asked: Am doing a research ... determination of total Antioxidant status of sickle cell anaemia children. Am a post graduate medical doctor. Can you tell me about this kit. Can it be use to evaluate the status of the patients

adminSubmitted [ Sep 26 2023 ]

Answered: Hi Prince, E-BC-K801-M can be used to detect total antioxidant status in serum (plasma), urine, animal and plant tissues, and cell supernatant samples. If your sample type is one of these, then the kit is available!

R*****nSubmitted [ Mar 27 2023 ]

Asked: Hello, I have one doubt after taking the second reading how should we need to calculate the mean value of TOS & TAOS, Can you please share some videos or handouts to calculate the results of TOS & TAOS.

adminSubmitted [ Mar 27 2023 ]

Answered: We will reply you by E-mail.

E*oSubmitted [ Nov 24 2022 ]

Asked: Hello, what is the normal range of this kit?

M*****aSubmitted [ Aug 25 2022 ]

Asked: Does the tissue sample need to measure protein concentrations?

adminSubmitted [ Aug 25 2022 ]

Answered: No, there is no need to detect the protein concentration. You can calculate through wet weight directly.

M*****aSubmitted [ Aug 17 2022 ]

Asked: Can the kits be used for cell sample? If yes, How many cells do we need?

adminSubmitted [ Aug 17 2022 ]

Answered: It is suitable for cell sample and the amout of the cell is at least 2*10^6.

M*****aSubmitted [ Aug 15 2022 ]

Asked: Can the kits be used for cell sample? If yes, How many cells do we need?

adminSubmitted [ Aug 15 2022 ]

Answered: It is suitable for cell sample and the amout of the cell is at least 2*10^6.

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