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Cat.No.:E-BC-K801-M
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Synonyms | TAS |
Sample type | serum, plasma, urine, cellular supernatant, animal, plant tissue |
Sensitivity | 0.23 mmol Trolox Equiv./L |
Detection range | 0.23-2 mmol Trolox Equiv./L |
Detection method | Colorimetric method |
Assay type | Quantitative |
Assay time | 30 min |
Precision | Average inter-assay CV: 7%Average intra-assay CV: 4.600% |
Other instruments required | Micropipettor, 37℃ incubator. |
Other reagents required | 60% Ethanol |
Storage | -20℃ |
Valid period | 12 months |
Different subscript letters indicate significant differences among the groups. (P<0.05)
X Zhao et al investigate the function of quercetin for reducing ethanol-induced hepatocyte pyroptosis. Total antioxidants status (TAS) of human L02 cell was determined using TAS colorimetric assay kit (E-BC-K801-M).
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.23-2 mmol Trolox Equiv. /L).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type |
Dilution factor |
10% Mouse liver tissue homogenate |
1 |
10% Rat liver tissue homogenate |
1 |
10% Rat lung tissue homogenate |
1 |
Molt4 cellar supernatant |
1 |
Human urine |
8-10 |
Mouse serum |
1 |
Human serum |
1 |
Human saliva |
1 |
Note: The diluent is 60% Ethanol.
DOI: 10.1155/2022/4591134 |
|
Sample: L02 cell |
Sample: SH-SY5Y cell |
Sample: serum |
Sample: blood |
DOI: 10.1111/odi.14148 |
|
PMID: 35146845 |
Sample: kidney |
DOI: 10.1002/jbt.23083 |
|
PMID: 35587103 |
Sample: HEK-293 cell |
Sample: kidney |
U********NSubmitted [ Apr 16 2024 ]
Asked: Greetings, Are TAS and TOS kits species specific? Can fish serum and muscle tissue be used?
Reply
adminSubmitted [ Apr 16 2024 ]
Answered: E-BC-K801-M E-BC-K802-M can be used to detect serum and tissue samples, but we have only verified some routine serum and tissue samples, such as human serum, rat serum, bovine serum, and rat liver and brain tissue. In theory, TAS and TOS in fish serum tissue and muscle tissue are higher than the sensitivity of the kit can be detected.
U********NSubmitted [ Apr 16 2024 ]
Asked: Greetings, Are TAS and TOS kits species specific? Can fish serum and muscle tissue be used?
Reply
adminSubmitted [ Apr 16 2024 ]
Answered: E-BC-K801-M E-BC-K802-M can be used to detect serum and tissue samples, but we have only verified some routine serum and tissue samples, such as human serum, rat serum, bovine serum, and rat liver and brain tissue. In theory, TAS and TOS in fish serum tissue and muscle tissue are higher than the sensitivity of the kit can be detected.
U********NSubmitted [ Apr 16 2024 ]
Asked: Greetings, Are TAS and TOS kits species specific? Can fish serum and muscle tissue be used?
Reply
adminSubmitted [ Apr 16 2024 ]
Answered: E-BC-K801-M E-BC-K802-M can be used to detect serum and tissue samples, but we have only verified some routine serum and tissue samples, such as human serum, rat serum, bovine serum, and rat liver and brain tissue. In theory, TAS and TOS in fish serum tissue and muscle tissue are higher than the sensitivity of the kit can be detected.
D****oSubmitted [ Feb 16 2024 ]
Asked: I\'m having so much difficulty calculating TAS values (and also TOS, I bought the two kits). I\'ve already tried everything, and the values result in something very out of my understanding capacity. I would like to know if I must consider the sample\'s OD values or the mmol/L concentration. For example, when my standard curve is on 0 concentration I have a 0,049 OD concentration. Since this value is too when compared to the sample\'s OD, my results always give negative. How to proceed? Could you please assist in this or have any tutorial video that could explain more easily how to obtain these values? Thank you in advance.
Reply
adminSubmitted [ Feb 16 2024 ]
Answered: I have sent the email to you. Please check about it.
SGSubmitted [ Oct 17 2023 ]
Asked: Can you please help out in calculation part?
Reply
adminSubmitted [ Oct 17 2023 ]
Answered: Hello sir,thanks for your support.We will reply you by E-mail.
P**********uSubmitted [ Sep 26 2023 ]
Asked: Am doing a research ... determination of total Antioxidant status of sickle cell anaemia children. Am a post graduate medical doctor. Can you tell me about this kit. Can it be use to evaluate the status of the patients
Reply
adminSubmitted [ Sep 26 2023 ]
Answered: Hi Prince, E-BC-K801-M can be used to detect total antioxidant status in serum (plasma), urine, animal and plant tissues, and cell supernatant samples. If your sample type is one of these, then the kit is available!
R*****nSubmitted [ Mar 27 2023 ]
Asked: Hello, I have one doubt after taking the second reading how should we need to calculate the mean value of TOS & TAOS, Can you please share some videos or handouts to calculate the results of TOS & TAOS.
Reply
adminSubmitted [ Mar 27 2023 ]
Answered: We will reply you by E-mail.
E*oSubmitted [ Nov 24 2022 ]
Asked: Hello, what is the normal range of this kit?
Reply
M*****aSubmitted [ Aug 25 2022 ]
Asked: Does the tissue sample need to measure protein concentrations?
Reply
adminSubmitted [ Aug 25 2022 ]
Answered: No, there is no need to detect the protein concentration. You can calculate through wet weight directly.
M*****aSubmitted [ Aug 17 2022 ]
Asked: Can the kits be used for cell sample? If yes, How many cells do we need?
Reply
adminSubmitted [ Aug 17 2022 ]
Answered: It is suitable for cell sample and the amout of the cell is at least 2*10^6.
M*****aSubmitted [ Aug 15 2022 ]
Asked: Can the kits be used for cell sample? If yes, How many cells do we need?
Reply
adminSubmitted [ Aug 15 2022 ]
Answered: It is suitable for cell sample and the amout of the cell is at least 2*10^6.