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Total Oxidant Status (TOS) Colorimetric Assay Kit

  • Cat.No.:E-BC-K802-M

  • Detection instrument: Microplate reader(580 nm-590 nm,optimum wavelength: 590 nm)

To Purchase E-BC-K802-M

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Price: $150

Detection principle

Under acid conditions, the oxidizing material in the sample can oxidize Fe2+ to Fe3+, which binds highly with xylenol orange to produce a blue-purple complex. When the pH of solution is in the range of 2-3, its maximum absorption wavelength is around 590 nm, and the color depth is proportional to the content of oxidation substances in a certain concentration and a certain time, so as to indirectly calculate the total oxidation state of the sample.

Performance characteristics

Synonyms TOS
Sample type tissue, serum and other liquid samples
Sensitivity 2.5 μmol H2O2 Equiv./L
Detection range 2.5-100 μmol H2O2 Equiv./L
Detection method Colorimetric method
Assay type Quantitative
Assay time 30 min
Precision Average inter-assay CV: 3.5%Average intra-assay CV: 2.3%
Other instruments required Micropipettor, 37℃ incubator.
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 3 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (2.5-100 μmol H2O2 Equiv./L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum


Porcine serum


Horse serum


Cynomolgus macaques serum


10% Rat liver tissue homogenate


10% Rat kidney tissue homogenate


10% Rat lung tissue homogenate


10% Rat spleen tissue homogenate


Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).


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