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Total Oxidant Status (TOS) Colorimetric Assay Kit

Cat.No.:E-BC-K802-M
Detection instrument: Microplate reader(580 nm-590 nm,optimum wavelength: 590 nm)

Detection method: Colorimetric method

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Detection principle

Under acid conditions, the oxidizing material in the sample can oxidize Fe2+ to Fe3+, which binds highly with xylenol orange to produce a blue-purple complex. When the pH of solution is in the range of 2-3, its maximum absorption wavelength is around 590 nm, and the color depth is proportional to the content of oxidation substances in a certain concentration and a certain time, so as to indirectly calculate the total oxidation state of the sample.

Performance characteristics

Synonyms	TOS
Sample type	tissue, serum and other liquid samples
Sensitivity	2.5 μmol H2O2 Equiv./L
Detection range	2.5-100 μmol H2O2 Equiv./L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	30 min
Precision	Average inter-assay CV: 3.5%Average intra-assay CV: 2.3%
Other instruments required	Micropipettor, 37℃ incubator.
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	3 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (2.5-100 μmol H₂O₂ Equiv./L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Porcine serum	1
Horse serum	1
Cynomolgus macaques serum	1
10% Rat liver tissue homogenate	1
10% Rat kidney tissue homogenate	1
10% Rat lung tissue homogenate	1
10% Rat spleen tissue homogenate	1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Ralated product

Cat Number	Product Name
E-BC-K138-F	Reactive Oxygen Species (ROS) Fluorometric Assay Kit
E-BC-K801-M	Total Antioxidant Status (TAS) Colorimetric Assay Kit

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

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