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Total Oxidant Status (TOS) Colorimetric Assay Kit

Cat.No.:E-BC-K802-M
Detection instrument: Microplate reader(580 nm-590 nm,optimum wavelength: 590 nm)

Detection method: Colorimetric method

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Detection principle

Under acid conditions, the oxidizing material in the sample can oxidize Fe2+ to Fe3+, which binds highly with xylenol orange to produce a blue-purple complex. When the pH of solution is in the range of 2-3, its maximum absorption wavelength is around 590 nm, and the color depth is proportional to the content of oxidation substances in a certain concentration and a certain time, so as to indirectly calculate the total oxidation state of the sample.

Performance characteristics

Synonyms	TOS
Sample type	cell, tissue, serum and other liquid samples
Sensitivity	2.5 μmol H2O2 Equiv./L
Detection range	2.5-100 μmol H2O2 Equiv./L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	30 min
Precision	Average inter-assay CV: 3.500%Average intra-assay CV: 2.300%
Other instruments required	Micropipettor, 37℃ incubator.
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months

Images

Different subscript letters indicate significant differences among the groups. (P<0.05)

X Zhao et al investigate the function of quercetin for reducing ethanol-induced hepatocyte pyroptosis. Total oxidants status (TOS) of human L02 cell was determined using TOS colorimetric assay kit (E-BC-K802-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (2.5-100 μmol H₂O₂ Equiv./L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Porcine serum	1
Horse serum	1
Cynomolgus macaques serum	1
10% Rat liver tissue homogenate	1
10% Rat kidney tissue homogenate	1
10% Rat lung tissue homogenate	1
10% Rat spleen tissue homogenate	1
0.612×10^6 Molt-4 cells	1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Oxidative Medicine and Cellular Longevity (2022) IF: 7.31

Quercetin Protects Ethanol-Induced Hepatocyte Pyroptosis via Scavenging Mitochondrial ROS and Promoting PGC-1α-Regulated Mitochondrial Homeostasis in L02 Cells

DOI: 10.1155/2022/4591134
	Sample: L02 cell

Biology-Basel (2021) IF: 5.079

Cellular Stress Pathways Are Linked to Acetamiprid-Induced Apoptosis in SH-SY5Y Neural Cells

DOI: 10.3390/biology10090820
	Sample: SH-SY5Y cell

JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2022) IF: 3.652

Zoledronic acid-induced oxidative damage and endoplasmic reticulum stress-mediated apoptosis in human embryonic kidney (HEK-293) cells

DOI: 10.1002/jbt.23083
PMID: 35587103	Sample: HEK-293 cell

BIOLOGICAL TRACE ELEMENT RESEARCH (2022) IF: 3.738

Selenoprotein Gene mRNA Expression Evaluation During Renal Ischemia–Reperfusion Injury in Rats and Ebselen Intervention Effects

DOI: 10.1007/s12011-022-03275-7
	Sample: kidney

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Reviews/Q&A

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Reviews
Q&A

B********eSubmitted [ Aug 17 2023 ]

Asked: We preapered the standardı solution using the normal saline however our sample and standards shows different colours(blue and purple). Is it a normal? Normally, the sample colours have to be range of the standard colours.

adminSubmitted [ Aug 17 2023 ]

Answered:

adminSubmitted [ Aug 17 2023 ]

Answered: This is a normal phenomenon, the higher concentration in the standard solution is purple, and the color of the sample is related to the TOS content in the sample, which may be blue or purple.

D**************nSubmitted [ Jul 07 2023 ]

Asked: What is the cost in Indian rupees?

adminSubmitted [ Jul 07 2023 ]

Answered: Hello Dr seema chawhan,thanks for your support.We will reply you by E-mail later.

E*oSubmitted [ Nov 24 2022 ]

Asked: Hello, what is the normal range of this kit?

adminSubmitted [ Nov 24 2022 ]

Answered: Hi Eco, The normal range for E-BC-K802-M of human serum is 33.92μmol H2O2 Equiv./L, rat serum is 97.62μmol H2O2 Equiv./L, and for mouse is 61.04μmol H2O2 Equiv./L, just for reference. If any further questions, pls feel free to contact us.

C*****BSubmitted [ Oct 27 2022 ]

Asked: hi, I\'m trying to see the wavelength through Elisa, do you have the necessary equipment for additional experiments?

adminSubmitted [ Oct 27 2022 ]

Answered: Hi Chae SB, For Instruments, Microplatereader(580-590 nm.optimum wavelength: 590nm). Micropipettor37℃ incubator. Best regards.

C*****BSubmitted [ Oct 27 2022 ]

Asked: Hi, I\'m trying to see the wavelength through Elisa, do you have the necessary equipment for additional experiments?

adminSubmitted [ Oct 27 2022 ]

Answered: Hi Chae SB, For Instruments, Microplatereader(580-590 nm.optimum wavelength: 590nm). Micropipettor37℃ incubator. Best regards.

M*****aSubmitted [ Sep 23 2022 ]

Asked: What chromogen is used in TOS kit?

adminSubmitted [ Sep 23 2022 ]

Answered: The chromogen is xylenol orange.

M*****aSubmitted [ Sep 06 2022 ]

Asked: Hi, I would like to confirm with you that can E-BC-K802-M be used for rat samples?

adminSubmitted [ Sep 06 2022 ]

Answered: Yes, of course. The kit can be used for the determination of total oxidant status (TOS) in animal tissue, serum and plasma samples

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