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Current Position:Home>>Kits>>Metabolism Assays>> Total Superoxide Dismutase (T-SOD) Activity Assay Kit (WST-1 Method)

Total Superoxide Dismutase (T-SOD) Activity Assay Kit (WST-1 Method)

Citations(120)

  • Cat.No.:E-BC-K020-M

  • Detection instrument: Microplate reader(440-460 nm, optimum wavelength: 450 nm)

Manual MSDS

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  • 48T
  • 500Assays
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Detection principle

The activity of SOD was measured by WST-1 method in this kit and the principles of the WST-1 is as follows. Xanthine Oxidase (XO) can catalyze WST-1 react with O2.- to generate a water-soluble formazan dye. SOD can catalyze the disproportionation of superoxide anions, so the reaction can be inhibited by SOD, and the activity of SOD is negatively correlated with the amount of formazan dye. Therefore, the activity of SOD can be determined by the colorimetric analysis of WST-1 products.

Performance characteristics

Synonyms T-SOD
Sample type Serum,plasma,hydrothorax,ascites,urine,cells,tissue
Sensitivity 0.2 U/mL
Detection range 0.2 -14.4 U/mL
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 30 min
Precision Average inter-assay CV: 3.700%Average intra-assay CV: 2.900%
Other instruments required Micropipettor, Multichannel pipettor, Vortex mixer, Incubator
Other reagents required Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4)
Storage Reagent 3: -20℃, others: 2-8℃
Valid period 12 months

Images

The activity of SOD increased at the beginning of the UV exposure until 80 mJ/cm2, then decreased with the UV fluence reached 120 mJ/cm2. The induced SOD activity was higher in the UV/Cl2 process (purple) compared with that of the UV alone process.

Q Wan et al investigate the efficiency and mechanism of fungal spore disinfection using UV-LEDs irradiation and chlorine. Total superoxide dismutase (T-SOD) activity of fungal spore was determined using T-SOD activity assay kit (E-BC-K020-M).

Dilution of sample

The optimal sampling volume are different for different species, the SOD also are different for different samples. It is recommended to take 2~3 samples to do a pre-experiment, diluting a series of diluent and determine the dilution factor when the SOD inhibition ratio is 25%~65% (the optimal inhibition ratio is the range of 40%~60%.) before formal experiment.

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

3-5

Rat serum

20-30

Urine

1

Human hydrothorax

2

Cell culture supernatant

2-3

10% Rat liver tissue homogenate

340-370

10% Rat heart tissue homogenate

80-100

10% Rat kidney tissue homogenate

100-120

10% Rat brain tissue homogenate

50-100

10% Plant tissue homogenate

5-10

HepG2 cells (3 mgprot/mL)

30-40

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Citations

  1. Biomolecules (2019) IF: 4.694
    Curcumin Attenuates Lead-Induced Cerebellar Toxicity in Rats via Chelating Activity and Inhibition of Oxidative Stress

    DOI: 10.3390/biom9090453

    PMID: 31489882

    		 Sample: Tissue Homogenate
  2. JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 5.195
    Antidiabetic effects of Psidium x durbanensis Baijnath & Ramcharun ined. (Myrtaceae) leaf extract on streptozotocin-induced diabetes in rats

    DOI: 10.1016/j.jep.2022.115542

    PMID: 35842177

    		 Sample: Liver
  3. Frontiers in Pharmacology (2022) IF: 5.988
    Anti-arthritic and toxicological evaluation of ethanolic extract of Alternanthera bettzickiana in rats.

    DOI: 10.3389/fphar.2022.1002037
    Sample: Serum
  4. Frontiers in Molecular Biosciences (2022) IF: 6.113
    Biochemical profiling of metabolomics in heavy metal-intoxicated impaired metabolism and its amelioration using plant-based bioactive compound

    DOI: 10.3389/fmolb.2022.1029729

    PMID: 36330218

    		 Sample: Liver
  5. LIVER TRANSPLANTATION (2022) IF: 5.799
    Cerium oxide nanoparticles administration during machine perfusion of discarded human livers: a pilot study

    DOI: 10.1002/lt.26421

    PMID: 35100468

    		 Sample: Liver
  6. Journal of Functional Foods (2022) IF: 5.223
    Clinical Investigation of French Maritime Pine Bark Extract on Attention-Deficit Hyperactivity Disorder as compared to Methylphenidate and Placebo: Part 2: Oxidative Stress and Immunological Modulation

    DOI: 10.1016/j.jff.2022.105247
    Sample: Plasma
  7. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
    Palm Oil Derived Tocotrienol-Rich Fraction Attenuates Vascular Dementia in Type 2 Diabetic Rats

    DOI: 10.3390/ijms232113531
    Sample: Brain
  8. ARYA Atherosclerosis (2021)
    The effect of subchronic cigarette smoke exposure on oxidative stress parameters and endothelial nitric oxide synthase in a rat aorta

    DOI: 10.22122/arya.v17i0.2150
    Sample: Aortas
  9. Journal of Toxicology (2020)
    Styrene Oxide Caused Cell Cycle Arrest and Abolished Myogenic Differentiation of C2C12 Myoblasts

    DOI: 10.1155/2020/1807126
    Sample: C2C12 Cell
  10. Journal of Nutrition and Metabolism (2022)
    Protective Effect of High-Intensity Interval Training (HIIT) and Moderate-Intensity Continuous Training (MICT) against Vascular Dysfunction in Hyperglycemic Rats

    DOI: 10.1155/2022/5631488
    Sample: Aortic Tissue
  11. African Journal of Pharmacy and Pharmacology (2019)
    Hepatoprotective and antioxidant activities of Pterocarpus santalinoides methanol leaf extract

    DOI: 10.5897/AJPP2019.5090
    Sample: Serum
  12. Toxicology Reports (2019)
    Elevated reproductive toxicity effects of diclofenac after withdrawal: Investigation of the therapeutic role of melatonin

    DOI: 10.1016/j.toxrep.2019.06.009
    Sample: Plasma
  13. Advances in Traditional Medicine (2020)
    Anti-hypernociceptive effects of methanol extract of Boswellia dalzielii on STZ-induced diabetic neuropathic pain

    DOI: 10.1007/s13596-019-00411-y
    Sample: Serum,Sciatic Nerves
  14. Brazilian Journal of Pharmaceutical Sciences (2022) IF: 1.214
    Stingless bee propolis, metformin, and their combination alleviate diabetic cardiomyopathy

    DOI: 10.1590/s2175-97902022e19652
    Sample: Heart Tissue
  15. HORTSCIENCE (2021) IF: 1.455
    Effects of Salt Stress on Photosynthetic Fluorescence Characteristics, Antioxidant System, and Osmoregulation of Coreopsis tinctoria Nutt.

    DOI: 10.21273/HORTSCI15956-21
    Sample: Seed
  16. BRAZILIAN JOURNAL OF BIOLOGY (2023) IF: 1.651
    Neuroprotective potential of lignan-rich fraction of Piper cubeba L. by improving antioxidant capacity in the rat's brain

    DOI: 10.1590/1519-6984.266573
    Sample: Brain
  17. Journal of Plant Interactions (2018) IF: 1.655
    Brevibacillus sp. promotes maize root colonization by Acaulospora tuberculata and the alteration of associated plant protein responses

    DOI: 10.1080/17429145.2018.1547844
    Sample: Maize Roots
  18. JOURNAL OF FOOD BIOCHEMISTRY (2020) IF: 1.662
    Biochemical and histopathological assessment of liver in a rat model of metabolic syndrome induced by high‐carbohydrate high‐fat diet

    DOI: 10.1111/jfbc.13371
    Sample: Tissue Homogenate
  19. JOURNAL OF INTERNATIONAL MEDICAL RESEARCH (2021) IF: 1.671
    Effects of shikonin on the development of ovarian follicles and female germline stem cells:

    DOI: 10.1177/03000605211029461
    Sample: Blood
  20. Cutaneous and Ocular Toxicology (2021) IF: 1.82
    Effects of Achillea millefolium on cisplatin induced ocular toxicity: an experimental study

    DOI: 10.1080/15569527.2021.1919137

    PMID: 34180746

    		 Sample: Ocular
  21. BIOMEDICAL AND ENVIRONMENTAL SCIENCES (2019) IF: 1.917
    Effects of Simulated Heat Wave and Ozone on High Fat Diet ApoE Deficient Mice

    DOI: 10.3967/bes2018.101
    Sample: Plasma,Tissue Homogenate
  22. Canadian Journal of Gastroenterology and Hepatology (2020) IF: 2.055
    Bredemolic Acid Ameliorates Selected Liver Function Biomarkers in a Diet-Induced Prediabetic Rat Model

    DOI: 10.1155/2020/2475301

    PMID: 32149046

    		 Sample: Tissue Homogenate
  23. 3 Biotech (2021) IF: 2.406
    Rapamycin inhibits LOC102553434-mediated pyroptosis to improve lung injury induced by limb ischemia–reperfusion

    DOI: 10.1007/s13205-021-02708-9
    Sample: Lung
  24. VETERINARY RESEARCH COMMUNICATIONS (2022) IF: 2.459
    Infection of West African dwarf rams with Trypanosoma brucei brucei and Trypanosoma congolense significantly alter serum electrolytes, redox balance, sperm parameters, and gonadal morphology

    DOI: 10.1007/s11259-022-09921-8

    PMID: 35389159

    		 Sample: Serum
  25. Translational Andrology and Urology (2022) IF: 2.479
    Astragaloside IV protects against ischemia/reperfusion (I/R)-induced kidney injury based on the Keap1-Nrf2/ARE signaling pathway.

    DOI: 10.21037/tau-22-505
    Sample: Kidney Tissue
  26. PHYSIOLOGY & BEHAVIOR (2018) IF: 2.517
    Resveratrol prevents cognitive deficits by attenuating oxidative damage and inflammation in rat model of streptozotocin diabetes induced vascular dementia

    DOI: 10.1016/j.physbeh.2018.12.012

    PMID: 30550811

    		 Sample: Serum,Tissue Homogenate
  27. JOURNAL OF MOLECULAR HISTOLOGY (2020) IF: 2.531
    Oxidative stress induced pyroptosis leads to osteogenic dysfunction of MG63 cells

    DOI: 10.1007/s10735-020-09874-9

    PMID: 32356234

    		 Sample: Cell Culture Supernatant
  28. ALCOHOL (2022) IF: 2.558
    Vinpocetine a PDE1 modulator regulates markers of cerebral health, inflammation, and oxidative stress in a rat model of prenatal alcohol-induced experimental attention deficit hyperactivity disorder

    DOI: 10.1016/j.alcohol.2022.08.005
    Sample: Brain
  29. JOURNAL OF MOLECULAR HISTOLOGY (2021) IF: 2.611
    MiRNA-182-5p aggravates experimental ulcerative colitis via sponging Claudin-2

    DOI: 10.1007/s10735-021-10021-1

    PMID: 34623552

    		 Sample: Colonic Tissue
  30. Dose-Response (2022) IF: 2.623
    Pharmacological Role of Capparis decidua (Forssk.) Edgew in Preventing Cyclophosphamide-induced Myelosuppression and Modulating Innate and Adaptive Immune Response:

    DOI: 10.1177/15593258221123672
    Sample: Serum
  31. Evidence-based Complementary and Alternative Medicine (2021) IF: 2.63
    Zanthoxylum bungeanum Seed Oil Attenuates LPS-Induced BEAS-2B Cell Activation and Inflammation by Inhibiting the TLR4/MyD88/NF-κB Signaling Pathway

    DOI: 10.1155/2021/2073296
    Sample: Beas-2B Cell
  32. Evidence-based Complementary and Alternative Medicine (2022) IF: 2.63
    Etlingera elatior Flower Aqueous Extract Protects against Oxidative Stress-Induced Nephropathy in a Rat Model of Type 2 Diabetes

    DOI: 10.1155/2022/2814196

    PMID: 35502173

    		 Sample: Plasma
  33. Sleep and Breathing (2023) IF: 2.655
    The role of ferroptosis in chronic intermittent hypoxia-induced cognitive impairment

    DOI: 10.1007/s11325-022-02760-6
    Sample: Hippocampal Tissue
  34. Journal of Stroke & Cerebrovascular Diseases (2022) IF: 2.677
    Circ_0101874 overexpression strengthens PDE4D expression by targeting miR-335-5p to promote neuronal injury in ischemic stroke

    DOI: 10.1016/j.jstrokecerebrovasdis.2022.106817

    PMID: 36252429

    		 Sample: Sk-N-Sh Cells
  35. Toxicology Research (2022) IF: 2.68
    Glutathione attenuated lambda-cyhalothrin-induced alteration of serum total cholesterol concentration and oxidative stress parameters in rats

    DOI: 10.1093/toxres/tfac080
    Sample: Serum
  36. JOURNAL OF FOOD BIOCHEMISTRY (2022) IF: 2.72
    Watermelon (Citrullus lanatus) leaf extract attenuates biochemical and histological parameters in high-fat diet/streptozotocin-induced diabetic rats

    DOI: 10.1111/jfbc.14058

    PMID: 34981526

    		 Sample: Serum
  37. JOURNAL OF FOOD BIOCHEMISTRY (2022) IF: 2.72
    Effect of coconut water and milk on heat stress-induced gastrointestinal tract dysmotility in rats: Role of oxidative stress and inflammatory response

    DOI: 10.1111/jfbc.14129

    PMID: 35298033

    		 Sample: Gastric,Jejunal, Colonic
  38. JOURNAL OF FOOD BIOCHEMISTRY (2021) IF: 2.72
    Antioxidant and antithrombotic effects of green mussels (Perna canaliculus) in rats

    DOI: 10.1111/jfbc.13865

    PMID: 34263474

    		 Sample: Liver，Kidney
  39. JOURNAL OF FOOD BIOCHEMISTRY (2021) IF: 2.72
    A standardized black pepper seed extract containing β-caryophyllene improves cognitive function in scopolamine-induced amnesia model mice via regulation of brain-derived neurotrophic factor and MAPK proteins

    DOI: 10.1111/jfbc.13994

    PMID: 34778972

    		 Sample: Brain
  40. Animals (2021) IF: 2.752
    High Granulocyte-Macrophage Colony Stimulating Factor to Interleukin 10 Ratio and Marked Antioxidant Enzyme Activities Predominate in Symptomatic Cattle Naturally Infected with Candidatus Mycoplasma haemobos, Theileria orientalis, Theileria sinensis and T

    DOI: 10.3390/ani11082235
    Sample: Serum
  41. ACS Omega (2020) IF: 2.87
    Antiarthritic Potential of Comprehensively Standardized Extract of Alternanthera bettzickiana: In Vitro and In Vivo Studies

    DOI: 10.1021/acsomega.0c01670

    PMID: 32803042

    		 Sample: Serum
  42. Journal of Diabetes Research (2018) IF: 2.885
    The Effects of Momordica balsamina Methanolic Extract on Kidney Function in STZ-Induced Diabetic Rats: Effects on Selected Metabolic Markers

    DOI: 10.1155/2018/7341242
    Sample: Kidney Tissues
  43. Analytical Methods (2022) IF: 2.896
    Evaluate the Effect of nitrate and chloride on Cd(II)-Induced Single Cell Oxidative Stress by Scanning Electrochemical Microscopy

    DOI: 10.1039/D2AY00495J
    Sample: Mcf-7 Cell
  44. HUMAN & EXPERIMENTAL TOXICOLOGY (2021) IF: 2.903
    Scutellarin alleviates type 2 diabetes (HFD/low dose STZ)-induced cardiac injury through modulation of oxidative stress, inflammation, apoptosis and fibrosis in mice:

    DOI: 10.1177/09603271211045948
    Sample: Heart
  45. THERIOGENOLOGY (2023) IF: 2.923
    Seasonal variations in the metabolomic profile of the ovarian follicle components in Italian Mediterranean Buffaloes

    DOI: 10.1016/j.theriogenology.2023.02.022
    Sample: Follicular Fluid
  46. CURRENT ISSUES IN MOLECULAR BIOLOGY (2023) IF: 2.976
    Chemopreventive Effects of Onosma mutabilis against Azoxymethane-Induced Colon Cancer in Rats via Amendment of Bax/Bcl-2 and NF-κB Signaling Pathways

    DOI: 10.3390/cimb45020057
    Sample: Colonic Tissue
  47. PeerJ (2021) IF: 2.984
    Physiological changes and transcript identification in Coreopsis tinctoria Nutt. in early stages of salt stress

    DOI: 10.7717/peerj.11888

    PMID: 34434650

    		 Sample: Seedling
  48. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2022) IF: 3.094
    Native SodB Overexpression of Synechocystis sp. PCC 6803 Improves Cell Growth Under Alcohol Stresses Whereas Its Gpx2 Overexpression Impacts on Growth Recovery from Alcohol Stressors

    DOI: 10.1007/s12010-022-04061-w

    PMID: 35819692

    		 Sample: Pcc 6803 Strains
  49. Frontiers in Bioscience-Landmark (2022) IF: 3.115
    SIRT3 Enhances the Protective Role of Propofol in Postoperative Cognitive Dysfunction via Activating Autophagy Mediated by AMPK/mTOR Pathway

    DOI: 10.31083/j.fbl2711303
    Sample: Hippocampal Tissue,Primary Neurons
  50. EUROPEAN JOURNAL OF NEUROSCIENCE (2020) IF: 3.115
    Neuroprotective effects of brain‐derived neurotrophic factor against amyloid beta 1‐40‐induced retinal and optic nerve damage

    DOI: 10.1111/ejn.14662
    Sample: Tissue Homogenate
  51. IMMUNOBIOLOGY (2022) IF: 3.144
    PM2.5 induced lung injury through upregulating ROS-Dependent NLRP3 Inflammasome-Mediated Pyroptosis

    DOI: 10.1016/j.imbio.2022.152207

    PMID: 35344845

    		 Sample: Raw264.7 Cell
  52. Diabetes Metabolic Syndrome and Obesity-Targets and Therapy (2022) IF: 3.168
    ViphyllinTM, a Standardized Black Pepper Extract Exerts Antihyperglycemic Effect and Improves Sciatic Nerve Conduction in High Fat Diet/Streptozotocin-Induced Diabetic Model Rats

    DOI: 10.2147/DMSO.S366609
    Sample: Sciatic Nerve Tissue
  53. EUROPEAN JOURNAL OF PHARMACOLOGY (2019) IF: 3.17
    Intraocular pressure-lowering effects of imidazo[1,2-a]- and pyrimido[1,2-a]benzimidazole compounds in rats with dexamethasone-induced ocular hypertension

    DOI: 10.1016/j.ejphar.2019.01.059
    Sample: Tissue Homogenate
  54. Animals (2023) IF: 3.231
    The Effects of Dietary Spirulina platensisis on Physiological Responses of Broiler Chickens Exposed to Endotoxin Stress

    DOI: 10.3390/ani13030363
    Sample: Plasma
  55. Biomed Research International (2022) IF: 3.246
    Red Raspberry Extract Decreases Depression-Like Behavior in Rats by Modulating Neuroinflammation and Oxidative Stress

    DOI: 10.1155/2022/9943598

    PMID: 35818443

    		 Sample: Hippocampal Tissue
  56. NEUROPEPTIDES (2021) IF: 3.286
    Dynorphin A (1–8) inhibits oxidative stress and apoptosis in MCAO rats, affording neuroprotection through NMDA receptor and κ-opioid receptor channels

    DOI: 10.1016/j.npep.2021.102182
    Sample: Serum
  57. ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY (2020) IF: 3.292
    Chronic exposure of bisphenol A impairs carbohydrate and lipid metabolism by altering corresponding enzymatic and metabolic pathways

    DOI: 10.1016/j.etap.2020.103387

    PMID: 32339907

    		 Sample: Serum
  58. NEUROTOXICITY RESEARCH (2019) IF: 3.311
    Antiproliferative Effect of Elastin-Derived Peptide VGVAPG on SH-SY5Y Neuroblastoma Cells

    DOI: 10.1007/s12640-019-00040-y

    PMID: 31161598

    		 Sample: Sh-Sy5Y Cells
  59. ANIMAL FEED SCIENCE AND TECHNOLOGY (2022) IF: 3.313
    Housefly maggot meal complement soybean meal in a fish-free diet for hybrid catfish (Clarias gariepinus ♀ x Heterobranchus longifilis ♂): Effect on growth, body composition, blood biochemistry and antioxidant enzyme activity

    DOI: 10.1016/j.anifeedsci.2022.115543
    Sample: Serum
  60. Processes (2022) IF: 3.352
    Gastroprotective, Biochemical, and Acute Toxicity Effects of Papaver decaisnei against Ethanol-Induced Gastric Ulcers in Rats

    DOI: 10.3390/pr10101985
    Sample: Gastric Tissue
  61. Processes (2022) IF: 3.352
    Gastroprophylactic Effects of p-Cymene in Ethanol-Induced Gastric Ulcer in Rats

    DOI: 10.3390/pr10071314
    Sample: Stomach
  62. International Journal of Environmental Research and Public Health (2021) IF: 3.39
    Polystyrene Microplastics Exposure: An Insight into Multiple Organ Histological Alterations, Oxidative Stress and Neurotoxicity in Javanese Medaka Fish (Oryzias javanicus Bleeker, 1854)

    DOI: 10.3390/ijerph18189449
    Sample: Intestine,Brain
  63. Biomed Research International (2022) IF: 3.411
    Bushen Huoxue Formula Modulates Autophagic Flux and Inhibits Apoptosis to Protect Nucleus Pulposus Cells by Restoring the AMPK/SIRT1 Pathway

    DOI: 10.1155/2022/8929448

    PMID: 35669720

    		 Sample: Np Cell
  64. Biomed Research International (2021) IF: 3.411
    Antioxidant Activity Derived from Marine Green-Lipped Mussel Perna canaliculus Extracts in Mice

    DOI: 10.1155/2021/1622270

    PMID: 34409099

    		 Sample: Liver,Kidney,Cardiac
  65. PHARMACEUTICAL BIOLOGY (2022) IF: 3.503
    Combination of puerarin and tanshinone IIA alleviates ischaemic stroke injury in rats via activating the Nrf2/ARE signalling pathway

    DOI: 10.1080/13880209.2022.2070221

    PMID: 35635784

    		 Sample: Brain Tissue
  66. ACS Omega (2022) IF: 3.512
    Sarcococca saligna Hydroalcoholic Extract Ameliorates Arthritis in Complete Freund’s Adjuvant-Induced Arthritic Rats via Modulation of Inflammatory Biomarkers and Suppression of Oxidative Stress Markers

    DOI: 10.1021/acsomega.2c00619
    Sample: Serum
  67. Food Science & Nutrition (2022) IF: 3.553
    Curcumin-loaded cockle shell-derived calcium carbonate nanoparticles ameliorates lead-induced neurotoxicity in rats via attenuation of oxidative stress

    DOI: 10.1002/fsn3.3096
    Sample: Cerebellum,Cerebrum
  68. METABOLIC BRAIN DISEASE (2022) IF: 3.584
    LncRNA ZFAS1 regulates the hippocampal neurons injury in epilepsy through the miR-15a-5p/OXSR1/NF-κB pathway

    DOI: 10.1007/s11011-022-01013-5

    PMID: 35751788

    		 Sample: Hippocampal Neurons
  69. JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2020) IF: 3.606
    Ameliorative effect of curcumin on lead‐induced hematological and hepatorenal toxicity in a rat model

    DOI: 10.1002/jbt.22483
    Sample: Serum
  70. APPLIED CLAY SCIENCE (2018) IF: 3.641
    Determination of half maximal inhibitory concentration of CaAl layered double hydroxide on cancer cells and its role in the apoptotic pathway

    DOI: 10.1016/j.clay.2018.10.013
    Sample: Hct 116 Cells
  71. JOURNAL OF FOOD BIOCHEMISTRY (2022) IF: 3.654
    Viphyllin™, a standardized extract from black pepper seeds, mitigates intestinal inflammation, oxidative stress, and anxiety-like behavior in DSS-induced colitis mice

    DOI: 10.1111/jfbc.14306

    PMID: 35766031

    		 Sample: Colon Tissue,Brain Tissue
  72. INTERNATIONAL IMMUNOPHARMACOLOGY (2020) IF: 3.943
    Astragaloside IV alleviates PM2.5-induced lung injury in rats by modulating TLR4/MyD88/NF-κB signalling pathway

    DOI: 10.1016/j.intimp.2020.107290

    PMID: 33383446

    		 Sample: Lung Tissue
  73. CELLULAR SIGNALLING (2020) IF: 3.968
    Clostridium perfringens phospholipase C impairs innate immune response by inducing integrated stress response and mitochondrial-induced epigenetic modifications

    DOI: 10.1016/j.cellsig.2020.109776

    PMID: 32916276

    		 Sample: Cell Sample
  74. CELLULAR SIGNALLING (2020) IF: 3.968
    Circular RNA circ_DROSHA alleviates the neural damage in a cell model of temporal lobe epilepsy through regulating miR-106b-5p/MEF2C axis

    DOI: 10.1016/j.cellsig.2020.109901

    PMID: 33370579

    		 Sample: Sk-N-Sh Cell,Imr-32 Cell
  75. JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY (2021) IF: 3.981
    Using PCG-Arginine nanoparticle mediated intranasal delivery of dynorphin A (1–8) to improve neuroprotection in MCAO rats

    DOI: 10.1016/j.jddst.2021.103059
    Sample: Serum
  76. SAUDI JOURNAL OF BIOLOGICAL SCIENCES (2022) IF: 4.052
    Evaluation of testicular torsion management in Ogbomoso, South-Western Nigeria and surgical detorsion-augmented treatment with phytochemical fractions of Corchorus olitorius leaf in experimental rats

    DOI: 10.1016/j.sjbs.2022.103495
    Sample: Testicular Tissue
  77. BIOLOGICAL TRACE ELEMENT RESEARCH (2022) IF: 4.081
    Selenium Supplementation Protects Against Arsenic-Trioxide-Induced Cardiotoxicity Via Reducing Oxidative Stress and Inflammation Through Increasing NAD+ Pool

    DOI: 10.1007/s12011-022-03478-y
    Sample: Heart Tissue
  78. SAUDI JOURNAL OF BIOLOGICAL SCIENCES (2021) IF: 4.219
    Edible bird’s nest protects histomorphology of rat’s uterus against cadmium (Cd) toxicity through a reduction of Cd deposition and enhanced antioxidant activity

    DOI: 10.1016/j.sjbs.2021.08.012
    Sample: Plasma
  79. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2022) IF: 4.223
    Prenatal exposure to environmental pro-oxidants induces mitochondria-mediated epigenetic changes: a cross-sectional pilot study

    DOI: 10.1007/s11356-022-21059-3

    PMID: 35633452

    		 Sample: Plasma
  80. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2022) IF: 4.223
    Differential neuroprotective effect of curcuminoid formulations in aluminum chloride–induced Alzheimer’s disease

    DOI: 10.1007/s11356-022-20593-4
    Sample: Brain Tissue
  81. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2022) IF: 4.223
    Biochemical profiling of lead-intoxicated impaired lipid metabolism and its amelioration using plant-based bioactive compound

    DOI: 10.1007/s11356-022-20069-5

    PMID: 35420345

    		 Sample: Serum
  82. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2021) IF: 4.223
    Biochemical investigation of human exposure to aflatoxin M1 and its association with risk factors of diabetes mellitus

    DOI: 10.1007/s11356-021-14871-w

    PMID: 34216342

    		 Sample: Serum
  83. Journal of Diabetes Investigation (2021) IF: 4.232
    Diet-induced prediabetes: Effects on the activity of the renin-angiotensin-aldosterone system (RAAS) in selected organs

    DOI: 10.1111/jdi.13690
    Sample: Skeletal Muscle,Heart,Pancreas
  84. Food Bioscience (2021) IF: 4.24
    Antiaging activity of polyphenol rich Calophyllum inophyllum L. fruit extract in Saccharomyces cerevisiae BY611 yeast cells

    DOI: 10.1016/j.fbio.2021.101208
    Sample: By611 Cells
  85. Foods (2022) IF: 4.35
    Protective Effects of a Novel Probiotic Bifidobacterium pseudolongum on the Intestinal Barrier of Colitis Mice via Modulating the Pparγ/STAT3 Pathway and Intestinal Microbiota

    DOI: 10.3390/foods11111551
    Sample: Colon Tissue
  86. JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 4.36
    Sub-chronic oral toxicity evaluation of herbo-metallic formulation Arshakuthar rasa in rats

    DOI: 10.1016/j.jep.2022.115306

    PMID: 35443217

    		 Sample: Serum
  87. JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 4.36
    Anti-inflammatory and anti-apoptotic effects of Shaoyao decoction on X-ray radiation-induced enteritis of C57BL/6 mice

    DOI: 10.1016/j.jep.2022.115158

    PMID: 35245630

    		 Sample: Serum
  88. Scientific Reports (2022) IF: 4.38
    Molecular mechanisms associated with microbial biostimulant-mediated growth enhancement, priming and drought stress tolerance in maize plants

    DOI: 10.1038/s41598-022-14570-7

    PMID: 35729338

    		 Sample: Leaf
  89. Scientific Reports (2021) IF: 4.38
    Mesna ameliorates acute lung injury induced by intestinal ischemia–reperfusion in rats

    DOI: 10.1038/s41598-021-92653-7
    Sample: Lung
  90. EUROPEAN JOURNAL OF PHARMACOLOGY (2021) IF: 4.432
    Glycyrrhizic acid ameliorates myocardial ischemia-reperfusion injury in rats through inhibiting endoplasmic reticulum stress

    DOI: 10.1016/j.ejphar.2021.174353

    PMID: 34274339

    		 Sample: Myocardial
  91. BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545
    2,3,5,4'-tetrahydroxystilbence-2-O-β-D-glucoside attenuates hepatic steatosis via IKKβ/NF-κB and Keap1-Nrf2 pathways in larval zebrafish

    DOI: 10.1016/j.biopha.2020.110138
    Sample: Tissue Homogenate
  92. JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY (2022) IF: 4.553
    Investigation of the protective effect of gel incorporating Eugenia jambolana leaf extract on 5-fluorouracil-induced oral mucositis: an animal study

    DOI: 10.1007/s00432-022-04065-3

    PMID: 35622166

    		 Sample: Cheek,Liver,Kidney
  93. PROCESS BIOCHEMISTRY (2022) IF: 4.885
    Vernodalin regulated the NF-κβp65 signaling in inflammation of lipopolysaccharide -induced sepsis rats

    DOI: 10.1016/j.procbio.2022.10.004
    Sample: Liver,Kidney,Lungs
  94. MOLECULES (2022) IF: 4.927
    Chemical Profile of Cyperus laevigatus and Its Protective Effects against Thioacetamide-Induced Hepatorenal Toxicity in Rats

    DOI: 10.3390/molecules27196470

    PMID: 36235007

    		 Sample: Plasma
  95. INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 4.932
    Gastroprotective effect of an active ingredients group of Lindera reflexa Hemsl. On Ethanol-Induced gastric ulcers in Rats: Involvement of VEGFR2/ERK and TLR-2/Myd88 signaling pathway

    DOI: 10.1016/j.intimp.2022.108673

    PMID: 35259712

    		 Sample: Stomach Tissues
  96. Antioxidants (2020) IF: 5.014
    Dietary Postbiotic Lactobacillus plantarum Improves Serum and Ruminal Antioxidant Activity and Upregulates Hepatic Antioxidant Enzymes and Ruminal Barrier Function in Post-Weaning Lambs

    DOI: 10.3390/antiox9030250
    Sample: Serum,Ruminal Fluid
  97. LIFE SCIENCES (2022) IF: 5.037
    Trimetazidine attenuates cyclophosphamide-induced cystitis by inhibiting TLR4-mediated NFκB signaling in mice

    DOI: 10.1016/j.lfs.2022.120590

    PMID: 35504331

    		 Sample: Bladder
  98. Food Bioscience (2022) IF: 5.318
    Ameliorative effects of monascin from red mold rice on alcoholic liver injury and intestinal microbiota dysbiosis in mice

    DOI: 10.1016/j.fbio.2022.102079
    Sample: Livers
  99. Food & Function (2021) IF: 5.396
    Alhagi honey polysaccharides attenuate intestinal injury and immune suppression in cyclophosphamide-induced mice

    DOI: 10.1039/D1FO01008E

    PMID: 34128029

    		 Sample: Small Intestinal
  100. INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 5.714
    The role of AMPK-Sirt1-autophagy pathway in the intestinal protection process by propofol against regional ischemia/reperfusion injury in rats

    DOI: 10.1016/j.intimp.2022.109114

    PMID: 35933747

    		 Sample: Intestinal Tissue,Serum
  101. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY (2022) IF: 5.79
    Mitigation of benznidazole toxicity and oxidative stress following ascorbic acid supplementation in an adult traveller with chronic indeterminate Chagas’ disease

    DOI: 10.1093/jac/dkac093
    Sample: Serum
  102. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
    Ameliorative Effects of a Rhenium (V) Compound with Uracil-Derived Ligand Markers Associated with Hyperglycaemia-Induced Renal Dysfunction in Diet-Induced Prediabetic Rats

    DOI: 10.3390/ijms232315400
    Sample: Kidney
  103. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2022) IF: 6.291
    GPX4 degradation via chaperone-mediated autophagy contributes to antimony-triggered neuronal ferroptosis

    DOI: 10.1016/j.ecoenv.2022.113413

    PMID: 35305351

    		 Sample: Pc12 Cell
  104. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2022) IF: 6.291
    Antibacterial effects of nano-decoction iron polysulfide in epididymitis and the systematic evaluation of its toxicity on the reproductive health of male mice

    DOI: 10.1016/j.ecoenv.2022.113184

    PMID: 35032726

    		 Sample: Testis
  105. Cell Death & Disease (2020) IF: 6.304
    Pyroptosis and ferroptosis induced by mixed lineage kinase 3 (MLK3) signaling in cardiomyocytes are essential for myocardial fibrosis in response to pressure overload

    DOI: 10.1038/s41419-020-02777-3
    Sample: Heart
  106. INTERNATIONAL JOURNAL OF PHARMACEUTICS (2022) IF: 6.51
    Carboxymethyl cellulose/sericin-based hydrogels with intrinsic antibacterial, antioxidant, and anti-inflammatory properties promote re-epithelization of diabetic wounds in rats

    DOI: 10.1016/j.ijpharm.2022.122328

    PMID: 36280221

    		 Sample: Skin Tissues
  107. BIOMEDICINE & PHARMACOTHERAPY (2021) IF: 6.53
    Lipoic acid (LA) dose-dependently protects bone losses in the mandible of rats during the development of osteopenia by inhibiting oxidative stress and promoting bone formation

    DOI: 10.1016/j.biopha.2021.112467

    PMID: 34891114

    		 Sample: Serum
  108. BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 6.53
    Gastroprotective effect of gallic acid against ethanol-induced gastric ulcer in rats: Involvement of the Nrf2/HO-1 signaling and anti-apoptosis role

    DOI: 10.1016/j.biopha.2020.110075

    PMID: 32179202

    		 Sample: Tissue Homogenate
  109. Oxidative Medicine and Cellular Longevity (2021) IF: 6.543
    Leonurine Ameliorates Oxidative Stress and Insufficient Angiogenesis by Regulating the PI3K/Akt-eNOS Signaling Pathway in H2O2-Induced HUVECs

    DOI: 10.1155/2021/9919466
    Sample: Huvecs
  110. LIFE SCIENCES (2022) IF: 6.78
    A bovine milk-derived peptide ameliorates alloxan-injured pancreatic β cells through IRS2/PI3K/Akt signaling

    DOI: 10.1016/j.lfs.2022.120907

    PMID: 36037870

    		 Sample: Serum
  111. SENSORS AND ACTUATORS B-CHEMICAL (2020) IF: 7.1
    A facile Pt-doped g-C3N4 photocatalytic biosensor for visual detection of superoxide dismutase in serum samples

    DOI: 10.1016/j.snb.2020.128238
    Sample: Serum
  112. Antioxidants (2022) IF: 7.675
    Selenoprotein F Knockout Caused Glucose Metabolism Disorder in Young Mice by Disrupting Redox Homeostasis

    DOI: 10.3390/antiox11112105

    PMID: 36358477

    		 Sample: Liver,Serum
  113. Antioxidants (2022) IF: 7.675
    Mangosteen Pericarp Extract Supplementation Boosts Antioxidant Status via Rebuilding Gut Microbiota to Attenuate Motor Deficit in 6-OHDA-Induced Parkinson’s Disease

    DOI: 10.3390/antiox11122396
    Sample: Serum
  114. SCIENCE OF THE TOTAL ENVIRONMENT (2021) IF: 7.963
    Polystyrene microplastics induce hepatotoxicity and disrupt lipid metabolism in the liver organoids

    DOI: 10.1016/j.scitotenv.2021.150328

    PMID: 34571217

    		 Sample: Liver Organoids (Los)
  115. CELLULAR & MOLECULAR BIOLOGY LETTERS (2023) IF: 8.702
    Formyl peptide receptor 2 activation by mitochondrial formyl peptides stimulates the neutrophil proinflammatory response via the ERK pathway and exacerbates ischemia–reperfusion injury

    DOI: 10.1186/s11658-023-00416-1
    Sample: Kidney Tissue
  116. ACS Applied Materials & Interfaces (2021) IF: 9.229
    Osmium–Tellurium Nanozymes for Pentamodal Combinatorial Cancer Therapy

    DOI: 10.1021/acsami.1c14201
    Sample: Ostenrs
  117. Journal of Advanced Research (2022) IF: 10.479
    Cadmium accumulation regulated by a rice heavy-metal importer is harmful for host plant and leaf bacteria

    DOI: 10.1016/j.jare.2022.05.010

    PMID: 35640876

    		 Sample: Leaf
  118. JOURNAL OF HAZARDOUS MATERIALS (2021) IF: 10.588
    Sequential use of UV-LEDs irradiation and chlorine to disinfect waterborne fungal spores: Efficiency, mechanism and photoreactivation

    DOI: 10.1016/j.jhazmat.2021.127102

    PMID: 34482083
  119. ACS Nano (2020) IF: 14.588
    Nonrecurring Circuit Nanozymatic Enhancement of Hypoxic Pancreatic Cancer Phototherapy Using Speckled Ru–Te Hollow Nanorods

    DOI: 10.1021/acsnano.9b09974
    Sample: Rutenrs
  120. Bioactive Materials (2023) IF: 16.874
    Gold nanoparticles-embedded ceria with enhanced antioxidant activities for treating inflammatory bowel disease

    DOI: 10.1016/j.bioactmat.2023.01.015
    Sample: Colon Tissue
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Reviews/Q&A

  • Show all
  • Reviews
  • Q&A

Verified Customer

Y*************rSubmitted [ Jun 23 2024 ]

  • Application:Biochemical
  • Description:Enzyme Stock Solution is not enough for 96 samples. To prepare Enzyme Working Solution 15 ul, it is recommended to use 0.5 ul Enzyme Stock Solution and 1 ul Enzyme Dilution Solution. So there is 1:2 ratio. When we use 300 ul Enzyme Stock solution then we need to add 600 Dilution solution. 900 ul of Enzyme working solution we will have. We need to put 20 ul each well. 900:20=45 It is enough for 45 samples only. How we will use it in 96 samples?
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Verified Customer

l**pSubmitted [ Jun 06 2019 ]

  • Application:Biochemical
  • Description:This kit has few sampling procedures, convenient operation, reasonable detection time, and the experimental results are clear and accurate, but the inhibition rate of this kit is difficult to grasp. When the dilution multiple of some samples reaches, the other part is on the high side or on the low side. If there are ways to improve or replace in this respect, the kit will be perfect.
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Verified Customer

s***rSubmitted [ May 31 2019 ]

  • Application:Biochemical
  • Description:The sample I tested was mouse serum,reference dilution given according to instructions, the kit need very little sample volume,besides, SOD kit easy to use and high sensitivity
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Verified Customer

M***ySubmitted [ May 31 2019 ]

  • Application:Biochemical
  • Description:The sample needs to be pre-tested, but the instructions provide dilutions range of the different sample. In general, the kit performs well
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Q

frSubmitted [ Jun 23 2024 ]

Asked: what is the dilution factor for chicken liver and breast meat samples?

Reply

A

adminSubmitted [ Jun 23 2024 ]

Answered: Our laboratory verified that the protein concentration of 10% chicken liver and 10% chicken tissue homogenization could be detected, the protein concentration in the supernatant was 11.764mg/mL and 3.714mg/mL, diluted 200 times and 60 times, respectively, and the T-SOD activity was 209.10U/mgprot and 126.65U/mgprot, respectively. You can refer to it, but there are differences between different samples, it is recommended that you choose 2-3 samples with large expected differences (different experimental groups) to do the pre-experiment first.

Q

C**wSubmitted [ Nov 09 2023 ]

Asked: May I know if the kit can be used on crude extracts such as ginger extract?

Reply

A

adminSubmitted [ Nov 09 2023 ]

Answered: Our lab has not verified the ginger extract sample before I regret. Do you want to detect plant samples? E-BC-K020-M can detect plant tissue samples, and our laboratory has verified 10% of green plants (760.426U/mgprot) and 20% of tomatoes (51.589U/mgprot). Theoretically, if the T-SDO enzyme activity in the extract is higher than the sensitivity of the kit, it can be detected. It is recommended that you buy a trial kit for pre-experiment first.

Q

m******eSubmitted [ Aug 30 2023 ]

Asked:  Do you have bacterial sample data? Any recommended usage amounts?

Reply

A

adminSubmitted [ Aug 30 2023 ]

Answered: The laboratory has not validated the bacterial sample, such samples are not available to the laboratory, and there is no mature assay protocol recommendations at present.

Q

N************RSubmitted [ Aug 05 2023 ]

Asked: Is this kit appropriate for studying with plants (lichens)?

Reply

A

adminSubmitted [ Aug 05 2023 ]

Answered: We'll reply to you by email. Pls pay attention to the mailbox

Q

D**********nSubmitted [ Apr 14 2023 ]

Asked: I want to know the SOD enzyme species

Reply

A

adminSubmitted [ Apr 14 2023 ]

Answered: There is no species distinction for metabolism assay.

Q

d*********aSubmitted [ Sep 26 2022 ]

Asked: Is the kit suitable for erythrocyte lysate?

Reply

A

adminSubmitted [ Sep 26 2022 ]

Answered: Dear Dragosalava, Thanks for your inquiry, we confirm with you that product #E-BC-K020-M, Total Superoxide Dismutase (T-SOD) Activity Assay Kit (WST-1 Method) is suitable for erythrocyte lysate.

Q

M*****aSubmitted [ Jul 28 2022 ]

Asked: Hello, I measured the SOD inhibition rate with liver tissue homogenate is about 80%, how many times should be diluted?

Reply

A

adminSubmitted [ Jul 28 2022 ]

Answered: It indicates that the dilution is still too low. It is recommended to increase the dilution about 200-300 times.

Q

M*****aSubmitted [ Jul 28 2022 ]

Asked: What is the dilution factor of the mouse serum?

Reply

A

adminSubmitted [ Jul 28 2022 ]

Answered: It is recommended to dilute for 20 times.

Q

N*rSubmitted [ Jul 19 2022 ]

Asked: I have problem with the calculation of inhibition ratio of SOD. What does Acontrol and Ablank control means? Where can I get the figures? Thank you

Reply

A

adminSubmitted [ Jul 19 2022 ]

Answered: If there are no samples added for the control hole and blank control. The corresponding OD value can be obtained after operation according to the instructions, which can be used for calculation.

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