Current Position:Home>>Kits>>ELISA Kits>>Uncoated ELISA Kits>> Uncoated Human IL-1β(Interleukin 1 Beta) ELISA Kit

Uncoated Human IL-1β(Interleukin 1 Beta) ELISA Kit

Uniprot : P01584

Cat.No.:E-UNEL-H0091
Reactivity: Human

Applications:ELISA

To Purchase E-UNEL-H0091

Size:

96T*5
96T*15

Price: $399

Qty:

Welcome to order from local distributors.

Protocols for ELISA

FAQ for ELISA

Have Questions?

Add to cart Bulk request

For research use only. Order now, ship in 3 days

Application

The new series of Elabscience^® Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.

For in vitro quantitative determination of IL-1β concentration in Human serum, plasma.

Properties

Assay type	Sandwich
Format	96T5 96T15
Assay time	24h
Reactivity	Human
Storage	-20℃.Please refer to the instructions for details.
Sample type	serum, plasma
Sample volume	100μL

Kit components

Item	Specifications	Storage
Micro ELISA pre-Plate(Dismountable)	96T*5：5 plates, 96T	-20℃, 12 months
Reference Standard	96T*5：1 vial
Concentrated Capture Ab	96T*5：1 vial 120μL
Concentrated Biotinylated Detection Ab	96T*5：1 vial 120μL
Concentrated HRP Conjugate	96T*5：1 vial 120μL	-20℃(Protect from light), 12 months
Product Description	1 copy	/
Certificate of Analysis	1 copy	/

Test Principle

	1. Analyte-specific antibody (capture antibody) is bound to a pre-microplate, unbound capture antibody is washed away. Then plates are blocked.
	2. Samples or standards are added and any analyte present is bound by the immobilized capture antibody. Unbound materials are decanted.
	3. The added Biotinylated Detection Ab will bind to the analyte been captured by the immobilized antibody. Unbound Biotinylated Detection Ab is washed away.
	4. HPR Conjugate is used to bind to the Biotinylated Detection Ab. Unbound HPR Conjugate is washed away.
	5. TMB substrate solution is added to the wells and a blue color develops in proportion to the amount of analyte present in the sample. Then the Stop Solution is added to stop the color change of substrate, and the color inside the well changes from blue to yellow. The absorbance of the color at 450 nm is measured.

Reviews/Q&A

Show all
Reviews
Q&A

s**************eSubmitted [ Jun 10 2024 ]

Asked: can I use this kit for samples from Caco-2 and HepG2 cell culture supernatants?

adminSubmitted [ Jun 10 2024 ]

Answered: Hi Solomon, We have validated E-UNEL-H0091 with unstimulated Hep G2 cell supernatant, samples were not diluted and the signal is very low. We have not verified Caco-2 cell supernatant. Since there are many factors affecting cells, it is recommended to do pre-experiments to confirm the sample concentration.

... Show All Show Less

*Product Name:
*Catalog Number:
Size:
*Name:
*Email:
*Phone:
*Message:
*Country:
*When will you use it?	Within 1 month In 2 to 3 month In 4 to 6 month 6 months later
*Captcha:	Click to load Captcha