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Uncoated Rat IL-6(Interleukin 6) ELISA Kit

Uniprot : P20607

Cat.No.:E-UNEL-R0030
Reactivity: Rat

Applications:ELISA

To Purchase E-UNEL-R0030

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96T*5
96T*15

Price: $399

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Application

The new series of Elabscience^® Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.

For in vitro quantitative determination of IL-6 concentration in Rat serum, plasma.

Properties

Assay type	Sandwich
Format	96T5 96T15
Assay time	24h
Reactivity	Rat
Storage	-20℃.Please refer to the instructions for details.
Sample type	serum, plasma
Sample volume	100μL

Kit components

Item	Specifications	Storage
Micro ELISA pre-Plate(Dismountable)	96T*5：5 plates, 96T	-20℃, 12 months
Reference Standard	96T*5：1 vial
Concentrated Capture Ab	96T*5：1 vial 120μL
Concentrated Biotinylated Detection Ab	96T*5：1 vial 120μL
Concentrated HRP Conjugate	96T*5：1 vial 120μL	-20℃(Protect from light), 12 months
Product Description	1 copy	/
Certificate of Analysis	1 copy	/

Test Principle

	1. Analyte-specific antibody (capture antibody) is bound to a pre-microplate, unbound capture antibody is washed away. Then plates are blocked.
	2. Samples or standards are added and any analyte present is bound by the immobilized capture antibody. Unbound materials are decanted.
	3. The added Biotinylated Detection Ab will bind to the analyte been captured by the immobilized antibody. Unbound Biotinylated Detection Ab is washed away.
	4. HPR Conjugate is used to bind to the Biotinylated Detection Ab. Unbound HPR Conjugate is washed away.
	5. TMB substrate solution is added to the wells and a blue color develops in proportion to the amount of analyte present in the sample. Then the Stop Solution is added to stop the color change of substrate, and the color inside the well changes from blue to yellow. The absorbance of the color at 450 nm is measured.

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