Click here to view cell apoptosis assay products
×

Uric Acid (UA) Colorimetric Assay Kit

  • Cat.No.:E-BC-K016-M

  • Detection instrument: Microplate reader(680-700 nm,optimum wavelength: 690 nm)

To Purchase E-BC-K016-M

Size:
  • 96T
  • 48T
Price: $180
Qty:

Welcome to order from local distributors.

Protocols for metabolism assays

FAQ for metabolism assays

Have Questions?

 Add to cart Bulk request

For research use only. Order now, ship in 3 days

Detection principle

Uric Acid in protein-free filtrate reduce phosphotungstic acid to form tungsten blue, allantoin and carbon dioxide, the depth of blue color is proportional to the concentration of uric acid. Uric acid content can be calculated by measuring the OD value at 690 nm.

Performance characteristics

Synonyms UA
Sample type Serum,plasma,urine
Sensitivity 1.30 mg/L
Detection range 1.30-80 mg/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 35 min
Precision Average inter-assay CV: 4%Average intra-assay CV: 2%
Other instruments required Micropipettor, Centrifuge, Vortex mixer
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (1.30-80 mg/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Mouse serum

1-2

Rat serum

1

Human serum

1

Porcine serum

1

Dog serum

1-2

Human urine

8-10

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Citations

  1. TOXICOLOGY AND INDUSTRIAL HEALTH (2019) IF: 1.635
    Toxic effects of subchronic oral acetamiprid exposure in rats:

    DOI: 10.1177/0748233719893203

    Sample: Plasma
  2. Drug Design Development and Therapy (2021) IF: 4.162
    Maackiain Protects the Kidneys of Type 2 Diabetic Rats via Modulating the Nrf2/HO-1 and TLR4/NF-κB/Caspase-3 Pathways

    DOI: 10.2147/DDDT.S326975

    PMID: 34703210

    Sample: Serum
  3. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE (2022) IF: 4.925
    Short-Term Hyperuricemia Leads to Structural Retinal Changes That Can be Reversed by Serum Uric Acid Lowering Agents in Mice

    DOI: 10.1167/iovs.63.10.8

    PMID: 36098977

    Sample: Serum
  4. Frontiers in Pharmacology (2021) IF: 5.811
    Pharmacological Basis for Use of a Novel Compound in Hyperuricemia: Anti-Hyperuricemic and Anti-Inflammatory Effects.

    DOI: 10.3389/fphar.2021.772504

    Sample: Serum
  5. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
    Ameliorative Effects of a Rhenium (V) Compound with Uracil-Derived Ligand Markers Associated with Hyperglycaemia-Induced Renal Dysfunction in Diet-Induced Prediabetic Rats

    DOI: 10.3390/ijms232315400

    Sample: Plasma,Urine
  6. Biomaterials Science (2022) IF: 7.59
    Transdermal delivery of allopurinol on acute hyperuricemic mice via polymer microneedles for regulation of serum uric acid levels

    DOI: 10.1039/D2BM01836E

    Sample: Serum
Show AllShorten

Reviews/Q&A

  • Show all
  • Reviews
  • Q&A
Q

C***kSubmitted [ Jun 23 2023 ]

Asked: Can I use this for saliva sample? Or are there anyways I can use this for saliva?

Reply

A

adminSubmitted [ Jun 23 2023 ]

Answered: Hi Clark, thanks for your support. You could use E-BC-K016-M for saliva sample.

Q

M**********dSubmitted [ Aug 25 2022 ]

Asked: How much sample do I need for an assay? I\'d like to take mouse serum.

Reply

A

adminSubmitted [ Aug 25 2022 ]

Answered: The assay takes as much as 25 micro-liter of serum sample, it's easy to get enough mouse serum.

Q

D********dSubmitted [ Aug 03 2022 ]

Asked: What operation is needed to prepare urine sample?

Reply

A

adminSubmitted [ Aug 03 2022 ]

Answered: Urine sample can be used directly after spinning to remove any suspended particular matter.

... Show All Show Less

People Also Bought

Apply for FREE TRIAL Fluorometric Assay kit
*Product Name:
*Catalog Number:
*Name:
*Email:
*Message:
*Country:
*When will you use it?

*Captcha: