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Uric Acid (UA) Colorimetric Assay Kit

Citations(6)

Cat.No.:E-BC-K016-M
Detection instrument: Microplate reader(680-700 nm,optimum wavelength: 690 nm)

Detection method: Colorimetric method

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Detection principle

Uric Acid in protein-free filtrate reduce phosphotungstic acid to form tungsten blue, allantoin and carbon dioxide, the depth of blue color is proportional to the concentration of uric acid. Uric acid content can be calculated by measuring the OD value at 690 nm.

Performance characteristics

Synonyms	UA
Sample type	Serum,plasma,urine
Sensitivity	1.30 mg/L
Detection range	1.30-80 mg/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	35 min
Precision	Average inter-assay CV: 4%Average intra-assay CV: 2%
Other instruments required	Micropipettor, Centrifuge, Vortex mixer
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (1.30-80 mg/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Mouse serum	1-2
Rat serum	1
Human serum	1
Porcine serum	1
Dog serum	1-2
Human urine	8-10

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

TOXICOLOGY AND INDUSTRIAL HEALTH (2019) IF: 1.635

Toxic effects of subchronic oral acetamiprid exposure in rats:

DOI: 10.1177/0748233719893203
	Sample: Plasma

Drug Design Development and Therapy (2021) IF: 4.162

Maackiain Protects the Kidneys of Type 2 Diabetic Rats via Modulating the Nrf2/HO-1 and TLR4/NF-κB/Caspase-3 Pathways

DOI: 10.2147/DDDT.S326975
PMID: 34703210	Sample: Serum

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE (2022) IF: 4.925

Short-Term Hyperuricemia Leads to Structural Retinal Changes That Can be Reversed by Serum Uric Acid Lowering Agents in Mice

DOI: 10.1167/iovs.63.10.8
PMID: 36098977	Sample: Serum

Frontiers in Pharmacology (2021) IF: 5.811

Pharmacological Basis for Use of a Novel Compound in Hyperuricemia: Anti-Hyperuricemic and Anti-Inflammatory Effects.

DOI: 10.3389/fphar.2021.772504
	Sample: Serum

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208

Ameliorative Effects of a Rhenium (V) Compound with Uracil-Derived Ligand Markers Associated with Hyperglycaemia-Induced Renal Dysfunction in Diet-Induced Prediabetic Rats

DOI: 10.3390/ijms232315400
	Sample: Plasma,Urine

Biomaterials Science (2022) IF: 7.59

Transdermal delivery of allopurinol on acute hyperuricemic mice via polymer microneedles for regulation of serum uric acid levels

DOI: 10.1039/D2BM01836E
	Sample: Serum

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Reviews/Q&A

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Reviews
Q&A

C***kSubmitted [ Jun 23 2023 ]

Asked: Can I use this for saliva sample? Or are there anyways I can use this for saliva?

adminSubmitted [ Jun 23 2023 ]

Answered: Hi Clark, thanks for your support. You could use E-BC-K016-M for saliva sample.

M**********dSubmitted [ Aug 25 2022 ]

Asked: How much sample do I need for an assay? I\'d like to take mouse serum.

adminSubmitted [ Aug 25 2022 ]

Answered: The assay takes as much as 25 micro-liter of serum sample, it's easy to get enough mouse serum.

D********dSubmitted [ Aug 03 2022 ]

Asked: What operation is needed to prepare urine sample?

adminSubmitted [ Aug 03 2022 ]

Answered: Urine sample can be used directly after spinning to remove any suspended particular matter.

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