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Uric Acid (UA) Fluorometric Assay Kit

Cat.No.:E-BC-F018
Detection instrument: Fluorescence microplate reader (Ex/Em=535 nm/587 nm)

Detection method: Fluorescence method

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Detection principle

Uricase catalyzes the decomposition of uric acid into allantoin, CO2 and H2O2. Under the action of peroxidase, H2O2 oxidizes the non-fluorescent probe into the fluorescent substance. By measuring the fluorescence value of the system, the corresponding uric acid content can be calculated.

Performance characteristics

Synonyms	UA
Sample type	Serum, plasma, urine, animal tissue
Sensitivity	0.03 μmol/L
Detection range	0.03-15 μmol/L
Detection method	Fluorescence method
Assay type	Quantitative
Assay time	40 min
Precision	Average inter-assay CV: 7.200%Average intra-assay CV: 1.500%
Other instruments required	Micropipettor, Vortex mixer, Centrifuge
Storage	-20℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.03-15 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	10-20
Human urine	80-100
Human hydrothorax	50-60
Rat urine	10-20
Rabbit serum	5-10
Rat serum	10-20
Porcine serum	1
10% Rat liver tissue homogenate	10-20
10% Rat kidney tissue homogenate	30-40
10% Rat lung tissue homogenate	10-20

Note: The diluent is reagent 1.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

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