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Vitamin C (VC) Colorimetric Assay Kit

Citations(6)

Cat.No.:E-BC-K034-M
Detection instrument: Microplate reader(530-540 nm,optimum wavelength: 536 nm)

Detection method: Colorimetric method

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Detection principle

The most obvious chemical activity of VC is that reduce Fe3+ to Fe2+, then promote iron absorption in the intestine, promote the storage and utilization of iron. Fe3+ react immediately with reducing ascorbic acid to form Fe2+. then Fe2+ react with phenanthroline and the color developing reaction occurs. The content of vitamin C in sample can be determined. Measure the OD value and calculate the VC content indirectly.

Performance characteristics

Synonyms	VC,Ascorbic acid
Sample type	Serum,plasma,tissue
Sensitivity	0.31 μg/mL
Detection range	0.31-17.5 μg/mL
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	50min
Precision	Average inter-assay CV: 6.100%Average intra-assay CV: 2.200%
Other instruments required	Micropipettor, Centrifuge, Incubator, Vortex mixer
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4), Absolute ethanol
Storage	2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.31-20 μg/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Mouse serum	1
Chicken serum	1
Horse serum	1
10% Mouse kidney tissue homogenate	1
10% Mouse liver tissue homogenate	1
10% Mouse lung tissue homogenate	1
10% Mouse spleen tissue homogenate	1
10% Rat liver tissue homogenate	1
10% Rat heart tissue homogenate	1
10% Plant tissue homogenate	1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Clinical Nutrition ESPEN (2021)

Vitamin C deficiency and risk of metabolic complications among adults with chronic respiratory diseases: A case–control study

DOI: 10.1016/j.clnesp.2021.03.007
	Sample: Serum

International Journal of General Medicine (2023) IF: 2.145

Hematological, Inflammatory, Coagulation, and Oxidative/Antioxidant Biomarkers as Predictors for Severity and Mortality in COVID-19: A Prospective Cohort-Study

DOI: 10.2147/IJGM.S402206
	Sample: Serum

LIFE SCIENCES (2020) IF: 3.647

Attenuation of chronic arsenic neurotoxicity via melatonin in male offspring of maternal rats exposed to arsenic during conception: Involvement of oxidative DNA damage and inflammatory signaling cascades

DOI: 10.1016/j.lfs.2020.118876
PMID: 33310035	Sample: Tissue

Scientific Reports (2019) IF: 4.011

Cold Atmospheric Plasma-Activated Water Irrigation Induces Defense Hormone and Gene expression in Tomato seedlings

DOI: 10.1038/s41598-019-52646-z
	Sample: Leaves,Roots

Frontiers in Psychiatry (2022) IF: 5.435

Efficacy of a novel proprietary dietary supplement (TRI 360TM) on psychological symptoms and stress-related quality of life in adult subjects: A randomized controlled clinical trial

DOI: 10.3389/fpsyt.2022.919284
PMID: 36032243	Sample: Serum

Nutrients (2023) IF: 6.706

Re-Visiting Antioxidant Therapy in Murine Advanced Atherosclerosis with Brussels Chicory, a Typical Vegetable in Mediterranean Diets

DOI: 10.3390/nu15040832
	Sample: Serum

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Reviews/Q&A

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Reviews
Q&A

c***aSubmitted [ Mar 13 2024 ]

Asked: sir plz tellme price in indianrupeees of vitamin E kit for research n blood

adminSubmitted [ Mar 13 2024 ]

Answered: Hello Sir,we will reply you by email.

DrSubmitted [ Jan 31 2023 ]

Asked: Is this kit useful for lens mater??

adminSubmitted [ Jan 31 2023 ]

Answered: This kit can be used to measure serum, plasma and tissue samples, and then it can be detected by enzyme marker and spectrophotometer.

M**uSubmitted [ Apr 21 2022 ]

Asked: Can this kit be used on cells?

adminSubmitted [ Apr 21 2022 ]

Answered: Dear Sir/Madam,thank for your message. E-BC-K034-M Vitamin C (VC) Colorimetric Assay Kit This product is not suitable for cell samples. best regards,

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