Introduction
Elabscience ® new generation of Western Blotting Antibody Dilution Buffer
is the highly effective primary and secondary antibody dilution buffer. It is
significantly better than the traditional primary and secondary antibody
diluent based on BSA, skimmed milk powder, etc., which can be used for the
dilution of Western blotting primary and secondary antibody.
Western
Blotting Antibody Dilution Buffer can effectively save antibody. The antibody
diluted and prepared by this product can be stored and used at 4°C for no less
than 6 months, and can be repeatedly used in Western Blotting, which is
beneficial to saving the antibodies.
This product contains optimized BSA, detergent and
antibody stabilizer, which can effectively reduce the combination of antibody
cost characteristics and improve the temperature preservation time of diluted
antibody.
This product has good compatibility. It is
compatible with horseradish peroxidase (HRP), alkaline phosphatase (AP) and
biotin labeled secondary antibody. This product does not contain biotin and
will not interfere with biotin based detection.
This product is easy to use, which does not need to
add any additional reagent, and can be directly used for the dilution of
antibodies.
Components
|
Cat
|
Products
|
100 mL
|
200 mL
|
500 mL
|
Storage
|
|
E-IR-R121
|
Western Blotting Antibody Dilution Buffer
|
100 mL
|
200 mL
|
500 mL
|
2~8°C
|
Experimental Procedure
1. Refer to the manuals of the primary or secondary antibody, and
the content of the target protein in the sample, dilute the primary or
secondary antibody according to the appropriate proportion, such as 1:50,
1:100, etc. (for the specific dilution ratio, please dilute according to the rate
of the manuals).
2. After the diluting of antibody with appropriate proportion, the
mixture can be directly used for the incubation of western blotting experiment.
Storage
Store at 2~8°C for 12 months. Avoid of freezing.
Cautions
1. This product couldn’t be used for clinical diagnosis or treatment, food or
medicine, and can’t be stored in residence.
2. For your safety and health,
please wear the lab coat and disposable gloves before the experiments.
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