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Xanthine Oxidase (XOD) Activity Fluorometric Assay Kit

Cat.No.:E-BC-F019
Detection instrument: Fluorescence microplate reader (Ex/Em=535 nm/587 nm)

Detection method: Fluorescence method

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96T

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Detection principle

Hypoxanthine are oxidized by xanthine oxidase (XOD) to produce xanthine and super oxygen anion, which will quickly converte to hydrogen peroxide in the system, and then, in the role of peroxidase, hydrogen peroxide can oxidize the non-fluorescent probe to fluorescent substance. By measuring the fluorescence value, the corresponding the activity of xanthine oxidase can be calculated.

Performance characteristics

Synonyms	XOD
Sample type	Serum, plasma, animal tissue
Sensitivity	0.01 U/L
Detection range	0.01 -1.2 U/L
Detection method	Fluorescence method
Assay type	Enzyme Activity
Assay time	25 min
Precision	Average inter-assay CV: 9%Average intra-assay CV: 4.100%
Other instruments required	Vortex mixer, Centrifuge
Storage	-20℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.01 -1.2 U/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Mouse serum	1
Dog serum	3-5
Rat plasma	1
10% Rat kidney tissue homogenate	5-10
Horse serum	1
Human plasma	1
10% Mouse heart tissue homogenate	5-10
10% Rat lung tissue homogenate	5-10

Note: The diluent is reagent 1.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

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