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Annexin V-Elab Fluor® 647/PI Apoptosis Kit

  • Cat.No.:E-CK-A213

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Detection principle

Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Annexin V-labeled fluorescein bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscope. Nuclear dyes (PI, DAPI, 7-AAD) have a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. Due to late apoptosis or loss of membrane integrity in necrotic cells, nuclear dyes can enter cells to stain DNA, and when used in combination with Annexin V, cells in different apoptotic stages can be distinguished.

General Information

Application Annexin V
Detection method Fluorometric Method
Sample type Cell samples
Assay time 40min
Detection instrument Flow Cytometer
Dye Type Elab Fluor® 647
Ex/Em (nm) 650/670
Channel set APC
Other reagents required PBS(E-BC-R187), Deionized water
Storage 2~8°C, shading light
Shipping Ice bag
Expiration date 12 months

Citations

  1. Purinergic Signalling (2022) IF: 3.765
    P2X7 receptor involved in antitumor activity of atractylenolide I in human cervical cancer cells

    DOI: 10.1007/s11302-022-09854-6

    PMID: 35235139

  2. Nanomaterials (2022) IF: 5.076
    Label-Free Morpho-Molecular Imaging for Studying the Differential Interaction of Black Phosphorus with Tumor Cells

    DOI: 10.3390/nano12121994

    PMID: 35745333

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