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Mitochondrial Membrane Potential Assay Kit (with JC-1)

  • Cat.No.:E-CK-A301

To Purchase E-CK-A301

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Detection principle

The decrease of mitochondrial membrane potential is a marker event in the early stage of apoptosis. It occurs before the appearance of nuclear apoptotic features (chromatin condensation and DNA fragmentation). Once the mitochondrial membrane potential collapses, apoptosis is irreversible. JC-1 is an ideal fluorescent probe widely used to detect mitochondrial membrane potential ∆Ψm. In normal cells, the mitochondrial membrane potential is high, JC-1 aggregates in the mitochondrial matrix to form a polymer, which can produce red fluorescence. In the early stage of apoptosis, the mitochondrial membrane potential decreases, JC-1 can’t accumulate in the mitochondrial matrix. When JC-1 is a monomer, it can produce green fluorescence. The decrease of cell membrane potential can be detected by the transition of JC-1 from red fluorescence to green fluorescence, and the transition of JC-1 fluorescence color can be used as an early detection indicator of cell apoptosis. The relative ratio of red and green fluorescence is commonly used to measure the ratio of mitochondrial depolarization. The maximum excitation wavelength of JC-1 monomer is 514 nm and the maximum emission wavelength is 529 nm; the maximum excitation wavelength of JC-1 polymer is 585 nm and the maximum emission wavelength is 590 nm.

General Information

Application JC-1
Detection method Fluorometric Method
Sample type Cell samples
Assay time 40min
Detection instrument Flow Cytometer, Fluorescence Microscope, Confocal Microscopes
Dye Type JC-1
Ex/Em (nm) 514/529, 585/590
Channel set FITC, PE
Filter set FITC, TRITC
Other reagents required Ultrapure water
Storage 2~8°C/-20°C, shading light. Refer to the Manual.
Shipping Ice bag
Expiration date 12 months

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. CNS Neuroscience & Therapeutics (2023) IF: 7.035
    Alpinetin inhibits neuroinflammation and neuronal apoptosis via targeting the JAK2/STAT3 signaling pathway in spinal cord injury

    DOI: 10.1111/cns.14085

  2. JOURNAL OF MEDICINAL CHEMISTRY (2022) IF: 7.446
    A Second Near-Infrared Ru(II) Polypyridyl Complex for Synergistic Chemo-Photothermal Therapy

    DOI: 10.1021/acs.jmedchem.1c01736

    PMID: 34994554

  3. Frontiers in Oncology (2021) IF: 6.244
    The Novel Curcumin Derivative 1g Induces Mitochondrial and ER-Stress-Dependent Apoptosis in Colon Cancer Cells by Induction of ROS Production

    DOI: 10.3389/fonc.2021.644197

  4. JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 4.36
    Norlignans and phenolics from genus Curculigo protect corticosterone-injured neuroblastoma cells SH-SY5Y by inhibiting endoplasmic reticulum stress-mitochondria pathway

    DOI: 10.1016/j.jep.2022.115430

    PMID: 35659626

  5. MEDICAL ONCOLOGY (2022) IF: 3.738
    Investigations on effects of titanium dioxide (TiO2) nanoparticle in combination with UV radiation on breast and skin cancer cells

    DOI: 10.1007/s12032-022-01931-5

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