Click here to view cell apoptosis assay products
×

Annexin V-Elab Fluor® Violet 500/PI Apoptosis Kit

  • Cat.No.:E-CK-A235

To Purchase E-CK-A235

Size:
  • 20Assays
  • 50Assays
  • 100Assays
  • 200Assays
Price: $75
Qty:

Detection principle

Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Annexin V-labeled fluorescein bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscope. Nuclear dyes (PI, DAPI, 7-AAD) have a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. Due to late apoptosis or loss of membrane integrity in necrotic cells, nuclear dyes can enter cells to stain DNA, and when used in combination with Annexin V, cells in different apoptotic stages can be distinguished.

General Information

Application Annexin V
Detection method Fluorometric Method
Sample type Cell samples
Assay time 40min
Detection instrument Flow Cytometer
Dye Type Elab Fluor® Violet 500
Ex/Em (nm) 425/500
Channel set Pacific Green
Other reagents required PBS(E-BC-R187), Deionized water
Storage 2~8°C, shading light
Shipping Ice bag
Expiration date 12 months

Reviews/Q&A

  • Show all
  • Reviews
  • Q&A
... Show All Show Less

People Also Bought

Apply for
*Product Name:
*Catalog Number:
*Name:
*Email:
*Message:
*Country:
*When will you use it?

*Captcha: