Alternate Names	Interferon gamma,IFNG,IFN-gamma,IFNγ
Swissprot	P01579
Gene ID	3458
References	1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321. 3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571. 4. Gray P, et al. 1987. Lymphokines 13:151.

Product Details

Form	Liquid
Clone No.	B27
Host	Mouse
Isotype	Mouse IgG1, κ
Isotype Control	APC Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09792E]
Reactivity	Human
Application	ICFCM
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	APC
	APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells. The B27 antibody reacts with the human interferon-γ. The B27 antibody can neutralize the bioactivity of natural or recombinant IFN-γ.

Protocols & Troubleshootings

Related Protocols	Staining Intracellular Antigens for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
AF/LE Purified Anti-Human IFN-γ Antibody[B27]	50μg, 500μg, 1mg	Check More
Purified Anti-Human IFN-γ Antibody[B27]	25μg, 100μg	Check More
FITC Anti-Human IFN-γ Antibody[B27]	20Tests, 100Tests, 100Tests×2	Check More
PE Anti-Human IFN-γ Antibody[B27]	20Tests, 100Tests, 100Tests×2	Check More

Citations

CYTOKINE (2022) IF: 3.926

Correlation between IFNAR1 expression in peripheral blood T lymphocytes and inflammatory cytokines, tumor-infiltrating lymphocytes, and chemosensitivity in patients with colorectal cancer

DOI: 10.1016/j.cyto.2022.156008
PMID: 36063748	Sample: Peripheral Blood T Cell

CELLULAR IMMUNOLOGY (2022) IF: 4.178

Human decidual gamma/delta T cells possess unique effector and TCR repertoire profiles during pregnancy

DOI: 10.1016/j.cellimm.2022.104634
PMID: 36308817	Sample: Pbmcs,Dmcs

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