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PE Anti-Human IFN-γ Antibody[B27]

Uniprot : P01579
  • Cat.No.:E-AB-F1196D

  • Applications: ICFCM
  • Isotype: Mouse IgG1, κ
  • Host: Mouse

To Purchase E-AB-F1196D

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  • 20Tests
  • 100Tests
  • 100Tests×2
Price: $75
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Antigen Details

Alternate Names Interferon gamma,IFNG,IFN-gamma,IFNγ
Swissprot P01579
Gene ID 3458
References
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.

Product Details

Form Liquid
Clone No. B27
Host Mouse
Isotype Mouse IgG1, κ
Isotype Control PE Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09792D]
Reactivity Human
Application ICFCM
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation PE
PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells. The B27 antibody reacts with the human interferon-γ. The B27 antibody can neutralize the bioactivity of natural or recombinant IFN-γ.

Protocols & Troubleshootings

Related Protocols Staining Intracellular Antigens for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Product Name Sizes Price
AF/LE Purified Anti-Human IFN-γ Antibody[B27] 50μg, 500μg, 1mg Check More
Purified Anti-Human IFN-γ Antibody[B27] 25μg, 100μg Check More
FITC Anti-Human IFN-γ Antibody[B27] 20Tests, 100Tests, 100Tests×2 Check More
APC Anti-Human IFN-γ Antibody[B27] 20Tests, 100Tests, 100Tests×2 Check More

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