Alternate Names	Integrin alpha-2,CD49 antigen-like family member B,Collagen receptor,Platelet membrane glycoprotein Ia,GPIa,VLA-2 subunit alpha,CD49b
Swissprot	Q62469
References	1. Arase H, et al. 2001. J. Immunol. 167:1141. 2. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 3. Sasaki K, et al. 2003. Int. Immunol. 15:701. 4. Inoue O, et al. 2003. J. Cell Biol. 160:769.

Product Details

Form	Liquid
Clone No.	DX5(See Other Available Formats)
Host	Rat
Isotype	Rat IgM, κ
Isotype Control	APC Rat IgM, κ Isotype Control[RTK2118] [Product E-AB-F09772E]
Reactivity	Mouse
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	APC
	APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

DX5 antigen has been recently characterized as CD49b. It is a 150 kD integrin α chain also known as α2 integrin, VLA-2 α chain, and integrin α2 chain. CD49b non-covalently associates with CD29 (β1 integrin) to form the CD49b/CD29 complex known as VLA-2, a receptor for collagen and laminin. CD49b is expressed on platelets, the majority of NK cells, NKT cells, and a small subset of CD8+ T cells (this population can be significantly increased following viral infection). DX5 is used for the identification and isolation of NK cells, and is especially useful for identifying NK cells in mice lacking the NK1.1 antigen.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
Biotin Anti-Mouse CD49b/pan-NK cells Antibody[DX5]	25μg, 100μg	Check More
FITC Anti-Mouse CD49b/pan-NK cells Antibody[DX5]	50Tests, 100Tests, 100Tests×2	Check More
PE Anti-Mouse CD49b/pan-NK cells Antibody[DX5]	50Tests, 100Tests, 100Tests×2	Check More
PerCP Anti-Mouse CD49b/pan-NK cells Antibody[DX5]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Mouse CD49b/pan-NK cells Antibody[DX5]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Mouse CD49b/pan-NK cells Antibody[DX5]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Mouse CD49b/pan-NK cells Antibody[DX5]	50Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Mouse CD49b/pan-NK cells Antibody[DX5]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Mouse CD49b/pan-NK cells Antibody[DX5]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Mouse CD49b/pan-NK cells Antibody[DX5]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Violet 450 Anti-Mouse CD49b/pan-NK cells Antibody[DX5]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Red 780 Anti-Mouse CD49b/pan-NK cells Antibody[DX5]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Mouse CD49b/pan-NK cells Antibody[DX5]	25μg, 100μg	Check More
PE Anti-Mouse CD49b/pan-NK cells Antibody[DX5]	25μg, 100μg	Check More
APC Anti-Mouse CD49b/pan-NK cells Antibody[DX5]	25μg, 100μg	Check More
PerCP Anti-Mouse CD49b/pan-NK cells Antibody[DX5]	25μg, 100μg	Check More
PE/Cyanine5 Anti-Mouse CD49b/pan-NK cells Antibody[DX5]	25μg, 100μg	Check More
PE/Cyanine7 Anti-Mouse CD49b/pan-NK cells Antibody[DX5]	25μg, 100μg	Check More
PE/Cyanine5.5 Anti-Mouse CD49b/pan-NK cells Antibody[DX5]	25μg, 100μg	Check More
PerCP/Cyanine5.5 Anti-Mouse CD49b/pan-NK cells Antibody[DX5]	25μg, 100μg	Check More
Elab Fluor^® 488 Anti-Mouse CD49b/pan-NK cells Antibody[DX5]	25μg, 100μg	Check More
Elab Fluor^® 647 Anti-Mouse CD49b/pan-NK cells Antibody[DX5]	25μg, 100μg	Check More
Elab Fluor^® Violet 450 Anti-Mouse CD49b/pan-NK cells Antibody[DX5]	25μg, 100μg	Check More
Elab Fluor^® Red 780 Anti-Mouse CD49b/pan-NK cells Antibody[DX5]	25μg, 100μg	Check More

Citations

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