Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> Biotin Anti-Mouse CD25 Antibody[PC-61.5.3]
Cat.No.:E-AB-F1102B
Welcome to order from local distributors.
FAQ for Flow Cytometry Antibody
Add to cart Bulk requestFor research use only. Order now, ship in 3 days
Alternate Names | Interleukin-2 receptor subunit alpha,IL2RA,IL-2 receptor subunit alpha,IL-2-RA,IL-2R subunit alpha,IL2-RA,TAC antigen,p55,p55 |
Swissprot | P01590 |
References |
1. Taniguchi T, et al. 1993. Cell 73:5.
2. Waldmann TA. 1991. J. Biol. Chem. 266:2681. 3. Read S, et al. 2000. J. Exp. Med. 192:295. 4. Lowenthal JW, et al. 1985. J. Immunol. 135:3988. |
Form | Liquid |
Clone No. | PC-61.5.3 |
Host | Rat |
Isotype | Rat IgG1, κ |
Isotype Control | Biotin Rat IgG1, κ Isotype Control[HRPN] [Product E-AB-F09823B] |
Reactivity | Mouse |
Application | FCM |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | Biotin |
How to select the appropriate detection channel through the spectrogram?
CD25 is a 55 kD glycoprotein, also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subset, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132(common γ chain), CD25 forms the high affinity signaling IL-2 receptor. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |