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PE Anti-Mouse CD25 Antibody[PC-61.5.3]

Uniprot : P01590
  • Cat.No.:E-AB-F1102D

  • Applications: FCM
  • Isotype: Rat IgG1, κ
  • Host: Rat

To Purchase E-AB-F1102D

Size:
  • 50Tests
  • 100Tests
  • 100Tests×2
Price: $65
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Antigen Details

Alternate Names Interleukin-2 receptor subunit alpha,IL2RA,IL-2 receptor subunit alpha,IL-2-RA,IL-2R subunit alpha,IL2-RA,TAC antigen,p55,p55
Swissprot P01590
References
1. Taniguchi T, et al. 1993. Cell 73:5.
2. Waldmann TA. 1991. J. Biol. Chem. 266:2681.
3. Read S, et al. 2000. J. Exp. Med. 192:295.
4. Lowenthal JW, et al. 1985. J. Immunol. 135:3988.

Product Details

Form Liquid
Clone No. PC-61.5.3(See Other Available Formats)
Host Rat
Isotype Rat IgG1, κ
Isotype Control PE Rat IgG1, κ Isotype Control[HRPN] [Product E-AB-F09822D]
Reactivity Mouse
Application FCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation PE
PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

CD25 is a 55 kD glycoprotein, also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subset, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132(common γ chain), CD25 forms the high affinity signaling IL-2 receptor.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Product Name Sizes Price
AF/LE Purified Anti-Mouse CD25 Antibody[PC-61.5.3] 50μg, 500μg, 1mg Check More
Purified Anti-Mouse CD25 Antibody[PC-61.5.3] 25μg, 100μg Check More
Biotin Anti-Mouse CD25 Antibody[PC-61.5.3] 25μg, 100μg Check More
FITC Anti-Mouse CD25 Antibody[PC-61.5.3] 50Tests, 100Tests, 100Tests×2 Check More
APC Anti-Mouse CD25 Antibody[PC-61.5.3] 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5 Anti-Mouse CD25 Antibody[PC-61.5.3] 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine7 Anti-Mouse CD25 Antibody[PC-61.5.3] 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5.5 Anti-Mouse CD25 Antibody[PC-61.5.3] 50Tests, 100Tests, 100Tests×2 Check More
PerCP/Cyanine5.5 Anti-Mouse CD25 Antibody[PC-61.5.3] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 488 Anti-Mouse CD25 Antibody[PC-61.5.3] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 647 Anti-Mouse CD25 Antibody[PC-61.5.3] 50Tests, 100Tests, 100Tests×2 Check More
PE/Elab Fluor® 594 Anti-Mouse CD25 Antibody[PC-61.5.3] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® Violet 450 Anti-Mouse CD25 Antibody[PC-61.5.3] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® Red 780 Anti-Mouse CD25 Antibody[PC-61.5.3] 50Tests, 100Tests, 100Tests×2 Check More
FITC Anti-Mouse CD25 Antibody[PC-61.5.3] 25μg, 100μg Check More
PE Anti-Mouse CD25 Antibody[PC-61.5.3] 25μg, 100μg Check More
APC Anti-Mouse CD25 Antibody[PC-61.5.3] 25μg, 100μg Check More
PE/Cyanine5 Anti-Mouse CD25 Antibody[PC-61.5.3] 25μg, 100μg Check More
PE/Cyanine7 Anti-Mouse CD25 Antibody[PC-61.5.3] 25μg, 100μg Check More
PE/Cyanine5.5 Anti-Mouse CD25 Antibody[PC-61.5.3] 25μg, 100μg Check More
PerCP/Cyanine5.5 Anti-Mouse CD25 Antibody[PC-61.5.3] 25μg, 100μg Check More
Elab Fluor® 488 Anti-Mouse CD25 Antibody[PC-61.5.3] 25μg, 100μg Check More
Elab Fluor® 647 Anti-Mouse CD25 Antibody[PC-61.5.3] 25μg, 100μg Check More
PE/Elab Fluor® 594 Anti-Mouse CD25 Antibody[PC-61.5.3] 25μg, 100μg Check More
Elab Fluor® Violet 450 Anti-Mouse CD25 Antibody[PC-61.5.3] 25μg, 100μg Check More
Elab Fluor® Red 780 Anti-Mouse CD25 Antibody[PC-61.5.3] 25μg, 100μg Check More

Citations

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