Alternate Names	Interleukin-2 receptor subunit alpha,IL2RA,IL-2 receptor subunit alpha,IL-2-RA,IL-2R subunit alpha,IL2-RA,TAC antigen,p55,p55
Swissprot	P01590
References	1. Taniguchi T, et al. 1993. Cell 73:5. 2. Waldmann TA. 1991. J. Biol. Chem. 266:2681. 3. Read S, et al. 2000. J. Exp. Med. 192:295. 4. Lowenthal JW, et al. 1985. J. Immunol. 135:3988.

Product Details

Form	Liquid
Clone No.	PC-61.5.3
Host	Rat
Isotype	Rat IgG1, κ
Isotype Control	PE/Cyanine7 Rat IgG1, κ Isotype Control[HRPN] [Product E-AB-F09823H]
Reactivity	Mouse
Application	FCM
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/10⁶ cells in 100 μL volume].
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	PE/Cyanine7
	PE/Cyanine7 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 775 nm (e.g., a 780/60 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD25 is a 55 kD glycoprotein, also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subset, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132(common γ chain), CD25 forms the high affinity signaling IL-2 receptor.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
AF/LE Purified Anti-Mouse CD25 Antibody[PC-61.5.3]	50μg, 500μg, 1mg	Check More
Purified Anti-Mouse CD25 Antibody[PC-61.5.3]	25μg, 100μg	Check More
Biotin Anti-Mouse CD25 Antibody[PC-61.5.3]	25μg, 100μg	Check More
FITC Anti-Mouse CD25 Antibody[PC-61.5.3]	50Tests, 100Tests, 100Tests×2	Check More
PE Anti-Mouse CD25 Antibody[PC-61.5.3]	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Mouse CD25 Antibody[PC-61.5.3]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Mouse CD25 Antibody[PC-61.5.3]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Mouse CD25 Antibody[PC-61.5.3]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Mouse CD25 Antibody[PC-61.5.3]	50Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Mouse CD25 Antibody[PC-61.5.3]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Mouse CD25 Antibody[PC-61.5.3]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Mouse CD25 Antibody[PC-61.5.3]	50Tests, 100Tests, 100Tests×2	Check More
PE/Elab Fluor^® 594 Anti-Mouse CD25 Antibody[PC-61.5.3]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Violet 450 Anti-Mouse CD25 Antibody[PC-61.5.3]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Red 780 Anti-Mouse CD25 Antibody[PC-61.5.3]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Mouse CD25 Antibody[PC-61.5.3]	25μg, 100μg	Check More
PE Anti-Mouse CD25 Antibody[PC-61.5.3]	25μg, 100μg	Check More
APC Anti-Mouse CD25 Antibody[PC-61.5.3]	25μg, 100μg	Check More
PE/Cyanine5 Anti-Mouse CD25 Antibody[PC-61.5.3]	25μg, 100μg	Check More
PE/Cyanine5.5 Anti-Mouse CD25 Antibody[PC-61.5.3]	25μg, 100μg	Check More
PerCP/Cyanine5.5 Anti-Mouse CD25 Antibody[PC-61.5.3]	25μg, 100μg	Check More
Elab Fluor^® 488 Anti-Mouse CD25 Antibody[PC-61.5.3]	25μg, 100μg	Check More
Elab Fluor^® 647 Anti-Mouse CD25 Antibody[PC-61.5.3]	25μg, 100μg	Check More
PE/Elab Fluor^® 594 Anti-Mouse CD25 Antibody[PC-61.5.3]	25μg, 100μg	Check More
Elab Fluor^® Violet 450 Anti-Mouse CD25 Antibody[PC-61.5.3]	25μg, 100μg	Check More
Elab Fluor^® Red 780 Anti-Mouse CD25 Antibody[PC-61.5.3]	25μg, 100μg	Check More

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