Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> Elab Fluor® Violet 450 Anti-Mouse CD16/32 Antibody[2.4G2]
Cat.No.:E-AB-F0997UQ
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Alternate Names | CD16a/b,CD32,CD32A/B,Fc fragment of IgG low affinity IIa/b receptor,Fc fragment of IgG low affinity IIIa/b receptor,Fc fragment of IgG low affinity IIIb receptor,Fc gamma receptor III A/B,FCG2A,FcGR,FCGR2A/BFCGR3,FCGR3A/B,Fc gamma RIIa/b |
Swissprot | P08508,P08101 |
Gene ID | 14130,14131 |
References |
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Unkeless JC. 1989. J. Clin. Invest. 83:355. 3. Qiu WQ, et al. 1990. Science 248:732. |
Form | Liquid |
Clone No. | 2.4G2 |
Host | Rat |
Isotype | Rat IgG2b, κ |
Isotype Control | Elab Fluor® Violet 450 Rat IgG2b, κ Isotype Control[LTF-2] [Product E-AB-F09843Q] |
Reactivity | Mouse |
Application | FCM |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume]. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | Elab Fluor® Violet 450 |
Elab Fluor® Violet 450 is designed to be excited by the violet laser (405 nm) and detected using an optical filter centered near 450 nm (e.g., a 450/45 nm bandpass filter). | |
How to select the appropriate detection channel through the spectrogram?
CD16 is low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |