Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> PerCP/Cyanine5.5 Anti-Mouse CD16/32 Antibody[2.4G2]
Cat.No.:E-AB-F0997J
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Alternate Names | CD16a/b,CD32,CD32A/B,Fc fragment of IgG low affinity IIa/b receptor,Fc fragment of IgG low affinity IIIa/b receptor,Fc fragment of IgG low affinity IIIb receptor,Fc gamma receptor III A/B,FCG2A,FcGR,FCGR2A/BFCGR3,FCGR3A/B,Fc gamma RIIa/b |
Swissprot | P08508,P08101 |
References |
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Unkeless JC. 1989. J. Clin. Invest. 83:355. 3. Qiu WQ, et al. 1990. Science 248:732. |
Form | Liquid |
Clone No. | 2.4G2 |
Host | Rat |
Isotype | Rat IgG2b, κ |
Isotype Control | PerCP/Cyanine5.5 Rat IgG2b, κ Isotype Control[LTF-2] [Product E-AB-F09842J] |
Reactivity | Mouse |
Application | FCM |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | PerCP/Cyanine5.5 |
PerCP/Cyanine5.5 is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 675 nm (e.g., a 690/50 nm bandpass filter). | |
How to select the appropriate detection channel through the spectrogram?
CD16 is low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |