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FITC Anti-Human CD4 Antibody[RPA-T4]

Name: FITC Anti-Human CD4 Antibody[RPA-T4]
Brand: Elabscience
SKU: E-AB-F1109C
Price: 13 USD
Availability: InStock
Rating: 5 (1 reviews)

Uniprot : P01730

Cat.No.:E-AB-F1109C
Applications: FCM
Isotype: Mouse IgG1, κ
Host: Mouse

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Antigen Details

Alternate Names	T-cell surface glycoprotein CD4,CD4,T-cell surface antigen T4/Leu-3,CD4
Swissprot	P01730
References	1. Zhu J, et al. 2010. Annu Rev. Immunol. 28:445. 2. Vignali DA. 2010. J. Immunol. 184:5933. 3. Zhou L, et al. 2009. Immunity 30:646. 4. Singer A, et al. 2008. Nat. Rev. Immunol. 8:788. 5. Zhu J and Paul WE. 2008. Blood 112:1557.

Product Details

Form	Liquid
Clone No.	RPA-T4
Host	Mouse
Isotype	Mouse IgG1, κ
Isotype Control	FITC Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09792C]
Reactivity	Human
Application	FCM
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	FITC
	FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD4, also known as T4/Leu-3, is a 55 kD single-chain type I transmembrane glycoprotein and member of the immunoglobulin superfamily. It is expressed on most thymocytes, helper T cells, type II NKT cells, and monocytes/macrophages. CD4 is part of the TCR/CD3 complex, binds to β2 domain from the MHC class II molecule, and participates in TCR signal transduction. CD4 is the receptor of IL-16 and is a coreceptor for the human immunodeficiency virus (HIV) and human herpes virus 7 (HHV-7).

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

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Reviews/Q&A

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Verified Customer

f***kSubmitted [ Jun 10 2019 ]

Application:FCM
Species:Human
Sample:blood
Primary antibody:Undiluted
30minutes

Temperature:4℃

Description:this cd4 Monoclonal Antibody is used well and i can see two cell populations

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