Alternate Names	T-cell surface glycoprotein CD4,CD4,T-cell surface antigen T4/Leu-3,CD4
Swissprot	P01730
References	1. Zhu J, et al. 2010. Annu Rev. Immunol. 28:445. 2. Vignali DA. 2010. J. Immunol. 184:5933. 3. Zhou L, et al. 2009. Immunity 30:646. 4. Singer A, et al. 2008. Nat. Rev. Immunol. 8:788. 5. Zhu J and Paul WE. 2008. Blood 112:1557.

Product Details

Form	Liquid
Clone No.	RPA-T4
Host	Mouse
Isotype	Mouse IgG1, κ
Isotype Control	PE/Cyanine7 Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09792H]
Reactivity	Human
Application	FCM
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	PE/Cyanine7
	PE/Cyanine7 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 775 nm (e.g., a 780/60 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD4, also known as T4/Leu-3, is a 55 kD single-chain type I transmembrane glycoprotein and member of the immunoglobulin superfamily. It is expressed on most thymocytes, helper T cells, type II NKT cells, and monocytes/macrophages. CD4 is part of the TCR/CD3 complex, binds to β2 domain from the MHC class II molecule, and participates in TCR signal transduction. CD4 is the receptor of IL-16 and is a coreceptor for the human immunodeficiency virus (HIV) and human herpes virus 7 (HHV-7).

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
AF/LE Purified Anti-Human CD4 Antibody[RPA-T4]	50μg, 500μg, 1mg	Check More
Purified Anti-Human CD4 Antibody[RPA-T4]	25μg, 100μg	Check More
Biotin Anti-Human CD4 Antibody[RPA-T4]	25μg, 100μg	Check More
FITC Anti-Human CD4 Antibody[RPA-T4]	20Tests, 100Tests, 100Tests×2	Check More
PE Anti-Human CD4 Antibody[RPA-T4]	20Tests, 100Tests, 100Tests×2	Check More
APC Anti-Human CD4 Antibody[RPA-T4]	20Tests, 100Tests, 100Tests×2	Check More
PerCP Anti-Human CD4 Antibody[RPA-T4]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Human CD4 Antibody[RPA-T4]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Human CD4 Antibody[RPA-T4]	20Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Human CD4 Antibody[RPA-T4]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Human CD4 Antibody[RPA-T4]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Human CD4 Antibody[RPA-T4]	20Tests, 100Tests, 100Tests×2	Check More
PE/Elab Fluor^® 594 Anti-Human CD4 Antibody[RPA-T4]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Violet 450 Anti-Human CD4 Antibody[RPA-T4]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Red 780 Anti-Human CD4 Antibody[RPA-T4]	20Tests, 100Tests, 100Tests×2	Check More

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