Free fatty acids (NEFA/FFA) generate acyl-CoA under the action of acyl synthase, then acyl-CoA generates hydrogen peroxide under the oxidation of acyl oxidase. Hydrogen peroxide reacts in the presence of enzymes and chromogens, producing a red substance with maximum absorption at 546 nm. The amount of this red product is proportional to the content of free fatty acids.

Performance characteristics

Sample type	serum;plasma;animal tissue;cell
Sensitivity	0.14 mmol/L
Detection range	0.14-4 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	30 min
Precision	Average inter-assay CV: 2.5-6.1%Average intra-assay CV: 1.5-4.5%
Other reagents required	Normal saline (0.9% NaCl), Double distilled water
Storage	Reagent2 -20℃, others 2-8℃
Valid period	12 months

Ralated product

Cat Number	Product Name
E-BC-K091-M	Alkaline Phosphatase (ALP) Activity Assay Kit
E-BC-F033	Triglyceride (TG) Fluorometric Assay Kit
E-BC-K109-M	Total Cholesterol (TC) Colorimetric Assay Kit (Single Reagent, COD-PAP Method)

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

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Cat. No	E-BC-K792-M
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