Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> PE/Cyanine5 Anti-Human CD127/IL-7RA Antibody[A019D5]

PE/Cyanine5 Anti-Human CD127/IL-7RA Antibody[A019D5]

Uniprot : P16871

Cat.No.:E-AB-F1152G
Applications: FCM
Isotype: Mouse IgG1, κ
Host: Mouse

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Antigen Details

Alternate Names	Interleukin-7 receptor subunit alpha,IL-7RA,CDw127,CD127,IL-7Rα
Swissprot	P16871
References	1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125. 2. He YW and Malek TR. 1998. Crit. Rev. Immunol. 18:503. 3. Huster KM, et al. 2004. P. Natl. Acad. Sci. USA 101:5610. 4. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403. 5. Morrissey PJ, et al. 1989. J. Exp. Med. 169:707. 6. Liu W, et al. 2006. J. Exp. Med. 203:1701.

Product Details

Form	Liquid
Clone No.	A019D5
Host	Mouse
Isotype	Mouse IgG1, κ
Isotype Control	[Product E-AB-F09792G]
Reactivity	Human
Application	FCM
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	PE/Cyanine5
	PE/Cyanine5 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 670 nm (e.g., a 690/50 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage cells, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be a useful marker for identifying memory and effector T cells. Studies have shown that CD127 expression is down-modulated on Treg cells. It can be used as a marker for differentiation of Treg and conventional T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cell proliferation and development.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
Biotin Anti-Human CD127/IL-7RA Antibody[A019D5]	25μg, 100μg	Check More
FITC Anti-Human CD127/IL-7RA Antibody[A019D5]	20Tests, 100Tests, 100Tests×2	Check More
PE Anti-Human CD127/IL-7RA Antibody[A019D5]	20Tests, 100Tests, 100Tests×2	Check More
APC Anti-Human CD127/IL-7RA Antibody[A019D5]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Human CD127/IL-7RA Antibody[A019D5]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Human CD127/IL-7RA Antibody[A019D5]	20Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Human CD127/IL-7RA Antibody[A019D5]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Human CD127/IL-7RA Antibody[A019D5]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Human CD127/IL-7RA Antibody[A019D5]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Violet 450 Anti-Human CD127/IL-7RA Antibody[A019D5]	20Tests, 100Tests, 100Tests×2	Check More

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