In the presence of adenosine diphosphate (ADP), pyruvate kinase (PK) catalyzes the conversion of phosphoenolpyruvate (PEP) to pyruvate, Lactate dehydrogenase catalyzes the reaction of pyruvic acid with NADH to produce lactic acid and NAD+. The activity of pyruvate kinase can be calculated by detection the absorbance changes at 340 nm.

Performance characteristics

Synonyms	PK
Sample type	serum, plasma, tissue and cell
Sensitivity	0.08 U/L
Detection range	0.08–6.79 U/L
Assay type	Enzyme Activity
Assay time	10 min
Precision	Average inter-assay CV: 7%Average intra-assay CV: 5%
Other instruments required	Test tube, Micropipettor, Vortex mixer, Incubator
Storage	-20℃
Valid period	12 months

Ralated product

Cat Number	Product Name
E-BC-K130-M	Pyruvic Acid Colorimetric Assay Kit

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

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Cat. No	E-BC-K611-M
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Pyruvate Kinase (PK) Activity Assay Kit

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Detection principle

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Pyruvate Kinase (PK) Activity Assay Kit

To Purchase E-BC-K611-M

Detection principle

Performance characteristics

Ralated product

Troubleshooting

Reviews/Q&A

People Also Bought

About Us

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United States

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Apply for FREE TRIAL Fluorometric Assay kit

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