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Annexin V-FITC/7-AAD Apoptosis Kit

  • Cat.No.:E-CK-A212

To Purchase E-CK-A212

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Detection principle

Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Annexin V-labeled fluorescein bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscope. Nuclear dyes (PI, DAPI, 7-AAD) have a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. Due to late apoptosis or loss of membrane integrity in necrotic cells, nuclear dyes can enter cells to stain DNA, and when used in combination with Annexin V, cells in different apoptotic stages can be distinguished.

General Information

Application Annexin V
Detection method Fluorometric Method
Sample type Cell samples
Assay time 40min
Detection instrument Flow Cytometer
Dye Type FITC
Ex/Em (nm) 490/530
Channel set FITC
Other reagents required PBS(E-BC-R187), Deionized water
Storage 2~8°C, shading light.
Shipping Ice bag
Expiration date 12 months

Citations

  1. JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 5.195
    Mahuang Xixin Fuzi decoction ameliorates apoptosis via the mitochondrial-mediated signaling pathway in MCM cells

    DOI: 10.1016/j.jep.2022.115538

    PMID: 35843410

  2. Experimental and Therapeutic Medicine (2019) IF: 1.448
    Mechanism of IL‑8‑induced acute lung injury through pulmonary surfactant proteins A and B

    DOI: 10.3892/etm.2019.8192

    Sample: Cell Sample
  3. Experimental and Therapeutic Medicine (2019) IF: 1.448
    Downregulation of miR‑552 in hepatocellular carcinoma inhibits cell migration and invasion, and promotes cell apoptosis via RUNX3

    DOI: 10.3892/etm.2019.8061

    Sample: Cell Sample
  4. INTERNATIONAL IMMUNOPHARMACOLOGY (2020) IF: 3.943
    Antileishmanial activity of cordiaquinone E towards Leishmania (Leishmania) amazonensis

    DOI: 10.1016/j.intimp.2020.107124

    PMID: 33168414

    Sample: Cell Sample
  5. CELLULAR SIGNALLING (2022) IF: 4.85
    miR-24-3p down-regulates the expression of the apoptotic factors FasL and BIM in human natural killer cells

    DOI: 10.1016/j.cellsig.2022.110415

    PMID: 35870695

  6. INTERNATIONAL IMMUNOPHARMACOLOGY (2021) IF: 4.932
    Curzerene antileishmania activity: Effects on Leishmania amazonensis and possible action mechanisms

    DOI: 10.1016/j.intimp.2021.108130

    PMID: 34500286

  7. Cancer Cell International (2022) IF: 5.722
    A synthesized olean-28,13β-lactam targets YTHDF1-GLS1 axis to induce ROS-dependent metabolic crisis and cell death in pancreatic adenocarcinoma

    DOI: 10.1186/s12935-022-02562-6

    PMID: 35366902

  8. Frontiers in Oncology (2021) IF: 6.244
    Discovering and Characterizing of Survivin Dominant Negative Mutants With Stronger Pro-apoptotic Activity on Cancer Cells and CSCs

    DOI: 10.3389/fonc.2021.635233

  9. Acta Biomaterialia (2022) IF: 8.947
    Preparation of epigallocatechin gallate decorated Au-Ag nano-heterostructures as NIR-sensitive nano-enzymes for the treatment of osteoarthritis through mitochondrial repair and cartilage protection

    DOI: 10.1016/j.actbio.2022.03.038

    PMID: 35358735

  10. ACS Applied Materials & Interfaces (2021) IF: 9.229
    Fully Integrated Self-Powered Electrical Stimulation Cell Culture Dish for Noncontact High-Efficiency Plasmid Transfection

    DOI: 10.1021/acsami.1c16748

    PMID: 34757708

    Sample: A549 Cells,Hepg2 Cells
  11. ACS Applied Materials & Interfaces (2022) IF: 10.383
    Ultrasound-Mediated Antitumor Therapy via Targeted Acoustic Release Carrier of Carbon Monoxide (TARC-CO)

    DOI: 10.1021/acsami.2c16821

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Verified Customer

R**USubmitted [ Dec 04 2019 ]

  • Application:FCM
  • Description:I used it well,it is good .I can get the desired results very well. I can see apoptotic cells well
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