Current Position:Home>>Cell Products>>Cell Apoptosis Assay>>Annexin V>>Annexin V Apoptosis Detection Kits>> Annexin V-FITC/PI Apoptosis Kit
Cat.No.:E-CK-A211
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Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Annexin V-labeled fluorescein bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscope. Nuclear dyes (PI, DAPI, 7-AAD) have a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. Due to late apoptosis or loss of membrane integrity in necrotic cells, nuclear dyes can enter cells to stain DNA, and when used in combination with Annexin V, cells in different apoptotic stages can be distinguished.
Application | Annexin V |
Detection method | Fluorometric Method |
Sample type | Cell samples |
Assay time | 40min |
Detection instrument | Flow Cytometer |
Dye Type | FITC |
Ex/Em (nm) | 490/530 |
Channel set | FITC |
Other reagents required | PBS(E-BC-R187), Deionized water |
Storage | 2~8°C, shading light |
Shipping | Ice bag |
Expiration date | 12 months |
PMID: 35150848 |
PMID: 34634461 |
DOI: 10.3390/ijms24043561 |
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DOI: 10.1111/jog.15003 |
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PMID: 34482598 |
PMID: 35689754 |
DOI: 10.1093/bbb/zbaa106 |
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DOI: 10.1155/2022/4534411 |
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PMID: 35615689 |
PMID: 34102026 |
DOI: 10.1159/000514209 |
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PMID: 34428767 |
DOI: 10.2147/CMAR.S280124 |
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PMID: 33204164 |
Sample: Cell Sample |
PMID: 34235609 |
Sample: Cell Lysate |
PMID: 34427908 |
PMID: 34560189 |
PMID: 34052562 |
Sample: Cell Sample |
PMID: 34895046 |
PMID: 35311620 |
PMID: 35510365 |
PMID: 36095918 |
PMID: 34255431 |
PMID: 33681580 |
Sample: Cell(Myocardial) |
PMID: 35367832 |
PMID: 34461437 |
PMID: 35751788 |
PMID: 36161101 |
PMID: 35123552 |
PMID: 35642312 |
PMID: 35916967 |
PMID: 33765653 |
Sample: Cell Sample |
PMID: 35523306 |
DOI: 10.3892/or.2021.8241 |
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PMID: 34913075 |
DOI: 10.3390/biom10020217 |
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DOI: 10.1002/tox.23540 |
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PMID: 35442555 |
DOI: 10.1002/tox.23341 |
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PMID: 34351699 |
PMID: 32316661 |
Sample: Cell Sample |
PMID: 35504558 |
DOI: 10.7150/jca.62630 |
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PMID: 34976175 |
PMID: 33743811 |
Sample: Cell Sample |
DOI: 10.1002/ddr.21835 |
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PMID: 34056735 |
Sample: Cell Sample |
PMID: 35304586 |
DOI: 10.1155/2022/7888937 |
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PMID: 36213491 |
PMID: 35744886 |
DOI: 10.1111/jre.12973 |
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PMID: 35037251 |
DOI: 10.1093/jpp/rgac072 |
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PMID: 36264186 |
PMID: 32710727 |
Sample: Cell Sample |
DOI: 10.7150/ijbs.41293 |
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PMID: 36080236 |
PMID: 33359068 |
PMID: 34081990 |
Sample: Tissue Homogenate |
PMID: 35714705 |
DOI: 10.3390/ph16020205 |
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DOI: 10.3390/ph15091075 |
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PMID: 34801948 |
PMID: 35679795 |
DOI: 10.2139/ssrn.3951497 |
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PMID: 35788718 |
PMID: 36279666 |
DOI: 10.3390/nu14091835 |
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PMID: 35565803 |
PMID: 34020639 |
Sample: Cell Sample |
DOI: 10.3390/ijms22115649 |
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PMID: 34073318 |
Sample: Cell Sample |
DOI: 10.3390/ijms24020919 |
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PMID: 35691198 |
PMID: 35214139 |
PMID: 34575494 |
DOI: 10.21037/tlcr-21-145 |
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PMID: 33718039 |
Sample: Cell Sample(A549/Taxr Cells,A549 Cells) |
PMID: 35551039 |
PMID: 35617856 |
PMID: 35954399 |
DOI: 10.1155/2022/5888636 |
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PMID: 36076588 |
PMID: 36017794 |
PMID: 36271582 |
PMID: 36306591 |
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PMID: 35772376 |
PMID: 35805188 |
PMID: 36231056 |
PMID: 35752246 |
PMID: 35217832 |
PMID: 36167821 |
PMID: 36202150 |
PMID: 35907592 |
DOI: 10.1111/jpi.12819 |
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PMID: 35906194 |
PMID: 35921728 |
PMID: 35864549 |
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w**tSubmitted [ Dec 04 2019 ]
g*********kSubmitted [ Jun 06 2024 ]
Asked: I asked a question: The protocol states \\\'Control settings for Annexin V experiments are critical to experimental results. It is strongly recommended to read It says \\\'this article before the experiment: https://www.elabscience.com/resource-cell_function-detail-984\\\' but I get a 404 warning from the site. Could you share the relevant file with me? You reply me: Click Annexin V-FITC/PI Apoptosis Kit to view: the answer. Bur when ı click the website you mentioned, I dont see both my question or response. Please share document via e-mail.
Reply
adminSubmitted [ Jun 06 2024 ]
Answered: Dear, Sorry for the problem brought by our website, you could refer to link as below: https://www.elabscience.com/List-detail-459717.html Regards,
g*********kSubmitted [ May 28 2024 ]
Asked: The protocol states \'Control settings for Annexin V experiments are critical to experimental results. It is strongly recommended to read It says \'this article before the experiment: https://www.elabscience.com/resource-cell_function-detail-984\' but I get a 404 warning from the site. Could you share the relevant file with me?
Reply
adminSubmitted [ May 28 2024 ]
Answered: Dear, Sorry for the inconvenience brought due to Elabscience website problem. You could refer to below link: https://www.elabscience.com/List-detail-459717.html Hope it will be helpful, for any other question, please let us know. Regards, Cathy