Click here to view cell apoptosis assay products

DAPI Reagent (1 μg/mL)


      Catalog number:E-IR-R103

      • 5mL
      • 10mL
      • 50mL
      - +
      Price: $20

      Lead Time:Order now, ship in 3 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual


      DAPI (4 ', 6-diamidino-2-phenylindole), is a kind of fluorescent dye which can strongly combine with DNA. It can be observed by fluorescence microscope. DAPI can penetrate the normal cell membrane, it can be used for staining of living cells or fixed cells. CAS number: 28718-90-3.

      DAPI can penetrate the cell membrane and combine with the double stranded DNA in the nucleus and produces more than 20 times stronger fluorescence than DAPI itself. It has stronger sensitivity to double stranded DNA staining than EB. Cells can be seen with blue fluorescence when DNA combine with DPAI by microscope. And the efficiency of cell labeling is high (almost 100%) without any effect on living cells. DAPI staining is often used to detect apoptosis. After staining, it is observed by fluorescence microscope or flow cytometry. DAPI is also commonly used in general nuclear staining and in some specific cases double stranded DNA staining. After heat shock treatment, the cells staining with DAPI for 3 min, and the morphological changes of nuclei could be seen by fluorescence microscope.

      The maximum excitation wavelength of DAPI is 340 nm, and the maximum emission wavelength is 488 nm. When DAPI combines with dsDNA, the maximum excitation wavelength is 360 nm, and the maximum emission wavelength is 460 nm. The DAPI staining solution can be directly used for nuclear staining of fixed cells or tissues.




      5 mL

      10 mL

      50 mL



      DAPI Reagent (1 μg/mL)

      5 mL

      10 mL

      50 mL


      Experimental Procedure

      1. For the fixed cell or tissue samples, wash and remove the fixative properly. If immunofluorescence staining is needed, it can be done first, then add DAPI to stain the nucleus. If no other staining is needed, DAPI staining is performed directly.

      2. For adherent cells or tissue sections, add a small amount of DAPI Reagent to cover the sample. For suspension cells, add at least 3 times volume of the sample and mix well to stain the nucleus.

      3. Incubate at room temperature for 5~10 min.

      4. Discard DAPI Reagent, wash with TBST, PBS or normal saline for 2~3 times, 3~5 min/time to wash off the unbinding DAPI.

      5. Observe the cells by fluorescence microscope with 360 nm excitation wavelength and 460 nm emission wavelength.


      Store at 2~8°C in dark. Avoid freezing. Valid for 12 months.


      1.  This product is irritating, please be careful when handling it and avoid direct contact with the human body or inhalation.

      2.  For your safety and health, please wear lab coats and disposable gloves for operation and follow the procedures of laboratory reagent operation.

      3.  Avoid extended exposure of the samples to direct light to protect the fluorophores from quenching.There are quenching problems

          in fluorescent dyes. It is recommended to observe the results as soon as possible after dyeing, or use the Anti-Fluorescence 

          Quenching Agent (E-IR-R119) to slow down the fluorescence quenching.


      1. Transient transfection of the respiratory epithelium with gamma interferon for host-directed therapy in pulmonary tuberculosis
        IF: 7.032
        Journal: Molecular Therapy-Nucleic Acids(2020)
        DOI: 10.1016/j.omtn.2020.10.023
        PMID: 33110704
        Products: E-IR-R103 
        Sample type: Cell sample
        Reactivity: Mouse
      2. Wound Healing Activity of the Flavonoid-Enriched Fraction of Selaginella bryopteris Linn. against Streptozocin-Induced Diabetes in Rats
        IF: 3.344
        Journal: Separations(2023)
        DOI: 10.3390/separations10030166
        Products: E-IR-R103 
      3. MiR-218 Promotes Adriamycin-Induced H9C2 Apoptosis by Inhibiting Stress-Associated Endoplasmic Reticulum Protein 1
        IF: 3.434
        Journal: DISEASE MARKERS(2022)
        DOI: 10.1155/2022/6881103
        PMID: 35027982
        Products: E-IR-R103 
      Show AllShorten
      • Show all (0)
      • Reviews (0)
      • Q&A (0)
      ... Show All Show Less
      MSDS for ELISA ISO 9001

      Browsing History

        People Also Bought

        Apply for
        *Product Name:
        *Catalog Number:
        *When will you use it?