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Cat.No.:E-AB-F0987M
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Alternate Names | Killer cell lectin-like receptor subfamily B member 1C, Klrb1c, CD161 antigen-like family member C, Ly-55c, NK1.1, NKR-P1.9, NKR-P1C, NKR-P1 40, CD161c |
Swissprot | P27814,P27812,Q99JB4 |
References |
1. Lanier LL. 1997. Immunity 6:371.
2. Yokoyama WM, et al. 1993. Ann. Rev. Immunol. 11:613. 3. Koo GC, et al. 1986. J. Immunol. 137:3742. 4. Giorda R, et al. 1991. J. Immunol. 147:1701. |
Form | Liquid |
Clone No. | PK136 |
Host | Mouse |
Isotype | Mouse IgG2a, κ |
Isotype Control | Elab Fluor® 647 Mouse IgG2a, κ Isotype Control[C1.18.4] [Product E-AB-F09802M] |
Reactivity | Mouse |
Application | FCM |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | Elab Fluor® 647 |
Elab Fluor® 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670 nm (e.g., a 660/20 nm bandpass filter). | |
How to select the appropriate detection channel through the spectrogram?
NK-1.1 surface antigen, also known as CD161b/CD161c and Ly-55, is encoded by the NKR-P1B/NKR-P1C gene. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |