Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> PE/Cyanine5.5 Anti-Mouse CD161/NK1.1 Antibody[PK136]
Cat.No.:E-AB-F0987UI
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Alternate Names | Killer cell lectin-like receptor subfamily B member 1C,Klrb1c,CD161 antigen-like family member C,Ly-55c,CD161/NK1.1,NKR-P1.9,NKR-P1C,NKR-P1 40,CD161c |
Swissprot | P27814,P27812,Q99JB4 |
References |
1. Lanier LL. 1997. Immunity 6:371.
2. Yokoyama WM, et al. 1993. Ann. Rev. Immunol. 11:613. 3. Koo GC, et al. 1986. J. Immunol. 137:3742. 4. Giorda R, et al. 1991. J. Immunol. 147:1701. |
Form | Liquid |
Clone No. | PK136 |
Host | Mouse |
Isotype | Mouse IgG2a, κ |
Isotype Control | PE/Cyanine5.5 Mouse IgG2a, κ Isotype Control[C1.18.4] [Product E-AB-F09803I] |
Reactivity | Mouse |
Application | FCM |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume]. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | PE/Cyanine5.5 |
PE/Cyanine5.5 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 690 nm (e.g., a 690/50 nm bandpass filter). | |
How to select the appropriate detection channel through the spectrogram?
NK-1.1 surface antigen, also known as CD161b/CD161c and Ly-55, is encoded by the NKR-P1B/NKR-P1C gene. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |