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MTT Cell Proliferation and Cytotoxicity Assay Kit

  • Cat.No.:E-CK-A341

To Purchase E-CK-A341

Size:
  • 500Assays
  • 1000Assays
Price: $136
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Detection principle

MTT is 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide which can be reduced by some dehydrogenases in mitochondria to form a crystalline dark purple product formazan. Formazan can be completely dissolved by DMSO and its absorbance is near 570 nm wavelength. The more and faster the cells proliferate, the darker the color is, and the more cytotoxic, the lighter the color is. For the same cells, there is a linear relationship between the depth darkness of color and the number of cells.

General Information

Application Viability/Proliferation/Cytotoxicity
Detection method Colorimetric Method
Sample type Cell samples
Detection instrument Microplate reader
Other reagents required DMSO
Storage 2~8°C, shading light
Shipping Ice bag
Expiration date 6 months

Citations

  1. SURFACE ENGINEERING (2020) IF: 2.433
    Ag-containing antibacterial self-healing micro-arc oxidation coatings on Mg–Zn–Sr alloys

    DOI: 10.1080/02670844.2020.1800346

    Sample: Cell Culture Supernatant
  2. NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL (2021) IF: 2.9
    Catechin Metabolites along with Curcumin Inhibit Proliferation and Induce Apoptosis in Cervical Cancer Cells by Regulating VEGF Expression In-Vitro

    DOI: 10.1080/01635581.2021.1936082

    PMID: 34121550

  3. Photodiagnosis and Photodynamic Therapy (2021) IF: 3.631
    Influence of antibacterial effects of tetracycline, laser, and photodynamic therapy on cell viability, cell damage, and virulence of Porphyromonas gingivalis.

    DOI: 10.1016/j.pdpdt.2021.102617

  4. BMC Complementary and Alternative Medicine (2022) IF: 3.659
    Evaluation of the antimalarial activity and toxicity of Mahanil-Tang-Thong formulation and its plant ingredients

    DOI: 10.1186/s12906-022-03531-2

    PMID: 35219319

  5. MOLECULES (2022) IF: 4.927
    Oncolytic Newcastle Disease Virus Co-Delivered with Modified PLGA Nanoparticles Encapsulating Temozolomide against Glioblastoma Cells: Developing an Effective Treatment Strategy

    DOI: 10.3390/molecules27185757

    PMID: 36144488

  6. MOLECULES (2023) IF: 4.927
    Enhanced Intracellular Photosensitizer Uptake and Retention by Targeting Viral Oncoproteins in Human Papillomavirus Infected Cancer Cells and Cancer Stem Cells

    DOI: 10.3390/molecules28020647

  7. FASEB JOURNAL (2020) IF: 4.966
    CaMK4‐dependent phosphorylation of Akt/mTOR underlies Th17 excessive activation in experimental autoimmune prostatitis

    DOI: 10.1096/fj.201902910RRR

    Sample: Cell
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