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Hexokinase (HK) Activity Assay Kit

Cat.No.:E-BC-K610-M
Detection instrument: Microplate reader (440-450 nm, optimum wavelength: 450 nm)

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96T

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Detection principle

HK converts glucose to glucose 6-phosphate, which is catalyzed by the enzyme. Meanwhile, NAD+ is reduced to NADH, which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of HK can be calculated by measuring the change of absorbance value at 450 nm.

Performance characteristics

Synonyms	HK
Sample type	animal tissue, cell
Sensitivity	5.93 U/L
Detection range	5.93-40.11 U/L
Assay type	Enzyme Activity
Assay time	30 min
Precision	Average inter-assay CV: 9.100%Average intra-assay CV: 3.300%
Other instruments required	Centrifuge, 37℃ incubator
Storage	-20℃
Valid period	12 months

Ralated product

Cat Number	Product Name
E-BC-K234-M	Glucose (Glu) Colorimetric Assay Kit (GOD-POD Method)
E-BC-K011-M	Glucose-6-phosphate (G6P) Colorimetric Assay Kit
E-BC-K056-M	Glucose-6-Phosphate Dehydrogenase (G-6-PD) Activity Assay Kit

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

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