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Immunol Fluorescence Staining Kit (Anti-Rabbit IgG-Cyanine3)


      Catalog number:E-IR-R321

      • 50 Assays
      • 100 Assays
      • 200 Assays
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      Price: $90

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      Immuno Fluorence Staining Kits are developed for immunofluorescence detection of cell or tissue sections. When there is an appropriate antibody to detect specific target protein, fluorescence can be detected by immunofluorescence staining kit.

      Immuno Fluorescence Staining Kit (Anti-Rabbit IgG-Cyanine3) contains Goat Anti-Rabbit IgG(H+L)(Cyanine3 conjugated), this secondary antibody can detect primary antibody from rabbit with bright red fluorescence.

      Cyanine3 is a newly red fluorescent probe. It is brighter than most of other red fluorescent probes, more difficult to quench, and has a lower background.

      The kit contains anti fluorescence quenching sealing solution, which can make the fluorescence more lasting.




      50 Assays

      100 Assays

      200 Assays



      Goat Anti-Rabbit IgG(H+L)( Cyanine3 conjugated)

      120 μL

      200 μL

      200 μL×2



      Normal Goat Blocking Buffer (Ready-to-Use)

      5 mL

      10 mL

      20 mL



      DAPI Reagent (1 μg/mL)

      5 mL

      10 mL

      20 mL



      Anti-Fluorescence Quenching Agent

      5 mL

      10 mL

      20 mL



      One copy

        Preparation of Immunofluorescence Staining

      A.   Preparation of Fixation Solution

      It is recommended to use 4% Paraformaldehyde as the fixation solution (E-IR-R113), or use ethanol, methanol or other types of fixative according to specific primary antibody or sample.

      B.   Preparation of Permeate working solution
      Use Triton X-100 as the permeable solution (E-IR-R122) and dilute with 1x PBS buffer to 0.5% Triton X-100 working solution.

      C.   Preparation of TBST Working Buffer

      It is recommended to use TBST as washing buffer. Use Elabscience ® 10× TBST (E-BC-R335) and dilute to 1 ×TBST Working Buffer with deionized water at ratio of 1:9.

      D.   Preparation of Antibody Dilution Solution

      It is recommended to use Elabscience ® Antibody Dilution Buffer (E-IR-R106) or PBS as primary antibody dilution buffer.

      E.   Dilute Primary Antibody

      Dilute the primary antibody according to the manual of primary antibody.

      F.   Dilute the Secondary Antibody

      It is recommended to use Elabscience ® Antibody Dilution Buffer (E-IR-R106) or PBS as secondary antibody dilution buffer. Dilute the secondary antibody with antibody dilution buffer at the dilution of 1:50. The dilution ratio can be increased or decreased appropriately according to the intensity of fluorescence.


      Store at 2~8/-20°C, Refer to the label. Valid for 12 months. Secondary antibody (E-AB-1010) and DAPI Reagent (E-IR-R103) should  be stored at -20°C and avoid light.


      1. This result should be observed by fluorescence microscope.

      2. Anti-Fluorescence Quenching Reagent can slow down the quenching, but avoid light and especially shorten the result observation time is still needed.

      3. If it can't be observed in time, please store the slice at 4°C and avoid light and observe in one week.

      4. If the fluorescence is too weak, increase the primary antibody concentration properly. If the fluorescence is still weak, increase  the secondary antibody concentration appropriately.

      5. The reagents for immunofluorescence staining and the cover glass and slides should be prepared in advance.

      6. For your safety and health, please wear the lab coat and disposable gloves before the experiments.


      1. TIPE2 May Target the Nrf2/HO-1 Pathway to Inhibit M1 Macrophage-Related Neutrophilic Inflammation in Asthma.
        IF: 7.561
        Journal: Frontiers in Immunology(2022)
        DOI: 10.3389/fimmu.2022.883885
        Products: E-IR-R321 
      2. RBM10 regulates alternative splicing of lncRNA Neat1 to inhibit the invasion and metastasis of NSCLC
        IF: 6.429
        Journal: Cancer Cell International(2022)
        DOI: 10.1186/s12935-022-02758-w
        Products: E-IR-R321 
      3. Cell line derived from muscle of Gymnocypris przewalskii, a species of Schizothoracinae in Qinghai Lake, Qinghai–Tibet Plateau
        IF: 2.723
        DOI: 10.1007/s11626-022-00729-z
        Products: E-IR-R321 
        Sample type: GPM cells
        Reactivity: Fish
      4. α‑rhamnrtin‑3‑α‑rhamnoside exerts anti‑inflammatory effects on lipopolysaccharide‑stimulated RAW264.7 cells by abrogating NF‑κB and activating the Nrf2 signaling pathway
        IF: 2.952
        Journal: Molecular Medicine Reports(2021)
        DOI: 10.3892/mmr.2021.12439
        PMID: 34523697
        Products: E-IR-R321 
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