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SDS-PAGE Gel kit

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      Catalog number:E-IR-R305

      Size:
      • 10 Assays
      • 25 Assays
      • 50 Assays
      Qty:
      - +
      Price: $36

      Lead Time:Order now, ship in 3 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Introduction

      Elabscience® SDS-PAGE Gel Assay kit is the classic SDS-PAGE gel preparation kit which contains all kinds of reagents needed to prepare the SDS-PAGE gel. Users only need apparatus and ddH2O to complete the gel preparation.

      The Gel Mix in this kit is the mixture of the gel buffer such as SDS and Tris-HCl and so on, which simplify the procedure of gel preparation. There are Tris-HCl pH 8.8 and SDS in the Separating Gel Mix, Tris-HCl pH 6.8 and SDS are in the Stacking Gel Mix.


      Compenonts



      Cat.

      Products

      10 Assays

      25 Assays

      50 Assays

      Storage

       E-IR-R305A

       30% Acr-Bis (29:1)

      45 mL

      100 mL

      100 mLx2

      2~8°C

       E-IR-R305B

       Separating Gel Mix

      22 mL

      55 mL

      55 mL x2

      RT

       E-IR-R305C

       Stacking Gel Mix

      6 mL

      12 mL

      22 mL

      RT

       E-IR-R305D

       APS

      170 mg

      170 mg x 2

      700 mg

      RT

       E-IR-R305E

       TEMED

      100 μL

      250 μL

      500 μL

      2~8°C

        Manual

       One Copy






      Instructions

      According to the different molecular weight of the target protein, different concentration of separating gel should be prepared.

      1. Comparison of protein separation linear range is shown in the manual.

      2. Sample volume of each component corresponding to different stacking gel volumes is shown in the manual.

      3. Sample volume of each component corresponding to different separation gel volumes is shown in the manual.


      Procedure

      1. Preparation of 10% APS: add distilled water or DI water (add 10μL of water to 1mg APS) to APS [E-IR-R305D] to prepare a 10% APS, 

          For example: add 1.7mL distilled water to170mg APS, the mixture is 10% APS. 10% APS should be split into small tubes and stored at -20°C.

      2. Choose the clean gel mold and complete the assembly.

      2.  Refer to in the manual, prepare the separating gel of the required concentration and volume. Add appropriate amount of ddH2O, 

           30% Acr-Bis (29:1)[ E-IR-R305A] and Separating Gel Mix [E-IR-R305B] to a clean beaker, mix it.

      3.  Add appropriate amount of 10% APS and TEMED, mix gently and avoid bubbles.

      4.  Add the mixture gel to the assembled gel mold immediately. Then add 1-2 mL ddH2O or absolute alcohol on the separating gel to

           flatten the separating gel level.

      5.  Keep at RT for 30-60 min until the separating gel solidified completely.

      6.  Pour out the ddH2O or absolute ethanol, absorb the residual liquid with absorbent paper.

      7.  Refer to in the manual, prepare the stacking gel of the required volume. Add appropriate amount of ddH2O, 30% Acr-Bis (29:1)

           [ E-IR-R305A] and stacking Gel Mix [E-IR-R305C] to a clean beaker, mix it.

      8.  Add appropriate amount of 10% APS and TEMED, mix gently and avoid bubbles.

      9.  Add the mixture gel upon to the separating gel immediately. Insert comb teeth carefully, avoid bubbles.

      10. Keep at RT for 30-40 min until the stacking gel solidified completely.

      11. Pull out the comb teeth carefully. Follow the SDS-PAGE experiment.

       

      Storage

      Store separately.

      30% Acr-Bis (29:1) [E-IR-R305A] and TEMED[E-IR-R305E] should be stored at 2~8°C in dark.

      10% APS should be split into small tubes and stored at -20°C for 6 months, If store at 2~8°C, please do not use it after one week.


      Other reagents should be stored at RT.


      Cautions

      1. For maximal assay performance, this reagent should be used within 6 months. Avoid freeze / thaw cycles.

      2. TEMED is volatile. Please tighten the cap after use. Due to the different temperatures, the rate of gel solidification will be different. The amount of TEMED can be adjusted appropriately to adjust the rate of gelation at different temperatures.

      3.  Avoid bubbles to avoid affecting the experimental results.

      4.  Add the ddH2O or absolute alcohol on the separating gel gently to avoid uneven line pressing.

      5.  This kit is for research use only. For your safety and health, please wear lab clothes and gloves. Instructions should be followed strictly, changes of operation may result in unreliable results. 

      Citations

      1. Silencing of circular RNA circPDE5A suppresses neuroblastoma progression by targeting the miR-362-5p/NOL4L axis
        IF: 2.292
        Journal: INTERNATIONAL JOURNAL OF NEUROSCIENCE(2021)
        DOI: 10.1080/00207454.2021.1896505
        PMID: 33635749
        Products: E-IR-R305 
        Sample type: Tissue Homogenate(Neuroblastoma),Cell Lysate(Nb Ce
        Reactivity: Human
      2. Overexpression of Long Non-Coding RNA MIR22HG Represses Proliferation and Enhances Apoptosis via miR-629-5p/TET3 Axis in Osteosarcoma Cells
        IF: 2.351
        Journal: JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY(2021)
        DOI: 10.4014/jmb.2106.06028
        PMID: 34373436
        Products: E-IR-R305 
      3. Protective effects of endothelial progenitor cell microvesicles carrying miR‑98‑5p on angiotensin II‑induced rat kidney cell injury
        IF: 2.751
        Journal: Experimental and Therapeutic Medicine(2022)
        DOI: 10.3892/etm.2022.11638
        PMID: 36277153
        Products: E-IR-R305 
      4. Lactobacillus paracasei from Koumiss Ameliorates Diarrhea in mice via Tight Junctions Modulation
        IF: 4.008
        Journal: NUTRITION(2022)
        DOI: 10.1016/j.nut.2021.111584
        PMID: 35395579
        Products: E-IR-R305 
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