Western Blot(WB) Related Reagents
Western Blot (WB) is one of the most conventional biological experiments. SDS-PAGE gel is used to transfer the separated proteins to the membrane (such as PVDF membrane). Then, the target protein is detected by the specific antibody that recognizes the target protein, and the specific antibody is recognized by the labeled second antibody. The technique is widely used to detect the expression of target protein level.
As we all know, there are many kinds of WB related reagents in the market. How to find a reliable and stable supplier? What reagents are needed for a complete set of WB experiments? Elabscience® provides you with one-stop WB experiment related reagents, which meet the needs of your various process experiments and solve your worries.
Click the process below to select the reagent products that meet your experimental requirements!
- All
- WB Kits
- Sample Preparation
- Concentration Detection
- SDS-PAGE Gel
- Electrophoresis
- Staining
- Transfer
- Blocking
- Dilution
- Secondary AB
- ECL
- Buffer
| Cat.No. | Product Name | Size | Price | Application |
|---|---|---|---|---|
| E-IR-R304A | Western Blot Detection Kit (PVDF membrane, 0.45 μm) | 50 Assays | $210 | WB |
| E-IR-R304B | Western Blot Detection Kit (PVDF membrane, 0.22 μm) | 50 Assays | $230 | WB |
| E-BC-R327 | RIPA Lysis Buffer | 20mL/50mL/100mL | $25/60/80 | Sample Preparation |
| E-BC-R288 | 5×SDS Loading Buffer | 2mL/5mL/10mL | $20/25/40 | Sample Preparation |
| E-BC-K318 | Total Protein (TP) Colorimetric Assay Kit (BCA Method) | 96T / 500Assays | $100/200 | Concentration Detection |
| E-IR-R305 | SDS-PAGE Gel kit | 10Assays / 25Assays / 50Assays | $36/60/96 | SDS-PAGE |
| E-BC-R273 | Pre-stained Protein Marker(10-180kDa) | 250μL/500μL/2.5mL | $150/250/900 | Electrophoresis |
| E-BC-R331 | Electrophoresis Buffer (10 ×) | 100mL/200mL/500mL | $15/20/25 | Electrophoresis |
| E-IR-R127 | Ponceau S Solution | 100mL/200mL/500mL | $20/30/50 | Staining |
| E-IR-R129 | Fast Coomassie Blue Staining Solution(10×) | 100mL/200mL | $35/50 | Staining |
| E-BC-R333 | Transmembrane Buffer (10×) | 100mL/200mL/500mL | $10/15/20 | Transfer |
| E-BC-R266 | PVDF Membrane(0.45μm) | 8.5cm×6cm×10pieces/20pieces | $100/160 | Transfer |
| 13.25cm×15cm×10pieces/20pieces | $280/450 | Transfer | ||
| E-BC-R329 | PVDF Membrane(0.22μm) | 8.5cm×6cm×10pieces/20pieces | $145/229 | Transfer |
| 13.25 cm×15 cm×10 pieces/20 pieces | $440/640 | Transfer | ||
| E-BC-R337 | Skim Milk Powder | 5g/10g/50g | $10/18/30 | Blocking |
| E-IR-R108 | BSA | 5g/10g/50g | $60/100/400 | Blocking |
| E-IR-R107 | 5% BSA Blocking Buffer | 5mL/10mL/50mL | $8/10/30 | Blocking |
| E-IR-R121 | Western Blotting Antibody Dilution Buffer | 100mL/200mL/500mL | $40/70/120 | Dilution |
| E-IR-R125 | Western Antibody Dilution Buffer (Block Quickly) | 100mL/200mL/500mL | $50/80/150 | Dilution |
| E-AB-1001 | Goat Anti-Mouse IgG(H+L)-HRP | 60μL/120μL/500μL | $21/34/100 | Secondary AB |
| E-AB-1003 | Goat Anti-Rabbit IgG(H+L)-HRP | 60μL/120μL/500μL | $21/34/100 | Secondary AB |
| E-AB-1004 | Rabbit Anti-Goat IgG(H+L)-HRP | 60μL/120μL/500μL | $21/34/100 | Secondary AB |
| E-IR-R307 | Excellent Chemiluminescent Substrate（ECL）Detection Kit | 50mL/100mL/500mL | $50/80/200 | WB,ELISA |
| E-IR-R308 | Super Excellent Chemiluminescent Substrate（ECL）Detection Kit | 50mL/100mL/500mL | $60/90/220 | WB,ELISA |
| E-BC-R187 | PBS Buffer, pH7.4（10×） | 100mL/200mL/500mL | $10/15/22 | Buffer |
| E-BC-R335 | TBST Buffer (10×) | 100mL/200mL/500mL | $10/15/20 | Buffer |
| E-IR-R102 | Western Blot Stripping Buffer (Neutral) | 100mL/200mL/500mL | $30/50/100 | Buffer |
| E-IR-R116 | 10×TBS(pH7.4) | 100mL/200mL/500mL | $10/15/20 | Buffer |
| E-IR-R310 | PBST Buffer, pH7.4 (10 ×) | 500mL | $25 | Buffer |
Western Blot Kit (E-IR-R304)
Western Blot (WB) is the most common and classical experimental method for detecting protein expression in the biological science. However, due to differences in reagent sources, methods of operation and experience used by different Laboratory Technician, a large number of WB experimental results do not meet the requirement of paper publishing. Elabscience has been focusing on the field of immunology for many years and has accumulated a lot of experience in WB operation. In order to help researchers solve the above problems, the Western Blot kit is now available. The reagents in this kit are provided for classical WB experiments, including all reagents needed from protein extraction to result detection, which have advantages of simple operation, high detection sensitivity, low background and strong system stability. And Elabscience provides detailed operation description and professional technical support. Additionally, it offers you convenience and guarantee to work out ideal Western Blot experiment results.
As the PVDF membranes have two pore sizes for different molecular weights of target proteins respectively. In order to meet different experimental requirements, we have designed two specifications of Western Blot kits.
E-IR-R304A (PVDF membrane, 0.45 μm) is recommended for the proteins detection with molecular weights more than 20 kDa.
E-IR-R304B (PVDF membrane, 0.22 μm) is recommended for the proteins detection with molecular weights less than 20 kDa.
- E-IR-R304A
- E-IR-R304B
E-IR-R304A (PVDF membrane, 0.45 μm) is recommended for the proteins detection with molecular weights more than 20 kDa.
Provided product
| Product name | Cat.No. | Size | Storage | Shelf life |
|---|---|---|---|---|
| RIPA Lysis Buffer (with PMSF and Na3VO4) | E-BC-R327 | 5 mL/50 μL+50 μL | -20℃ | 6 months |
| Total Protein Colorimetric Assay Kit (BCA method) | E-BC-K318 | 48 T | 4℃/-20℃ | 6 months |
| Excellent Chemiluminescent Substrate Detection Kit | E-BC-R347 | 15 mL/15 mL | 4℃ | 6 months |
| Pre-stained Protein Marker (10-180 kDa) | E-BC-R273 | 25 µL | -20℃ | 12 months |
| Goat Anti-Rabbit IgG(H+L)-HRP | E-AB-1003 | 20 µL | -20℃ | 12 months |
| Skim Milk Powder | E-BC-R337 | 15 g | RT | 6 months |
| PBS Buffer, pH7.4 (10 ×) | E-BC-R187 | 100 mL | RT | 6 months |
| 5 × SDS Loading Buffer | E-BC-R288 | 1 mL × 2 | -20℃ | 6 months |
| Electrophoresis Buffer (10 ×) | E-BC-R331 | 125 mL × 2 | RT | 6 months |
| Transmembrane Buffer (10 ×) | E-BC-R333 | 125 mL × 2 | 4℃ | 6 months |
| TBST Buffer (10 ×) | E-BC-R335 | 125 mL × 2 | 2~8°C | 12 months |
| PVDF Membrane (0.45 μm, 8.5 × 6 cm) | E-BC-R266 | 5 pieces | RT | 12 months |
Alternative Reagent offer
| Product name | Cat.No. | Size | Storage | Shelf life |
|---|---|---|---|---|
| Goat Anti-Mouse IgG(H+L)-HRP | E-AB-1001 | 20 µL | -20℃ | 12 months |
Self-Prepared Reagent:Methanol
Instructions for user
This kit is for five gels (about 50 samples) with the Western Blot experiment.
Please store the reagents according to the storage conditions provided in the manual.
Message submitted successfully !