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Super Excellent Chemiluminescent Substrate (ECL)Detection Kit

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      Catalog number:E-IR-R308

      Size:
      • 50 mL
      • 100 mL
      • 500 mL
      Qty:
      - +
      Price: $60

      Lead Time:Order now, ship in 3 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Introduction

      Elabscience ® Super Excellent Chemiluminescent SubstrateECLDetection Kit is a horseradish peroxidase (HRP) substrate, which can be used to detect horseradish peroxidase (HRP) labeled proteins or nucleic acids, analyze protein content by Western blot or ELISA, and is compatible with film and digital development system.

      The special ingredients contained in this product can effectively reduce non-specific luminescence and background luminescence, even if exposed for a long time, the background is still extremely low.

      The signal of this product decays slowly, and the luminescence is not significantly weakened after 30 min, and the luminous intensity can still reach about 70% of the highest luminous intensity after 120 min.



      Components

      Cat. Products 50 mL 100 mL 500 mL Storage
      E-IR-R308A Super ECL Substrate A 25 mL 50 mL 250 mL 2~8°C
      E-IR-R308B Super ECL Substrate B 25 mL 50 mL 250 mL 2~8°C


      Instructions

      1.  Western Blot

      1)  Follow the step of Western Blotting to block the membrane and incubate the primary/secondary antibody.

      2)  Wash the membrane with TBST for 3 times, 5 min each time.

      3)  Prepare ECL Working Solution by mixing equal parts of the ECL Substrate A and ECL Substrate B.

      Note: Prepare the working solution before use, and change the pipette tips during the suction process.

      4)  Take an appropriate amount of mixed ECL Working Solution and add it to the membrane.

      5)  Adjust the exposure time according to the intensity of the luminous intensity

      2.  ELISA

      1)  Prepare Working Solution by mixing equal parts of the ECL Substrate A and ECL Substrate B.

      Note: Prepare the working solution before use, and change the pipette tips during the suction process.

      2)  Add 50~200 μL mixed ECL Working Solution to each well into the microtiter plate.

      3)  Detection within 10~60 min, better within 40~50 min.



      Storage

      Store at 2~8°C for 12 months.


      Cautions

      1. Before using this product, the imprinting film must be thoroughly washed, otherwise it may cause the background to rise.

      2. ECL Substrate A and ECL Substrate B must be prepared before use, and change the pipette tips during the suction process.

      3. Do not expose ECL working solution to sunlight or strong light, otherwise it will cause its inactivation, and laboratory light will have little effect on the working solution.

      4. NaN3 inhibits HRP activity, NaN3 should be avoided during the experiment.


      Citations

      1. FABP4-mediated homocysteine-induced cholesterol accumulation in THP-1 monocyte-derived macrophages and the potential epigenetic mechanism
        IF: 1.559
        Journal: Molecular Medicine Reports(2016)
        DOI: 10.3892/mmr.2016.5315
        Products: E-IR-R307 E-IR-R308 
      2. The Role of IL-6 and TMEM100 in Lumbar Discogenic Pain and the Mechanism of the Glycine-Serine-Threonine Metabolic Axis: A Metabolomic and Molecular Biology Study
        IF: 2.832
        Journal: Journal of Pain Research(2023)
        DOI: 10.2147/JPR.S400871
        Products: E-IR-R307 E-IR-R308 
      3. Diosmectite inhibits the interaction between SARS-CoV-2 and human enterocytes by trapping viral particles, thereby preventing NF-kappaB activation and CXCL10 secretion
        IF: 4.38
        Journal: Scientific Reports(2021)
        DOI: 10.1038/s41598-021-01217-2
        PMID: 34741071
        Products: E-IR-R307 E-IR-R308 
        Sample type: Caco-2 Cells
        Reactivity: Human
      4. Simultaneous reinforcement and toughening of polymer/hydroxyapatite composites by constructing bone-like structure
        IF: 4.873
        Journal: COMPOSITES SCIENCE AND TECHNOLOGY(2017)
        DOI: 10.1016/j.compscitech.2017.08.026
        Products: E-IR-R307 E-IR-R308 
      5. Isoliquiritigenin alleviates P. gingivalis-LPS/ATP-induced pyroptosis by inhibiting NF-κB/ NLRP3/GSDMD signals in human gingival fibroblasts
        IF: 4.932
        Journal: INTERNATIONAL IMMUNOPHARMACOLOGY(2021)
        DOI: 10.1016/j.intimp.2021.108338
        PMID: 34794890
        Products: E-IR-R307 E-IR-R308 
        Sample type: Hgfs Cells
        Reactivity: Human
      6. Non-covalent strategies to functionalize polymeric nanoparticles with NGR peptides for targeting breast cancer
        IF: 6.51
        Journal: INTERNATIONAL JOURNAL OF PHARMACEUTICS(2023)
        DOI: 10.1016/j.ijpharm.2023.122618
        Products: E-IR-R307 E-IR-R308 
      7. Hyperbaric oxygen attenuates neuropathic pain and reverses inflammatory signaling likely via the Kindlin-1/Wnt-10a signaling pathway in the chronic pain injury model in rats
        IF: 7.277
        Journal: JOURNAL OF HEADACHE AND PAIN(2017)
        DOI: 10.1186/s10194-016-0713-y
        Products: E-IR-R307 E-IR-R308 
      8. Muscle Oxidative Stress Plays a Role in Hyperthyroidism-Linked Insulin Resistance
        IF: 7.675
        Journal: Antioxidants(2023)
        DOI: 10.3390/antiox12030592
        Products: E-IR-R307 E-IR-R308 
      9. Age-Dependent Skeletal Muscle Mitochondrial Response to Short-Term Increased Dietary Fructose
        IF: 7.675
        Journal: Antioxidants(2023)
        DOI: 10.3390/antiox12020299
        Products: E-IR-R307 E-IR-R308 
      10. Inter-Organelle Contact Sites Mediate the Intracellular Antioxidant Activity of Platinum Nanozymes: A New Perspective on Cell–Nanoparticle Interaction and Signaling
        IF: 10.383
        Journal: ACS Applied Materials & Interfaces(2023)
        DOI: 10.1021/acsami.2c22375
        Products: E-IR-R307 E-IR-R308 
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