Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Annexin V-labeled fluorescein bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscope. Nuclear dyes (PI, DAPI, 7-AAD) have a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. Due to late apoptosis or loss of membrane integrity in necrotic cells, nuclear dyes can enter cells to stain DNA, and when used in combination with Annexin V, cells in different apoptotic stages can be distinguished.

General Information

Application	Annexin V
Detection method	Fluorometric Method
Sample type	Cell samples
Assay time	40min
Detection instrument	Flow Cytometer
Dye Type	APC
Ex/Em (nm)	650/660
Channel set	APC
Other reagents required	PBS(E-BC-R187), Deionized water
Storage	2~8°C, shading light.
Shipping	Ice bag
Expiration date	12 months

Related Products

E-CK-A151	Annexin V Binding Buffer (10×)
E-CK-A117	Annexin V-APC Reagent
E-CK-A217	Annexin V-APC/PI Apoptosis Kit
E-CK-A258	Annexin V-APC/DAPI Apoptosis Kit
E-CK-A320	One-step TUNEL In Situ Apoptosis Kit (Green, FITC)
E-CK-A301	Mitochondrial Membrane Potential Assay Kit (with JC-1)

Citations

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023) IF: 6.208

METTL3 Regulates Osteoclast Biological Behaviors via iNOS/NO-Mediated Mitochondrial Dysfunction in Inflammatory Conditions

DOI: 10.3390/ijms24021403

TISSUE & CELL (2022) IF: 2.466

Upregulation of BRD7 protects podocytes against high glucose-induced apoptosis by enhancing Nrf2 in a GSK-3β-dependent manner

DOI: 10.1016/j.tice.2022.101813
PMID: 35550209

TISSUE & CELL (2022) IF: 2.466

Deactivation of AKT/GSK-3β-mediated Wnt/β-catenin pathway by silencing of KIF26B weakens the malignant behaviors of non-small cell lung cancer

DOI: 10.1016/j.tice.2022.101750
PMID: 35182988

TISSUE & CELL (2022) IF: 2.466

Activation of YAP1 by STK25 contributes to the progression of hepatocellular carcinoma

DOI: 10.1016/j.tice.2022.101797
PMID: 35421669

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2022) IF: 3.322

CAMK2N1 has a cancer-suppressive function in colorectal carcinoma via effects on the Wnt/β-catenin pathway

DOI: 10.1016/j.bbrc.2022.08.036
PMID: 35998547

JOURNAL OF NEUROIMMUNOLOGY (2021) IF: 3.478

Hydroxychloroquine alleviates the neurotoxicity induced by anti-ribosomal P antibodies

DOI: 10.1016/j.jneuroim.2021.577648
PMID: 34229206

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2022) IF: 3.575

Mesenchyme homeobox 2 has a cancer-inhibiting function in breast carcinoma via affection of the PI3K/AKT/mTOR and ERK1/2 pathways

DOI: 10.1016/j.bbrc.2022.01.011
PMID: 35051778

METABOLIC BRAIN DISEASE (2022) IF: 3.655

CDGSH iron sulfur domain 2 mitigates apoptosis, oxidative stress and inflammatory response caused by oxygen–glucose deprivation/reoxygenation in HT22 hippocampal neurons by Akt-Nrf2-activated pathway

DOI: 10.1007/s11011-022-01043-z

FOOD AND CHEMICAL TOXICOLOGY (2019) IF: 3.775

pH sensitive doxorubicin-loaded nanoparticle based on Radix pseudostellariae protein-polysaccharide conjugate and its improvement on HepG2 cellular uptake of doxorubicin

DOI: 10.1016/j.fct.2019.111099

ENVIRONMENTAL TOXICOLOGY (2022) IF: 4.119

Suppression of rhomboid domain-containing 1 produces anticancer effects in pancreatic adenocarcinoma through affection of the AKT/GSK-3β/β-catenin pathway

DOI: 10.1002/tox.23541
PMID: 35442567

ENVIRONMENTAL TOXICOLOGY (2021) IF: 4.119

Angiomotin-like 1 plays a tumor-promoting role in glioma by enhancing the activation of YAP1 signaling

DOI: 10.1002/tox.23363
PMID: 34480788

TOXICOLOGY AND APPLIED PHARMACOLOGY (2022) IF: 4.219

TRIM62 silencing represses the proliferation and invasion and increases the chemosensitivity of hepatocellular carcinoma cells by affecting the NF-κB pathway

DOI: 10.1016/j.taap.2022.116035
PMID: 35469851

TOXICOLOGY AND APPLIED PHARMACOLOGY (2021) IF: 4.219

TMED3 exerts a protumor function in non-small cell lung cancer by enhancing the Wnt/β-catenin pathway via regulation of AKT

DOI: 10.1016/j.taap.2021.115793
PMID: 34758370

TOXICOLOGY AND APPLIED PHARMACOLOGY (2022) IF: 4.219

Silencing of Nudix type 5 represses proliferation and invasion and enhances chemosensitivity of gastric carcinoma cells by affecting the AKT/GSK-3β/β-catenin pathway

DOI: 10.1016/j.taap.2022.115968
PMID: 35247377

TOXICOLOGY AND APPLIED PHARMACOLOGY (2022) IF: 4.219

Restraint of chaperonin containing T-complex protein-1 subunit 3 has antitumor roles in non-small cell lung cancer via affection of YAP1

DOI: 10.1016/j.taap.2022.115926
PMID: 35182550

TOXICOLOGY AND APPLIED PHARMACOLOGY (2021) IF: 4.219

Kinesin family member 23 exerts a protumor function in breast cancer via stimulation of the Wnt/β-catenin pathway

DOI: 10.1016/j.taap.2021.115834
PMID: 34933054

CELLULAR SIGNALLING (2022) IF: 4.315

METTL3 mediates osteoblast apoptosis by regulating endoplasmic reticulum stress during LPS-induced inflammation

DOI: 10.1016/j.cellsig.2022.110335
PMID: 35461899

TOXICOLOGY AND APPLIED PHARMACOLOGY (2022) IF: 4.46

Zinc finger protein 671 has a cancer-inhibiting function in colorectal carcinoma via the deactivation of Notch signaling

DOI: 10.1016/j.taap.2022.116326

TOXICOLOGY AND APPLIED PHARMACOLOGY (2022) IF: 4.46

The inhibition of centromere protein K causes anticancer effects in breast carcinoma via effects on the FAK/PI3K/AKT/mTOR pathway

DOI: 10.1016/j.taap.2022.116232
PMID: 36089000

TOXICOLOGY AND APPLIED PHARMACOLOGY (2022) IF: 4.46

Inhibition of PTPN21 has antitumor effects in glioma by restraining the EGFR/PI3K/AKT pathway

DOI: 10.1016/j.taap.2022.116180
PMID: 35907586

INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 4.932

Perilipin 5 protects against oxygen-glucose deprivation/reoxygenation-elicited neuronal damage by inhibiting oxidative stress and inflammatory injury via the Akt-GSK-3β-Nrf2 pathway

DOI: 10.1016/j.intimp.2022.108718
PMID: 35367744

MOLECULAR NEUROBIOLOGY (2021) IF: 5.59

Lipin1 Alleviates Autophagy Disorder in Sciatic Nerve and Improves Diabetic Peripheral Neuropathy

DOI: 10.1007/s12035-021-02540-5

INTERNATIONAL IMMUNOPHARMACOLOGY (2023) IF: 5.714

C1q/tumor necrosis factor-related protein-6 exerts protective effects on myocardial ischemia–reperfusion injury through the modulation of the Akt-GSK-3β-Nrf2 signaling cascade

DOI: 10.1016/j.intimp.2023.109678

Frontiers in Oncology (2022) IF: 5.738

PRKCI Mediates Radiosensitivity via the Hedgehog/GLI1 Pathway in Cervical Cancer

DOI: 10.3389/fonc.2022.887139
PMID: 35785194

International Journal of Nanomedicine (2022) IF: 6.4

Self-Assembled Nano-Peptide Hydrogels with Human Umbilical Cord Mesenchymal Stem Cell Spheroids Accelerate Diabetic Skin Wound Healing by Inhibiting Inflammation and Promoting Angiogenesis

DOI: 10.2147/IJN.S363777

Pharmaceutics (2022) IF: 6.525

Preparation of DSPE-PEG-cRGD Modified Cationic Liposomes for Delivery of OC-2 shRNA and the Antitumor Effects on Breast Cancer

DOI: 10.3390/pharmaceutics14102157
PMID: 36297592

INFLAMMATION RESEARCH (2022) IF: 6.986

Loss of pleckstrin homology domain and leucine-rich repeat protein phosphatase 2 has protective effects on high glucose-injured retinal ganglion cells via the effect on the Akt–GSK–3β–Nrf2 pathway

DOI: 10.1007/s00011-022-01680-1

Oxidative Medicine and Cellular Longevity (2023) IF: 7.31

Overexpression of PRDX2 in Adipose-Derived Mesenchymal Stem Cells Enhances the Therapeutic Effect in a Neurogenic Erectile Dysfunction Rat Model by Inhibiting Ferroptosis

DOI: 10.1155/2023/4952857
	Sample: Adipose-Derived Mesenchymal Stem Cells (Adscs)

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Annexin V-APC/7-AAD Apoptosis Kit

To Purchase E-CK-A218

Detection principle

General Information

Related Products

Citations

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023) IF: 6.208

TISSUE & CELL (2022) IF: 2.466

TISSUE & CELL (2022) IF: 2.466

TISSUE & CELL (2022) IF: 2.466

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2022) IF: 3.322

JOURNAL OF NEUROIMMUNOLOGY (2021) IF: 3.478

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2022) IF: 3.575

METABOLIC BRAIN DISEASE (2022) IF: 3.655

FOOD AND CHEMICAL TOXICOLOGY (2019) IF: 3.775

ENVIRONMENTAL TOXICOLOGY (2022) IF: 4.119

ENVIRONMENTAL TOXICOLOGY (2021) IF: 4.119

TOXICOLOGY AND APPLIED PHARMACOLOGY (2022) IF: 4.219

TOXICOLOGY AND APPLIED PHARMACOLOGY (2021) IF: 4.219

TOXICOLOGY AND APPLIED PHARMACOLOGY (2022) IF: 4.219

TOXICOLOGY AND APPLIED PHARMACOLOGY (2022) IF: 4.219

TOXICOLOGY AND APPLIED PHARMACOLOGY (2021) IF: 4.219

CELLULAR SIGNALLING (2022) IF: 4.315

TOXICOLOGY AND APPLIED PHARMACOLOGY (2022) IF: 4.46

TOXICOLOGY AND APPLIED PHARMACOLOGY (2022) IF: 4.46

TOXICOLOGY AND APPLIED PHARMACOLOGY (2022) IF: 4.46

INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 4.932

MOLECULAR NEUROBIOLOGY (2021) IF: 5.59

INTERNATIONAL IMMUNOPHARMACOLOGY (2023) IF: 5.714

Frontiers in Oncology (2022) IF: 5.738

International Journal of Nanomedicine (2022) IF: 6.4

Pharmaceutics (2022) IF: 6.525

INFLAMMATION RESEARCH (2022) IF: 6.986

Oxidative Medicine and Cellular Longevity (2023) IF: 7.31

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