Click here to view cell apoptosis assay products
×

Annexin V-APC/7-AAD Apoptosis Kit

  • Cat.No.:E-CK-A218

To Purchase E-CK-A218

Size:
  • 20Assays
  • 50Assays
  • 100Assays
  • 200Assays
Price: $75
Qty:

Detection principle

Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Annexin V-labeled fluorescein bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscope. Nuclear dyes (PI, DAPI, 7-AAD) have a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. Due to late apoptosis or loss of membrane integrity in necrotic cells, nuclear dyes can enter cells to stain DNA, and when used in combination with Annexin V, cells in different apoptotic stages can be distinguished.

General Information

Application Annexin V
Detection method Fluorometric Method
Sample type Cell samples
Assay time 40min
Detection instrument Flow Cytometer
Dye Type APC
Ex/Em (nm) 650/660
Channel set APC
Other reagents required PBS(E-BC-R187), Deionized water
Storage 2~8°C, shading light.
Shipping Ice bag
Expiration date 12 months

Citations

  1. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023) IF: 6.208
    METTL3 Regulates Osteoclast Biological Behaviors via iNOS/NO-Mediated Mitochondrial Dysfunction in Inflammatory Conditions

    DOI: 10.3390/ijms24021403

  2. TISSUE & CELL (2022) IF: 2.466
    Upregulation of BRD7 protects podocytes against high glucose-induced apoptosis by enhancing Nrf2 in a GSK-3β-dependent manner

    DOI: 10.1016/j.tice.2022.101813

    PMID: 35550209

  3. TISSUE & CELL (2022) IF: 2.466
    Deactivation of AKT/GSK-3β-mediated Wnt/β-catenin pathway by silencing of KIF26B weakens the malignant behaviors of non-small cell lung cancer

    DOI: 10.1016/j.tice.2022.101750

    PMID: 35182988

  4. TISSUE & CELL (2022) IF: 2.466
    Activation of YAP1 by STK25 contributes to the progression of hepatocellular carcinoma

    DOI: 10.1016/j.tice.2022.101797

    PMID: 35421669

  5. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2022) IF: 3.322
    CAMK2N1 has a cancer-suppressive function in colorectal carcinoma via effects on the Wnt/β-catenin pathway

    DOI: 10.1016/j.bbrc.2022.08.036

    PMID: 35998547

  6. JOURNAL OF NEUROIMMUNOLOGY (2021) IF: 3.478
    Hydroxychloroquine alleviates the neurotoxicity induced by anti-ribosomal P antibodies

    DOI: 10.1016/j.jneuroim.2021.577648

    PMID: 34229206

  7. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2022) IF: 3.575
    Mesenchyme homeobox 2 has a cancer-inhibiting function in breast carcinoma via affection of the PI3K/AKT/mTOR and ERK1/2 pathways

    DOI: 10.1016/j.bbrc.2022.01.011

    PMID: 35051778

  8. METABOLIC BRAIN DISEASE (2022) IF: 3.655
    CDGSH iron sulfur domain 2 mitigates apoptosis, oxidative stress and inflammatory response caused by oxygen–glucose deprivation/reoxygenation in HT22 hippocampal neurons by Akt-Nrf2-activated pathway

    DOI: 10.1007/s11011-022-01043-z

  9. FOOD AND CHEMICAL TOXICOLOGY (2019) IF: 3.775
    pH sensitive doxorubicin-loaded nanoparticle based on Radix pseudostellariae protein-polysaccharide conjugate and its improvement on HepG2 cellular uptake of doxorubicin

    DOI: 10.1016/j.fct.2019.111099

  10. ENVIRONMENTAL TOXICOLOGY (2022) IF: 4.119
    Suppression of rhomboid domain-containing 1 produces anticancer effects in pancreatic adenocarcinoma through affection of the AKT/GSK-3β/β-catenin pathway

    DOI: 10.1002/tox.23541

    PMID: 35442567

  11. ENVIRONMENTAL TOXICOLOGY (2021) IF: 4.119
    Angiomotin-like 1 plays a tumor-promoting role in glioma by enhancing the activation of YAP1 signaling

    DOI: 10.1002/tox.23363

    PMID: 34480788

  12. TOXICOLOGY AND APPLIED PHARMACOLOGY (2022) IF: 4.219
    TRIM62 silencing represses the proliferation and invasion and increases the chemosensitivity of hepatocellular carcinoma cells by affecting the NF-κB pathway

    DOI: 10.1016/j.taap.2022.116035

    PMID: 35469851

  13. TOXICOLOGY AND APPLIED PHARMACOLOGY (2021) IF: 4.219
    TMED3 exerts a protumor function in non-small cell lung cancer by enhancing the Wnt/β-catenin pathway via regulation of AKT

    DOI: 10.1016/j.taap.2021.115793

    PMID: 34758370

  14. TOXICOLOGY AND APPLIED PHARMACOLOGY (2022) IF: 4.219
    Silencing of Nudix type 5 represses proliferation and invasion and enhances chemosensitivity of gastric carcinoma cells by affecting the AKT/GSK-3β/β-catenin pathway

    DOI: 10.1016/j.taap.2022.115968

    PMID: 35247377

  15. TOXICOLOGY AND APPLIED PHARMACOLOGY (2022) IF: 4.219
    Restraint of chaperonin containing T-complex protein-1 subunit 3 has antitumor roles in non-small cell lung cancer via affection of YAP1

    DOI: 10.1016/j.taap.2022.115926

    PMID: 35182550

  16. TOXICOLOGY AND APPLIED PHARMACOLOGY (2021) IF: 4.219
    Kinesin family member 23 exerts a protumor function in breast cancer via stimulation of the Wnt/β-catenin pathway

    DOI: 10.1016/j.taap.2021.115834

    PMID: 34933054

  17. CELLULAR SIGNALLING (2022) IF: 4.315
    METTL3 mediates osteoblast apoptosis by regulating endoplasmic reticulum stress during LPS-induced inflammation

    DOI: 10.1016/j.cellsig.2022.110335

    PMID: 35461899

  18. TOXICOLOGY AND APPLIED PHARMACOLOGY (2022) IF: 4.46
    Zinc finger protein 671 has a cancer-inhibiting function in colorectal carcinoma via the deactivation of Notch signaling

    DOI: 10.1016/j.taap.2022.116326

  19. TOXICOLOGY AND APPLIED PHARMACOLOGY (2022) IF: 4.46
    The inhibition of centromere protein K causes anticancer effects in breast carcinoma via effects on the FAK/PI3K/AKT/mTOR pathway

    DOI: 10.1016/j.taap.2022.116232

    PMID: 36089000

  20. TOXICOLOGY AND APPLIED PHARMACOLOGY (2022) IF: 4.46
    Inhibition of PTPN21 has antitumor effects in glioma by restraining the EGFR/PI3K/AKT pathway

    DOI: 10.1016/j.taap.2022.116180

    PMID: 35907586

  21. INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 4.932
    Perilipin 5 protects against oxygen-glucose deprivation/reoxygenation-elicited neuronal damage by inhibiting oxidative stress and inflammatory injury via the Akt-GSK-3β-Nrf2 pathway

    DOI: 10.1016/j.intimp.2022.108718

    PMID: 35367744

  22. MOLECULAR NEUROBIOLOGY (2021) IF: 5.59
    Lipin1 Alleviates Autophagy Disorder in Sciatic Nerve and Improves Diabetic Peripheral Neuropathy

    DOI: 10.1007/s12035-021-02540-5

  23. INTERNATIONAL IMMUNOPHARMACOLOGY (2023) IF: 5.714
    C1q/tumor necrosis factor-related protein-6 exerts protective effects on myocardial ischemia–reperfusion injury through the modulation of the Akt-GSK-3β-Nrf2 signaling cascade

    DOI: 10.1016/j.intimp.2023.109678

  24. Frontiers in Oncology (2022) IF: 5.738
    PRKCI Mediates Radiosensitivity via the Hedgehog/GLI1 Pathway in Cervical Cancer

    DOI: 10.3389/fonc.2022.887139

    PMID: 35785194

  25. International Journal of Nanomedicine (2022) IF: 6.4
    Self-Assembled Nano-Peptide Hydrogels with Human Umbilical Cord Mesenchymal Stem Cell Spheroids Accelerate Diabetic Skin Wound Healing by Inhibiting Inflammation and Promoting Angiogenesis

    DOI: 10.2147/IJN.S363777

  26. Pharmaceutics (2022) IF: 6.525
    Preparation of DSPE-PEG-cRGD Modified Cationic Liposomes for Delivery of OC-2 shRNA and the Antitumor Effects on Breast Cancer

    DOI: 10.3390/pharmaceutics14102157

    PMID: 36297592

  27. INFLAMMATION RESEARCH (2022) IF: 6.986
    Loss of pleckstrin homology domain and leucine-rich repeat protein phosphatase 2 has protective effects on high glucose-injured retinal ganglion cells via the effect on the Akt–GSK–3β–Nrf2 pathway

    DOI: 10.1007/s00011-022-01680-1

  28. Oxidative Medicine and Cellular Longevity (2023) IF: 7.31
    Overexpression of PRDX2 in Adipose-Derived Mesenchymal Stem Cells Enhances the Therapeutic Effect in a Neurogenic Erectile Dysfunction Rat Model by Inhibiting Ferroptosis

    DOI: 10.1155/2023/4952857

    Sample: Adipose-Derived Mesenchymal Stem Cells (Adscs)
Show AllShorten

Reviews/Q&A

  • Show all
  • Reviews
  • Q&A
... Show All Show Less

People Also Bought

Apply for
*Product Name:
*Catalog Number:
*Name:
*Email:
*Message:
*Country:
*When will you use it?

*Captcha: