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Annexin V-APC/DAPI Apoptosis Kit

  • Cat.No.:E-CK-A258

To Purchase E-CK-A258

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Detection principle

Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Annexin V-labeled fluorescein bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscope. Nuclear dyes (PI, DAPI, 7-AAD) have a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. Due to late apoptosis or loss of membrane integrity in necrotic cells, nuclear dyes can enter cells to stain DNA, and when used in combination with Annexin V, cells in different apoptotic stages can be distinguished.

General Information

Application Annexin V
Detection method Fluorometric Method
Sample type Cell samples
Assay time 40min
Detection instrument Flow Cytometer
Dye Type APC
Ex/Em (nm) 650/660
Channel set APC
Other reagents required PBS(E-BC-R187), Deionized water
Storage 2~8°C, shading light
Shipping Ice bag
Expiration date 12 months

Citations

  1. Frontiers in Oncology (2022) IF: 6.244
    Mutant NPM1-Regulated FTO-Mediated m6A Demethylation Promotes Leukemic Cell Survival via PDGFRB/ERK Signaling Axis.

    DOI: 10.3389/fonc.2022.817584

  2. CANCER LETTERS (2021) IF: 8.679
    Circular RNA circSNX6 promotes sunitinib resistance in renal cell carcinoma through the miR-1184/GPCPD1/ lysophosphatidic acid axis

    DOI: 10.1016/j.canlet.2021.10.003

    PMID: 34626691

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