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Annexin V-APC/PI Apoptosis Kit

  • Cat.No.:E-CK-A217

To Purchase E-CK-A217

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Detection principle

Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Annexin V-labeled fluorescein bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscope. Nuclear dyes (PI, DAPI, 7-AAD) have a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. Due to late apoptosis or loss of membrane integrity in necrotic cells, nuclear dyes can enter cells to stain DNA, and when used in combination with Annexin V, cells in different apoptotic stages can be distinguished.

General Information

Application Annexin V
Detection method Fluorometric Method
Sample type Cell samples
Assay time 40min
Detection instrument Flow Cytometer
Dye Type APC
Ex/Em (nm) 650/660
Channel set APC
Other reagents required PBS(E-BC-R187), Deionized water
Storage 2~8°C, shading light
Shipping Ice bag
Expiration date 12 months

Citations

  1. Nature Communications (2022) IF: 14.919
    Targeting dual-specificity tyrosine phosphorylation-regulated kinase 2 with a highly selective inhibitor for the treatment of prostate cancer

    DOI: 10.1038/s41467-022-30581-4

    PMID: 35614066

  2. CHEMICO-BIOLOGICAL INTERACTIONS (2023) IF: 5.168
    Calycosin-7-glucoside promotes mitochondria-mediated apoptosis in hepatocellular carcinoma by targeting thioredoxin 1 to regulate oxidative stress

    DOI: 10.1016/j.cbi.2023.110411

  3. MOLECULAR NUTRITION & FOOD RESEARCH (2020) IF: 5.82
    Lactobacillus Protects Against S. Typhimurium–Induced Intestinal Inflammation by Determining the Fate of Epithelial Proliferation and Differentiation

    DOI: 10.1002/mnfr.201900655

    Sample: Cell Sample
  4. TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE (2022) IF: 1.848
    Trifluoperazine Synergistically Potentiates Bortezomib-Induced Anti-Cancer Effect in Multiple Myeloma via Inhibiting P38 MAPK/NUPR1

    DOI: 10.1620/tjem.2022.J044

    PMID: 35644544

  5. ENDOCRINE JOURNAL (2021) IF: 2.349
    Silencing long non-coding RNAs nicotinamide nucleotide transhydrogenase antisense RNA 1 inhibited papillary thyroid cancer cell proliferation, migration and invasion and promoted apoptosis via targeting miR-199a-5p

    DOI: 10.1507/endocrj.EJ20-0353

    PMID: 33612561

    Sample: Cell(Tpc-1 Or Ihh-4 Cell)
  6. International Journal of Medical Sciences (2020) IF: 2.523
    Down-regulation of Xist and Mir-7a-5p improves LPS-induced myocardial injury

    DOI: 10.7150/ijms.45408

    PMID: 33029099

    Sample: Cell Sample
  7. JOURNAL OF MOLECULAR HISTOLOGY (2019) IF: 2.611
    NFIC promotes the vitality and osteogenic differentiation of rat dental follicle cells

    DOI: 10.1007/s10735-019-09841-z

  8. TECHNOLOGY IN CANCER RESEARCH & TREATMENT (2022) IF: 2.876
    FOXF2 Regulates PRUNE2 Transcription in the Pathogenesis of Colorectal Cancer:

    DOI: 10.1177/15330338221118717

  9. International Journal of Laboratory Hematology (2021) IF: 2.877
    CD44 enhances adriamycin resistance in chronic myelogenous leukaemia cells K562

    DOI: 10.1111/ijlh.13455

    PMID: 33411349

  10. Photodiagnosis and Photodynamic Therapy (2022) IF: 3.577
    PARP inhibitors intervene DNA damage repair for the enhancement of tumor photodynamic therapy

    DOI: 10.1016/j.pdpdt.2022.103058

  11. CYTOKINE (2022) IF: 3.861
    SOX4-activated lncRNA MCM3AP-AS1 aggravates osteoarthritis progression by modulating miR-149-5p/Notch1 signaling

    DOI: 10.1016/j.cyto.2022.155805

    PMID: 35202986

  12. JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY (2022) IF: 3.981
    Curcumin-loaded zein/pectin nanoparticles: Caco-2 cellular uptake and the effects on cell cycle arrest and apoptosis of human hepatoma cells (HepG2)

    DOI: 10.1016/j.jddst.2022.103497

  13. BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545
    Knockdown of enhancer of rudimentary homolog inhibits proliferation and metastasis in ovarian cancer by regulating epithelial-mesenchymal transition

    DOI: 10.1016/j.biopha.2020.109974

    PMID: 32036222

    Sample: Cell Culture Supernatant
  14. Journal of Functional Biomaterials (2022) IF: 4.901
    On-Demand Release of Fucoidan from a Multilayered Nanofiber Patch for the Killing of Oral Squamous Cancer Cells and Promotion of Epithelial Regeneration

    DOI: 10.3390/jfb13040167

    PMID: 36278636

  15. Toxins (2022) IF: 5.075
    Analysis of the Roles of the ISLR2 Gene in Regulating the Toxicity of Zearalenone Exposure in Porcine Intestinal Epithelial Cells

    DOI: 10.3390/toxins14090639

    PMID: 36136577

  16. Aging-US (2019) IF: 5.515
    Circular RNA SMO sponges miR-338-3p to promote the growth of glioma by enhancing the expression of SMO

    DOI: 10.18632/aging.102576

  17. Frontiers in Oncology (2022) IF: 6.244
    Effective Delivery of siRNA-Loaded Nanoparticles for Overcoming Oxaliplatin Resistance in Colorectal Cancer.

    DOI: 10.3389/fonc.2022.827891

  18. Cancers (2022) IF: 6.575
    TFAP2C Knockdown Sensitizes Bladder Cancer Cells to Cisplatin Treatment via Regulation of EGFR and NF-κB

    DOI: 10.3390/cancers14194809

  19. PHARMACOLOGICAL RESEARCH (2022) IF: 7.658
    Cyclo-(Phe-Tyr) as a novel cyclic dipeptide compound alleviates ischemic/reperfusion brain injury via JUNB/JNK/NF-κB and SOX5/PI3K/AKT pathways

    DOI: 10.1016/j.phrs.2022.106230

  20. Advanced Science (2022) IF: 17.521
    Pyroptosis Remodeling Tumor Microenvironment to Enhance Pancreatic Cancer Immunotherapy Driven by Membrane Anchoring Photosensitizer

    DOI: 10.1002/advs.202202914

    PMID: 35981886

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