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One-step TUNEL In Situ Apoptosis Kit (Green, Elab Fluor^® 488)

Citations(19)

Cat.No.:E-CK-A321

Applications:Apoptosis/DNA Fragmentation

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Detection principle

When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with fluorescence microscope.

General Information

Application	DNA Fragmentation
Detection method	Fluorometric Method
Sample type	Paraffin section, frozen section, cell slide
Assay time	3 hours
Detection instrument	Fluorescence Microscope
Dye Type	Elab Fluor^® 488
Ex/Em (nm)	495/519
Channel set	FITC
Filter set	FITC
Other reagents required	4% Paraformaldehyde(E-IR-R113),0.2% Triton X-100(E-IR-R122),PBS(E-IR-R187),ddH2O,Mounting solution with anti-fluorescence quencher(E-IR-R119),Xylene,Ethanol
Storage	-20°C, shading light
Shipping	Ice bag
Expiration date	12 months

Related Products

E-CK-A320	One-step TUNEL In Situ Apoptosis Kit (Green, FITC)
E-CK-A211	Annexin V-FITC/PI Apoptosis Kit
E-CK-A301	Mitochondrial Membrane Potential Assay Kit (with JC-1)
E-CK-A362	Enhanced Cell Counting Kit 8 (WST-8/CCK8)

Operation Guide

Citations

CELL CYCLE (2022) IF: 5.173

Low-dose aspirin inhibits trophoblast cell apoptosis by activating the CREB/Bcl-2 pathway in pre-eclampsia

DOI: 10.1080/15384101.2022.2092814
PMID: 35792905

Biopreservation and Biobanking (2021) IF: 2.3

Effects of AavLEA1 Protein on Mouse Ovarian Tissue Cryopreservation by Vitrification

DOI: 10.1089/bio.2021.0048
PMID: 34788107

JOURNAL OF MOLECULAR HISTOLOGY (2021) IF: 2.611

The W9 peptide inhibits osteoclastogenesis and osteoclast activity by downregulating osteoclast autophagy and promoting osteoclast apoptosis

DOI: 10.1007/s10735-021-10030-0
PMID: 34664129

CRYOBIOLOGY (2022) IF: 2.728

Cryopreservation of human dental roots using vitrification for autologous human tooth tissue banking

DOI: 10.1016/j.cryobiol.2022.11.241

Experimental and Therapeutic Medicine (2022) IF: 2.751

Dual specificity phosphatase 22 suppresses mesangial cell hyperproliferation, fibrosis, inflammation and the MAPK signaling pathway in diabetic nephropathy

DOI: 10.3892/etm.2022.11680

International Journal of Genomics (2022) IF: 2.758

The Novel Action of miR-193b-3p/CDK1 Signaling in HCC Proliferation and Migration: A Study Based on Bioinformatic Analysis and Experimental Investigation

DOI: 10.1155/2022/8755263

HUMAN & EXPERIMENTAL TOXICOLOGY (2023) IF: 3.247

Sevoflurane Postconditioning Attenuates Cerebral Ischemia-Reperfusion Injury by Inhibiting SP1/ACSL4-Mediated Ferroptosis

DOI: 10.1177/09603271231160477

DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY (2022) IF: 3.605

Andrographis paniculata improves growth and non-specific immunity of shrimp Litopenaeus vannamei, and protects it from Vibrio alginolyticus by reducing oxidative stress and apoptosis

DOI: 10.1016/j.dci.2022.104542
PMID: 36122733

NEUROSCIENCE (2023) IF: 3.708

Peroxiredoxin-6 Released by Astrocytes Contributes to Neuroapoptosis During Ischemia

DOI: 10.1016/j.neuroscience.2023.01.003

BRAIN RESEARCH BULLETIN (2022) IF: 4.079

Sustained developmental endothelial locus-1 overexpression promotes spinal cord injury recovery in mice through the SIRT1/SERCA2 signaling pathway

DOI: 10.1016/j.brainresbull.2022.01.016
PMID: 35093467

Toxics (2022) IF: 4.472

Gestational Exposure to Cyfluthrin through Endoplasmic Reticulum (ER) Stress—Mediated PERK Signaling Pathway Impairs Placental Development

DOI: 10.3390/toxics10120733

Clinical and Translational Radiation Oncology (2022) IF: 4.739

Comparison of intratumor and local immune response between MV X-ray FLASH and conventional radiotherapies

DOI: 10.1016/j.ctro.2022.11.005

Reproductive Biology and Endocrinology (2022) IF: 4.982

hUCMSCs reduce theca interstitial cells apoptosis and restore ovarian function in premature ovarian insufficiency rats through regulating NR4A1-mediated mitochondrial mechanisms

DOI: 10.1186/s12958-022-00992-5
PMID: 35986315

PHYTOMEDICINE (2022) IF: 5.34

Dihydromyricetin protects against Doxorubicin-induced cardiotoxicity through activation of AMPK/mTOR pathway

DOI: 10.1016/j.phymed.2022.154027
PMID: 35278898

Journal of Translational Medicine (2022) IF: 5.531

The role of neutrophil elastase in aortic valve calcification

DOI: 10.1186/s12967-022-03363-1

Journal of Inflammation-London (2022) IF: 6.283

5-Methoxyflavone alleviates LPS-mediated lung injury by promoting Nrf2-mediated the suppression of NOX4/TLR4 axis in bronchial epithelial cells and M1 polarization in macrophages

DOI: 10.1186/s12950-022-00319-6

Stem Cell Research & Therapy (2022) IF: 8.079

UC-MSCs promote frozen-thawed ovaries angiogenesis via activation of the Wnt/β-catenin pathway in vitro ovarian culture system

DOI: 10.1186/s13287-022-02989-8
PMID: 35841074

Journal of Translational Medicine (2023) IF: 8.44

Angiotensin-(1–7) ameliorates sepsis-induced cardiomyopathy by alleviating inflammatory response and mitochondrial damage through the NF-κB and MAPK pathways

DOI: 10.1186/s12967-022-03842-5

JOURNAL OF NANOBIOTECHNOLOGY (2022) IF: 9.429

Self-assembly of DNA nanostructure containing cell-specific aptamer as a precise drug delivery system for cancer therapy in non-small cell lung cancer

DOI: 10.1186/s12951-022-01701-5

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Reviews/Q&A

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Reviews
Q&A

K*************iSubmitted [ Jun 05 2024 ]

Asked: Respected Sir I am using this kit for the first time, and I want to check whether it works on my samples. If possible, can I get a trial pack of this kit with cat number: E-CK-A321. Thanks

adminSubmitted [ Jun 05 2024 ]

Answered: We will reply you by E-mail later.Please check your mailbox.

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