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One-step TUNEL In Situ Apoptosis Kit (Red, Elab Fluor^® 555)

Citations(7)

Cat.No.:E-CK-A325

Applications:Apoptosis/DNA Fragmentation

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Detection principle

When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with fluorescence microscope.

General Information

Application	DNA Fragmentation
Detection method	Fluorometric Method
Sample type	Paraffin section, frozen section, cell slide
Assay time	3 hours
Detection instrument	Fluorescence Microscope
Dye Type	Elab Fluor^® 555
Ex/Em (nm)	555/565
Filter set	TRITC
Other reagents required	4% Paraformaldehyde(E-IR-R113),0.2% Triton X-100(E-IR-R122),PBS(E-IR-R187),ddH2O,Mounting solution with anti-fluorescence quencher(E-IR-R119),Xylene,Ethanol
Storage	-20°C, shading light
Shipping	Ice bag
Expiration date	12 months

Related Products

E-CK-A321	One-step TUNEL In Situ Apoptosis Kit (Green, Elab Fluor^® 488)
E-CK-A211	Annexin V-FITC/PI Apoptosis Kit
E-CK-A301	Mitochondrial Membrane Potential Assay Kit (with JC-1)
E-CK-A362	Enhanced Cell Counting Kit 8 (WST-8/CCK8)

Operation Guide

Citations

Biopreservation and Biobanking (2021) IF: 2.3

Effects of AavLEA1 Protein on Mouse Ovarian Tissue Cryopreservation by Vitrification

DOI: 10.1089/bio.2021.0048
PMID: 34788107

Molecular Genetics & Genomic Medicine (2022) IF: 2.473

The compound heterozygous mutations of c.607G>a and c.657delC in the FAH gene are associated with renal damage with hereditary tyrosinemia type 1 (HT1)

DOI: 10.1002/mgg3.2090

Journal of Stroke & Cerebrovascular Diseases (2023) IF: 2.677

Inhibition of PI3K/Akt/mTOR signaling by NDRG2 contributes to neuronal apoptosis and autophagy in ischemic stroke

DOI: 10.1016/j.jstrokecerebrovasdis.2023.106984

RENAL FAILURE (2022) IF: 3.222

Rosiglitazone attenuates hypoxia-induced renal cell apoptosis by inhibiting NF-κB signaling pathway in a PPARγ-dependent manner

DOI: 10.1080/0886022X.2022.2148539

Biomedicines (2022) IF: 4.757

Tauroursodeoxycholic Acid Reduces Neuroinflammation but Does Not Support Long Term Functional Recovery of Rats with Spinal Cord Injury

DOI: 10.3390/biomedicines10071501
PMID: 35884805

DRUG DELIVERY (2022) IF: 6.419

Sevelamer arsenite nanoparticle as a Pi-responsive drug carrier and embolic agent for chemoembolization

DOI: 10.1080/10717544.2022.2072541

Nature Communications (2022) IF: 17.694

Long-term monitoring of intravital biological processes using fluorescent protein-assisted NIR-II imaging

DOI: 10.1038/s41467-022-34274-w
PMID: 36333308

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Reviews/Q&A

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Reviews
Q&A

A*********ySubmitted [ Jul 21 2023 ]

Asked: can I use this kit for bone tissue cartilage tissue? If not please sugest me a better one.

adminSubmitted [ Jul 21 2023 ]

Answered: Hello Anirban,thanks for your support.We will reply you via E-mail.

S*******eSubmitted [ Jul 20 2022 ]

Asked: Can someone explain how this kit can be used to visualize apoptosis in Drosophila melanogaster tissues?

adminSubmitted [ Jul 20 2022 ]

Answered: this kit can detect animal paraffin section, frozen section, cell slide. But insect sample is not verified in our lab. If you need to use insect samples for testing with this kit, I suggest to conduct pre-experiment to exploreteh sample preparation after consulting articles.

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