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One-step TUNEL In Situ Apoptosis Kit (Red, Elab Fluor® 594)

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  • Cat.No.:E-CK-A322

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Detection principle

When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with fluorescence microscope.

General Information

Application DNA Fragmentation
Detection method Fluorometric Method
Sample type Paraffin section, frozen section, cell slide
Assay time 3 hours
Detection instrument Fluorescence Microscope
Dye Type Elab Fluor® 594
Ex/Em (nm) 590/617
Filter set TRITC
Other reagents required 4% Paraformaldehyde(E-IR-R113),0.2% Triton X-100(E-IR-R122),PBS(E-IR-R187),ddH2O,Mounting solution with anti-fluorescence quencher(E-IR-R119),Xylene,Ethanol
Storage -20°C, shading light
Shipping Ice bag
Expiration date 12 months

Operation Guide

Citations

  1. JOURNAL OF ETHNOPHARMACOLOGY (2023) IF: 5.195
    The therapeutic mechanism of Yuye decoction on type 2 diabetes mellitus based on network pharmacology and experimental verification

    DOI: 10.1016/j.jep.2023.116222

  2. Frontiers in Pharmacology (2022) IF: 5.988
    Monomethyl lithospermate alleviates ischemic stroke injury in middle cerebral artery occlusion mice in vivo and protects oxygen glucose deprivation/reoxygenation induced SHSY-5Y cells in vitro via activation of PI3K/Akt signaling.

    DOI: 10.3389/fphar.2022.1024439

    Sample: Tissue Slice
  3. Biopreservation and Biobanking (2021) IF: 2.3
    Effects of AavLEA1 Protein on Mouse Ovarian Tissue Cryopreservation by Vitrification

    DOI: 10.1089/bio.2021.0048

    PMID: 34788107

  4. Journal of Stroke & Cerebrovascular Diseases (2023) IF: 2.677
    Inhibition of PI3K/Akt/mTOR signaling by NDRG2 contributes to neuronal apoptosis and autophagy in ischemic stroke

    DOI: 10.1016/j.jstrokecerebrovasdis.2023.106984

  5. RENAL FAILURE (2022) IF: 3.222
    Rosiglitazone attenuates hypoxia-induced renal cell apoptosis by inhibiting NF-κB signaling pathway in a PPARγ-dependent manner

    DOI: 10.1080/0886022X.2022.2148539

  6. Frontiers in Pediatrics (2022) IF: 3.418
    Hyperoxia Induced Bronchopulmonary Dysplasia-Like Inflammation via miR34a-TNIP2-IL-1β Pathway

    DOI: 10.3389/fped.2022.805860

    Sample: Sputum
  7. TOXICOLOGY LETTERS (2023) IF: 4.271
    The mechanism of rh-endostatin-induced cardiotoxicity and its protection by dihydromyricetin[in vivo/in vitro, C57BL/6 mice, AC16 and hiPSC-CMs]

    DOI: 10.1016/j.toxlet.2023.01.012

  8. DRUG DELIVERY (2022) IF: 6.419
    Sevelamer arsenite nanoparticle as a Pi-responsive drug carrier and embolic agent for chemoembolization

    DOI: 10.1080/10717544.2022.2072541

  9. Stem Cell Research & Therapy (2022) IF: 8.079
    miR-126-3p containing exosomes derived from human umbilical cord mesenchymal stem cells promote angiogenesis and attenuate ovarian granulosa cell apoptosis in a preclinical rat model of premature ovarian failure

    DOI: 10.1186/s13287-022-03056-y

    PMID: 35883161

  10. JOURNAL OF NANOBIOTECHNOLOGY (2022) IF: 9.429
    Platelet membrane-camouflaged nanoparticles carry microRNA inhibitor against myocardial ischaemia‒reperfusion injury

    DOI: 10.1186/s12951-022-01639-8

    PMID: 36195952

  11. Nature Communications (2022) IF: 17.694
    Long-term monitoring of intravital biological processes using fluorescent protein-assisted NIR-II imaging

    DOI: 10.1038/s41467-022-34274-w

    PMID: 36333308

  12. ACS Nano (2023) IF: 18.027
    Supramolecular Hydrogel Microspheres of Platelet-Derived Growth Factor Mimetic Peptide Promote Recovery from Spinal Cord Injury

    DOI: 10.1021/acsnano.2c12017

    PMID: 36787636

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