When cells undergo apoptosis, endonuclease enzymes are activated to cleave the genomic DNA between nucleosomes. When genomic DNA is cleaved, the exposed 3'-OH can be added with a biotinylated dUTP (Biotin-dUTP) due to the catalysis of Terminal Deoxynucleotidyl Transferase (TdT), followed by the binding of horseradish Peroxidase-labeled Streptavidin (Streptavidin-HRP). Thereafter, through the HRP catalysis, the apoptotic cells are marked by DAB, which can be detected by ordinary microscopy. This is the principle of TUNEL (TdT-mediated dUTP Nick-End Labeling) for detecting apoptosis.

General Information

Application	DNA Fragmentation
Detection method	HRP-DAB
Sample type	Paraffin section, frozen section, cell slide
Assay time	3 hours
Detection instrument	Light microscope
Other reagents required	4% Paraformaldehyde(E-IR-R113),0.2% Triton X-100(E-IR-R122),3% H2O2(E-IR-R115),Xylene，Ethanol，DNase I，PBS(E-IR-R187),ddH2O,Hematoxylin(E-IR-R120)，Neutral gum(E-IR-R118)
Storage	-20°C, shading light. Refer to the Manual.
Shipping	Ice bag
Expiration date	12 months

Related Products

E-CK-A320	One-step TUNEL In Situ Apoptosis Kit (Green, FITC)
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E-CK-A301	Mitochondrial Membrane Potential Assay Kit (with JC-1)
E-CK-A362	Enhanced Cell Counting Kit 8 (WST-8/CCK8)

Citations

Journal of Translational Medicine (2022) IF: 8.44

Effects of hydrogen-rich saline in neuroinflammation and mitochondrial dysfunction in rat model of sepsis-associated encephalopathy

DOI: 10.1186/s12967-022-03746-4

Cells (2022) IF: 7.666

Regulation Networks of Non-Coding RNA-Associated ceRNAs in Cisplatin-Induced Acute Kidney Injury

DOI: 10.3390/cells11192971
PMID: 36230932

Frontiers in Bioengineering and Biotechnology (2022) IF: 6.064

Scutellarin Protects Against Mitochondrial Reactive Oxygen Species-Dependent NLRP3 Inflammasome Activation to Attenuate Intervertebral Disc Degeneration.

DOI: 10.3389/fbioe.2022.883118

Journal of Inflammation Research (2021) IF: 6.922

Endothelin-1/Endothelin Receptor Type A-Angiopoietins/Tie-2 Pathway in Regulating the Cross Talk Between Glomerular Endothelial Cells and Podocytes in Trichloroethylene-Induced Renal Immune Injury

DOI: 10.2147/JIR.S301104
	Sample: Serum,Plasma,Urine

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2021) IF: 5.31

Oxidized high-density lipoprotein enhances endocrine disorders and ovarian damage in rats

DOI: 10.1111/jcmm.16197
PMID: 34346538

INTERNATIONAL IMMUNOPHARMACOLOGY (2021) IF: 4.932

Influence of hyperocclusion on the remodeling of gingival tissues

DOI: 10.1016/j.intimp.2021.107885

CELL BIOLOGY INTERNATIONAL (2022) IF: 3.612

Rifaximin protects SH-SY5Y neuronal cells from iron overload-induced cytotoxicity via inhibiting STAT3/NF-κB signaling

DOI: 10.1002/cbin.11776
PMID: 35143099

JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY (2021) IF: 3.849

The protective effect of zinc on morphine-induced testicular toxicity via p53 and Akt pathways: An in vitro and in vivo approach

DOI: 10.1016/j.jtemb.2021.126776
PMID: 33984544	Sample: Cell culture supernatant

Medicina-Lithuania (2021) IF: 2.43

May Argyrophilic Nucleolar Organizer Regions Be Used as a Biomarker for the Detection of the Degree of Ischemic Damage Instead of Tunel in Testicular Torsion?

DOI: 10.3390/medicina57111177

JOURNAL OF MOLECULAR HISTOLOGY (2021) IF: 2.611

Prednisolone induces osteocytes apoptosis by promoting Notum expression and inhibiting PI3K/AKT/GSK3β/β-catenin pathway

DOI: 10.1007/s10735-021-10006-0
PMID: 34297260

BIOLOGICAL TRACE ELEMENT RESEARCH (2020) IF: 2.639

In Vitro and In Vivo Pretreatment with Selenium Mitigates Tetrahydrocannabinol-Induced Testicular Cell Apoptosis: the Role of AKT and p53 Pathways

DOI: 10.1007/s12011-020-02322-5
PMID: 32815089	Sample: Cell sample

JOURNAL OF BIOENERGETICS AND BIOMEMBRANES (2021) IF: 2.945

Silencing circRNA LRP6 down‐regulates PRMT1 to improve the streptozocin‐induced pancreatic β-cell injury and insulin secretion by sponging miR-9-5p

DOI: 10.1007/s10863-021-09895-3
	Sample: Cell sample

PHARMAZIE (2021) IF: 1.267

The effects of the renin inhibitor aliskiren on neuronal toxic damage

DOI: 10.1691/ph.2021.1729

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