Click here to view cell apoptosis assay products

Western Blot Detection Kit

    • Western Blot Detection Kit-Elabscience
    • Western Blot Detection Kit-Elabscience
    • Western Blot Detection Kit-Elabscience
    <
    >
    • Western Blot Detection Kit-Elabscience
    • Western Blot Detection Kit-Elabscience
    • Western Blot Detection Kit-Elabscience
    • Western Blot Detection Kit-Elabscience
    • Western Blot Detection Kit-Elabscience
    • Western Blot Detection Kit-Elabscience

      Catalog number:E-IR-R304A

      Size:
      • 50 Assays
      Qty:
      - +
      Price: $210

      Lead Time:Order now, ship in 3 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Western Blot Kit (E-IR-R304)

      Western Blot (WB) is the most common and classical experimental method for detecting protein expression in the biological science. However, due to differences in reagent sources, methods of operation and experience used by different Laboratory Technician, a large number of WB experimental results do not meet the requirement of paper publishing. Elabscience has been focusing on the field of immunology for many years and has accumulated a lot of experience in WB operation. In order to help researchers solve the above problems, the Western Blot kit is now available. The reagents in this kit are provided for classical WB experiments, including all reagents needed from protein extraction to result detection, which have advantages of simple operation, high detection sensitivity, low background and strong system stability. And Elabscience provides detailed operation description and professional technical support. Additionally, it offers you convenience and guarantee to work out ideal Western Blot experiment results.

       

      As the PVDF membranes have two pore sizes for different molecular weights of target proteins respectively. In order to meet different experimental requirements, we have designed two specifications of Western Blot kits.

      E-IR-R304A (PVDF membrane, 0.45 μm) is recommended for the proteins detection with molecular weights more than 20 kDa.

      E-IR-R304B (PVDF membrane, 0.22 μm) is recommended for the proteins detection with molecular weights less than 20 kDa.

       

      • E-IR-R304A

      E-IR-R304A (PVDF membrane, 0.45 μm) is recommended for the proteins detection with molecular weights more than 20 kDa.

      Provided product

      Product Catalog No Specification Storage Shelf life
      RIPA Lysis Buffer (with PMSF and Na3VO4) E-BC-R327 5 mL/50 μL+50 μL -20℃ 12 months
      Total Protein Colorimetric Assay Kit (BCA method) E-BC-K318 48 T RT 12 months
      Excellent Chemiluminescent Substrate Detection Kit E-IR-R307 15 mL/15 mL 4℃ 12 months
      Pre-stained Protein Marker (10-180 kDa) E-BC-R273 25 µL -20℃ 12 months
      Goat Anti-Rabbit IgG (H+L) (peroxidase/HRP conjugated) E-AB-1003 20 µL -20℃ 12 months
      Skim Milk Powder E-BC-R337 15 g RT 12 months
      PBS Buffer, pH7.4 (10 ×) E-BC-R187 100 mL RT 12 months
      5 × SDS Loading Buffer E-BC-R288 1 mL × 2 -20℃ 12months
      Electrophoresis Buffer (10 ×) E-BC-R331 125 mL × 2 RT 12 months
      Transmembrane Buffer (10 ×) E-BC-R333 125 mL × 2 4℃ 12 months
      TBST Buffer, pH7.4 (10 ×) E-BC-R335 125 mL × 2 4℃ 12 months
      PVDF Membrane (0.45 μm, 8.5 × 6 cm) E-BC-R266 5 pieces RT 12 months

       

      Alternative Reagent offer

      Product Catalog No Specification Storage Shelf life
      Goat Anti-Mouse IgG(H+L) (peroxidase/HRP conjugated) E-AB-1001 20 µL -20℃ 12 months

       

      Self-Prepared Reagent:Methanol

       

      Instructions for user

      This kit is for five gels (about 45 samples) with the Western Blot experiment.

      Please store the reagents according to the storage conditions provided in the manual.

      Citations

      1. Overexpression of Long Non-Coding RNA MIR22HG Represses Proliferation and Enhances Apoptosis via miR-629-5p/TET3 Axis in Osteosarcoma Cells
        IF: 2.351
        Journal: JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY(2021)
        DOI: 10.4014/jmb.2106.06028
        PMID: 34373436
        Products: E-IR-R304A 
      2. Essential Role Of High Glucose-Induced Overexpression Of PKCβ And PKCδ In GLP-1 Resistance In Rodent Cardiomyocytes
        IF: 3.319
        Journal: Diabetes Metabolic Syndrome and Obesity-Targets and Therapy(2019)
        DOI: 10.2147/DMSO.S215789
        Products: E-IR-R304A 
      3. Novel benzo chromene derivatives: design, synthesis, molecular docking, cell cycle arrest, and apoptosis induction in human acute myeloid leukemia HL-60 cells
        IF: 5.756
        Journal: JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY(2022)
        DOI: 10.1080/14756366.2022.2151592
        Products: E-IR-R304A 
      4. Activatable Self-Dissociation of Watson–Crick Structures with Fluorescent Nucleotides for Sensing Multiple Human Glycosylases at Single-Cell Level
        IF: 8.008
        Journal: ANALYTICAL CHEMISTRY(2022)
        DOI: 10.1021/acs.analchem.2c04624
        Products: E-IR-R304A 
      5. Controllable Autocatalytic Cleavage-Mediated Fluorescence Recovery for Homogeneous Sensing of Alkyladenine DNA Glycosylase from Human Cancer Cells
        IF: 8.063
        Journal: Theranostics(2019)
        DOI: 10.7150/thno.35393
        Products: E-IR-R304A 
      6. Construction of a self-directed replication system for label-free and real-time sensing of repair glycosylases with zero background
        IF: 9.556
        Journal: Chemical Science(2019)
        DOI: 10.1039/C9SC04738G
        Products: E-IR-R304A 
      7. A controlled T7 transcription-driven symmetric amplification cascade machinery for single-molecule detection of multiple repair glycosylases
        IF: 9.825
        Journal: Chemical Science(2021)
        DOI: 10.1039/D1SC00189B
        PMID: 34168791
        Products: E-IR-R304A 
        Sample type: Cell Lysate
        Reactivity: human
      8. A novel multi-functionalized multicellular nanodelivery system for non-small cell lung cancer photochemotherapy
        IF: 10.435
        Journal: JOURNAL OF NANOBIOTECHNOLOGY(2021)
        DOI: 10.1186/s12951-021-00977-3
        PMID: 34391438
        Products: E-IR-R304A 
      Show AllShorten
      • Show all (1)
      • Reviews (0)
      • Q&A (1)
      Q

      g*********kSubmitted [ Jun 11 2024 ]

      Asked: Can we use 96% ethanol instead of methanol in this kit?

      Reply

      A

      adminSubmitted [ Jun 11 2024 ]

      Answered: Dear Gul Kozalak, 96% ethanol canot not replace methanol as it can not acitivate PVDF membrane, please follow the instruction. Regards, Cathy

      ... Show All Show Less
      MSDS for ELISA ISO 9001

      Browsing History


        People Also Bought

        Apply for
        *Product Name:
        *Catalog Number:
        *Name:
        *Email:
        *Message:
        *Country:
        *When will you use it?

        *Captcha: