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High Accuracy and Absorbability Western Blot Detection Kit

    • High Accuracy and Absorbability Western Blot Detection Kit-Elabscience
    • High Accuracy and Absorbability Western Blot Detection Kit-Elabscience
    • High Accuracy and Absorbability Western Blot Detection Kit-Elabscience
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    • High Accuracy and Absorbability Western Blot Detection Kit-Elabscience
    • High Accuracy and Absorbability Western Blot Detection Kit-Elabscience
    • High Accuracy and Absorbability Western Blot Detection Kit-Elabscience
    • High Accuracy and Absorbability Western Blot Detection Kit-Elabscience
    • High Accuracy and Absorbability Western Blot Detection Kit-Elabscience
    • High Accuracy and Absorbability Western Blot Detection Kit-Elabscience

      Catalog number:E-IR-R304B

      Size:
      • 50 Assays
      Qty:
      - +
      Price: $230

      Lead Time:Order now, ship in 3 daysWelcome to order from local distributors.

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      Western Blot Kit (E-IR-R304)

      Western Blot (WB) is the most common and classical experimental method for detecting protein expression in the biological science. However, due to differences in reagent sources, methods of operation and experience used by different Laboratory Technician, a large number of WB experimental results do not meet the requirement of paper publishing. Elabscience has been focusing on the field of immunology for many years and has accumulated a lot of experience in WB operation. In order to help researchers solve the above problems, the Western Blot kit is now available. The reagents in this kit are provided for classical WB experiments, including all reagents needed from protein extraction to result detection, which have advantages of simple operation, high detection sensitivity, low background and strong system stability. And Elabscience provides detailed operation description and professional technical support. Additionally, it offers you convenience and guarantee to work out ideal Western Blot experiment results.

       

      As the PVDF membranes have two pore sizes for different molecular weights of target proteins respectively. In order to meet different experimental requirements, we have designed two specifications of Western Blot kits.

      E-IR-R304A (PVDF membrane, 0.45 μm) is recommended for the proteins detection with molecular weights more than 20 kDa.

      E-IR-R304B (PVDF membrane, 0.22 μm) is recommended for the proteins detection with molecular weights less than 20 kDa.

       

      • E-IR-R304B

      E-IR-R304B (PVDF membrane, 0.22 μm) is recommended for the proteins detection with molecular weights less than 20 kDa.

      Provided product

      Product Catalog No Specification Storage Shelf life
      RIPA Lysis Buffer (with PMSF and Na3VO4) E-BC-R327 5 mL/50 μL+50 μL -20℃ 12 months
      Total Protein Colorimetric Assay Kit (BCA method) E-BC-K318 48 T  RT 12 months
      Excellent Chemiluminescent Substrate Detection Kit E-IR-R307 15 mL/15 mL 4℃ 12 months
      Pre-stained Protein Marker (10-180 kDa) E-BC-R273 25 µL -20℃ 12 months
      Goat Anti-Rabbit IgG (H+L) (peroxidase/HRP conjugated) E-AB-1003 20 µL -20℃ 12 months
      Skim Milk Powder E-BC-R337 15 g RT 12 months
      PBS Buffer, pH7.4 (10×) E-BC-R187 100 mL RT 12 months
      5× SDS Loading Buffer E-BC-R288 1 mL × 2 -20℃ 12 months
      Electrophoresis Buffer (10×) E-BC-R331 125 mL × 2 RT 12 months
      Transmembrane Buffer (10×) E-BC-R333 125 mL × 2 4℃ 12 months
      TBST Buffer, pH7.4 (10×) E-BC-R335 125 mL × 2 4℃ 12 months
      PVDF Membrane (0.22 μm, 8.5 × 6 cm) E-BC-R329 5 pieces RT 12 months

       

      Alternative Reagent offer

      Product Catalog No Specification Storage Shelf life
      Goat Anti-Mouse IgG(H+L) (peroxidase/HRP conjugated) E-AB-1001 20 µL -20℃ 12 months

       

      Self-Prepared Reagent:Methanol

       

      Instructions for user

      This kit is for five gels (about 45 samples) with the Western Blot experiment.

      Please store the reagents according to the storage conditions provided in the manual.

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